“…Initially, the safety properties of precursor, ligands, and Cu(II) complexes were assessed by toxicity assay, as previously described. [21] In brief, age-synchronized young adult worms (n = 100 worms per condition) were transferred to 1 mL of M9 buffer (5 g NaCl, 3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , 1 mL of 1 M MgSO 4, and 1 L distilled H 2 O) containing 5 mg/mL heat-killed E. coli OP50 and different pharmacological doses of precursor, ligands, and metal complexes (5,10,15,20,25, and 30 μM) at 20°C in 24-well microtiter plates. After 24 h, the worms were observed for inactivity/death under an inverted microscope using the touch-provoked method.…”