Synthesis of strong polycations with improved biological properties

Wytrwał-Sarna, Magdalena; Koczurkiewicz, Paulina; Wójcik, Kinga; Michalik, Marta; Kozik, Bartłomiej; Zylewski, Marek; Nowakowska, Maria; Kępczyński, Mariusz

doi:10.1002/jbm.a.34744

Cited by 28 publications

(42 citation statements)

References 33 publications

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“…s4). These results are in line with previously reported findings, that the toxicity of PAH can be reduced by transforming its primary amino groups into the quaternary ammonium ones [20]. Moreover, the attachment of hydrophobic hexyl groups (PAHex) resulted in a further increase of the cell viability.…”

Section: Cytotoxicity Of the Polyelectrolytes In Solutionsupporting

confidence: 93%

“…It is the expected result, since this polymer is well known for its high cytotoxicity against various cell lines, e.g. human fibroblasts [20], human cervix epithelial carcinoma HeLa, human kidney cells 293T, and human hepatoblastoma HepG2 [22].…”

Section: Cytotoxicity Of the Polyelectrolytes In Solutionmentioning

confidence: 79%

“…1. Strong polycations, poly (allyltrimethylammonium chloride) (PATA) and poly(allyltrimethylammonium chloride-co-allyl-N,N-dimethyl-N-hexylammonium chloride) (PAHex) were prepared according to the procedures reported previously [20,21]. Both polymers have quaternary ammonium groups.…”

Section: Poly(allylamine Hydrochloride) Derivativesmentioning

confidence: 99%

“…The cell trajectories were depicted in circular diagrams with the starting point of each trajectory situated at the diagram center. The parameters characterizing cell locomotion were calculated as described previously [20]. Statistical significance was determined using a non-parametric Mann-Whitney U-test with p < 0.05 considered to indicate significant differences.…”

Section: Cell Migrationmentioning

confidence: 99%

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Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films

Wytrwał-Sarna

Koczurkiewicz

Źrubek

et al. 2016

Journal of Colloid and Interface Science

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Section: Cytotoxicity Of the Polyelectrolytes In Solutionsupporting

confidence: 93%

Section: Cytotoxicity Of the Polyelectrolytes In Solutionmentioning

confidence: 79%

Section: Poly(allylamine Hydrochloride) Derivativesmentioning

confidence: 99%

Section: Cell Migrationmentioning

confidence: 99%

See 2 more Smart Citations

Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films

Wytrwał-Sarna

Koczurkiewicz

Źrubek

et al. 2016

Journal of Colloid and Interface Science

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“…42 PAH has high toxicity toward various mammalian cells. 43 Although chitosan has good biocompatibility and antibacterial activity, 44 its physical properties are highly pH dependent. 45 To overcome the poor solubility, water soluble oligochitosan has been made by hydrolysis of chitosan.…”

Section: Introductionmentioning

confidence: 99%

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Huang¹,

Kumari²,

Herold³

et al. 2020

IJN

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Purpose: Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Methods: Se NPs were coated with a positively charged proteinrecombinant spider silk protein eADF4(κ16)to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4(κ16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Results: Compared to the negatively charged polyvinyl alcohol coated Se NPs, the positively charged eADF4(κ16)-coated Se NPs demonstrated a much higher bactericidal efficacy against the Gram-negative bacteria E. coli, with a minimum bactericidal concentration (MBC) approximately 50 times lower than that of negatively charged Se NPs. Cytotoxicity testing showed that the eADF4(κ16)-coated Se NPs are safe to both Balb/3T3 mouse embryo fibroblasts and HaCaT human skin keratinocytes up to 31 µg/mL, which is much higher than the MBC of these particles against E. coli (8 ± 1 µg/mL). In addition, antibacterial coatings were created by immobilising the eADF4(κ16)-coated Se NPs on positively charged spider silk films and these were shown to retain good bactericidal efficacy and overcome the issue of low particle stability in culture broth. It was found that these Se NPs needed to be released from the film surface in order to exert their antibacterial effects and this release can be regulated by the surface charge of the film, such as the change of the spider silk protein used. Conclusion: Overall, eADF4(κ16)-coated Se NPs are promising new antibacterial agents against life-threatening bacteria.

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Polyelectrolyte Substrate Coating for Controlling Biofilm Growth at Solid–Air Interface

Ryzhkov

Никитина

Fratzl

et al. 2021

Adv Materials Inter

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consequences on human health [1,2] and industrial processes [3,4] as well as antibiotics resistance. [5] Various approaches to inhibition of biofilm formation and their eradication were developed. [6] Biofilm formation at an interface is determined by several pheno mena, e.g., the initial adhesion of single bacteria to the surface and the further biofilm growth and spreading due to cell proliferation and biopolymer production. [7] Therefore, surface energy and electrostatic interactions with the substrate are two key determinants of biofilm formation. On this basis, a common strategy to fight bacterial colonization is the functionalization of surfaces that are prone to biofilm fouling. [6,8] For instance, surface hydrophilization [9] and low surface energy strategies [10] were considered. Surface topology was also modified to influence bacterial colonization, as recently reviewed in detail. [11] Polyelectrolyte coatings allow changing the surface energy, charge, and mechanical characteristics of various substrates easily. [12,13] Therefore, polyelectrolyte assemblies are often considered for possible application as antibacterial coatings. However, understanding the fundamental principles of bacteria-surface interactions remains of high importance. [14][15][16] Due to the negative charges found on their external membrane, bacteria tend to tightly attach to positively charged surfaces. [17] It was demonstrated that biofilm spreading rate was decreasing with increasing strength of adhesion. [17] One possible explanation is that bacteria elongation preceding cell division is obstructed by strong electrostatic binding to the surface. [18] Besides this, it is also known that positively charged molecules (and poly cations to a greater extent) exhibit antibacterial properties due to their ability to disrupt membranes of bacterial cells. [19] In contrast, negatively charged surfaces provide less stable bacterial cell contact with the surface, [18] so that the initial adhesion step is difficult but further biofilm spreading meets less obstacle.In addition to help preventing healthcare and industryrelated issues, investigating biofilm formation is beneficial to understand the development of biological tissues, [20] cell adaptability, [21] and communication [22] since some morphogenesis principles are common with higher organisms' tissues. Furthermore, colonies and biofilms of non-pathogenic microorganisms are considered to be promising to design hybrid living materials [23][24][25][26][27] challenging to get synthetically. Revealing

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Synthesis of strong polycations with improved biological properties

Cited by 28 publications

References 33 publications

Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films

Growth and motility of human skin fibroblasts on multilayer strong polyelectrolyte films

<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>

Polyelectrolyte Substrate Coating for Controlling Biofilm Growth at Solid–Air Interface

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