Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Karami, Kazem; Shirani-Sarmazeh, Zahra; Hosseini-Kharat, Mahboubeh; Lipkowski, Janusz; Saeidifar, Maryam

doi:10.1016/j.jphotobiol.2015.01.012

Cited by 23 publications

(10 citation statements)

References 68 publications

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“…To further investigate the interaction mode of Schiff base ligand (L) and complexes (1)–(4) with DNA, a competitive binding experiment using EtBr as a probe was carried out. EtBr is a common fluorescent probe for DNA due to its strong intercalation between the adjacent DNA base pairs . In general, the addition of a second DNA‐binding molecule would quench EtBr emission by replacing the DNA‐bound EtBr.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand

Dhayabaran¹,

Prakash²

2017

Luminescence

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A novel Schiff base, (S,Z)-4-(methylthio)-2-((3-oxo-2,3-dihydro-1H-inden-1-ylidene)amino)butanoic acid (L) and four M(II) complexes (where M = Co, Cu, Ni and Zn) were synthesized and characterized. The DNA-binding characteristics of the complexes were investigated using various spectroscopic methods and viscosity measurements. Analysis of the results suggests that all the complexes bind to calf thymus DNA via intercalation. Among the four, Cu(II) complex was found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm. These complexes also exhibit good antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl radical. In vitro antibacterial and antifungal assay indicates that these complexes are good antimicrobial agents.

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Section: Resultsmentioning

confidence: 99%

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand

Dhayabaran¹,

Prakash²

2017

Luminescence

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“…Cytotoxicity as IC 50 for each cell line is the concentration of compound that is reduced by 50% of the optical density of treated cells with respect to untreated cells using the MTT assay. The interaction of these complexes with CT-DNA and BSA was investigated using UV-Vis and fluorescence spectroscopic methods.…”

Section: Discussionmentioning

confidence: 99%

“…The response parameter was calculated in terms of IC 50 value, which corresponds to the concentration required for 50% inhibition of cell viability, and the results are summarized in Table 6. The in vitro anticancer screening results revealed that the synthesized compounds have shown significant to moderate activity against all three We also screened for their cytotoxicity against HEK-293 (human embryonic kidney 293) using MTT-micro-cultured tetrazolium assay.…”

Section: In Vitro Anticancer Activitymentioning

confidence: 99%

Biological evaluation, DNA/protein‐binding aptitude of novel dibenzosuberene appended palladium (II)‐thiourea complexes

Rohini

Konakanchi

Aneesrahman

et al. 2018

Applied Organom Chemis

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Four novel Pd (II)-thiourea square-planar derivatives of the type [PdCl 2 L 2 ] (L = N-dibenzosuberenyl appended aroyl/acylthiourea ligand) (1-4) were synthesized and well characterized. The neutral monodentate S coordination of ligand with palladium (II) ion was confirmed by single crystal X-ray diffraction method. In vitro calf thymus DNA (CT-DNA) interaction studies were investigated, and the results of the complexes showed the intercalative mode of binding (in the order of 1 > 3 > 2 > 4) with complexes. The binding affinity of the compounds with bovine serum albumin (BSA) was studied, and it was found that the binding affinity of the complexes with DNA and BSA was different. In addition, the antimicrobial activity evaluation results revealed that compound 2 has registered excellent antibacterial activity against four strains, and also compounds 2 and 4 have shown good antifungal activity against the microorganisms compared with their reference drugs. The scavenging activity of compound 3 showed potent activity with an IC 50 value of 6.95 ± 1.81 μM. Furthermore, the in vitro anti-proliferative activity results clearly suggested that complex 3 showed most potent cytotoxic activity against human neuroblastoma (IMR-32) cell line, with an IC 50 value of 15.21 ± 0.359 μM compared with the reference drug.

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“…Following our ongoing researches in the field of design and preparation of cyclopalladated complexes with antiproliferative activities, we report here the synthesis and investigation of two new Pd complexes of the type [Pd(CN)(NHC)Br] containing a NHC ligand with a pending pyrene unit and two different cyclometalated amino‐benzyl (CN) ligands. The new complexes were characterized using NMR and FT‐IR spectroscopy and thermogravimetric analysis (TGA).…”

Section: Introductionmentioning

confidence: 99%

Luminescent Palladacycles Containing a Pyrene Chromophor; Synthesis, Biological and Computational Studies of the Interaction with DNA and BSA

et al. 2019

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One of the most active areas within the field of bioorganometallic chemistry, complexes of N-heterocyclic carbenes (NHCs), have recently gained interest. Herein, we report two luminescent palladium N-heterocyclic carbene complexes; namely [Pd {(C,N)-C 6 H 4 CH 2 NH(CH 2 CH 3 )}(1)] (2) and [Pd{(C,N)-C 6 H 4 CH 2 NH 2 }(1)] (3) (1 = 1-methyl-3-(2-oxo-2-(pyren-1-yl)ethyl)-2,3-dihydroimidazol-2-ylidene) which were synthesized from the reaction of luminescent imidazolium salt (1(H)Br) and binuclear Palladacycles. The interactions of them with CT-DNA evaluated via absorption, emission and CD spectral techniques as well as measurements of viscosity and thermal denaturation and the results have been shown that they bounded to CT-DNA by intercalation and groove binding modes. The in vitro cytotox-icity of compounds 2-3 and 1(H)Br on human breast (MCF-7) and cervical epithelial carcinoma (HeLa) cancer cells lines, indicated the wide range of anticancer activities of them with low IC 50 values. Moreover, based on the protein binding ability studies, the intrinsic fluorescence of BSA could be strongly quenched by compounds via a static quenching mechanism. Competitive binding study using Eosin, Digoxin and Ibuprofen as site markers, indicated that the compounds could bind to sites I and II on BSA structure. Finally, all data obtained from biophysical studies were validated by molecular modeling study. Computational results showed that palladium complexes have the potential for detection of mismatch DNA.

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Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Cited by 23 publications

References 68 publications

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand

Biological evaluation, DNA/protein‐binding aptitude of novel dibenzosuberene appended palladium (II)‐thiourea complexes

Luminescent Palladacycles Containing a Pyrene Chromophor; Synthesis, Biological and Computational Studies of the Interaction with DNA and BSA

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Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Cited by 23 publications

References 68 publications

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N2O2 donor Schiff base ligand

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N2O2 donor Schiff base ligand

Biological evaluation, DNA/protein‐binding aptitude of novel dibenzosuberene appended palladium (II)‐thiourea complexes

Luminescent Palladacycles Containing a Pyrene Chromophor; Synthesis, Biological and Computational Studies of the Interaction with DNA and BSA

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Product

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Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand

Synthesis and multi‐spectroscopic study on DNA‐binding, cleavage and biological properties of M(II) complexes based on N₂O₂ donor Schiff base ligand