Synthesis, self-aggregation and cryopreservation effects of perylene bisimide–glycopeptide conjugates

Abstract

Three perylene bisimide-glycopeptide conjugates (PBI-AFF-Man, PBI-AFF-Glu and PBI-AFF-Gal) were synthesized, which showed moderately active in the control of ice crystal growth. Furthermore, the cellular cryopreservation effects of PBI-AFF-Man, PBI-AFF-Glu and...

Calixarene-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

“…However, the cell viability decreased at the dose of 1.0 mg mL À1 ; this phenomenon was also found in our previous work. 31 At the doses of 0.25 and 0.5 mg mL À1 , the enhanced cell viability of the calixarene-based CPAs showed a consistent trend with the IRI effect. SBAC4A showed the best cell cryopreservation effect in the tested cells, which was 16.16 AE 1.78%, 12.60 AE 1.15% and 14.90 AE 1.66% against MCF-7, hucMSCs and A549 cells at the dose of 0.5 mg mL À1 , respectively.…”

“…6c and d, SC4A-DMSO solution and SBAC4A -DMSO solution showed a different trend compared with the DMSO solution under a different heating temperature rate, which was consistent with the reported result that the heating and freezing rates exhibited an important influence on the ice crystals and the cryopreservation of the cells. 31 A low DMSO showed a synergistic effect with additional calixarene-based CPAs to reduce osmotic stress during freezing by replacing intracellular water. 29…”

Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

“…Then, the adherent cells and dead cells were counted, and the proportion of surviving cells compared to the total number of cells was calculated. 30 As shown in Fig. 5, the cell viabilities after treatment with macrocycle-based cryoprotectants were higher than those of the only DMSO group at doses of 0.25 mg mL −1 and 0.5 mg mL −1 , showing dose-dependent enhancement.…”

“…DMSO is a typical and gold-standard cryoprotectant, 29 and a 10% or 5% DMSO solution is usually used as a control group. 30 It is known that minimization or obviation of the use of DMSO is important to developing novel CPAs. 5 As a result, 2.5% DMSO solution was used as a control group.…”

Quality Assessment of Medicinal Plants via Chemometric Exploration of Quantitative NMR Data: A Review

“…These difficulties are worsened in systems with individual components that are uncertain or difficult to isolate and analyze. These systems are mixtures of Compounds 2022, 2 164 compounds in many states of order and disorder, multiphase materials, interfaces, dopants, biological systems that respond differently when isolated, and metabolomes [2]. NMR spectroscopy is a good tool for examining these complicated mixtures since it can quantitatively evaluate the specification of a bulk sample while also revealing information regarding the atomic environment, the ordering within the sample, and the electronic structure [3].…”