Dinuclear 1D coordination polymer of [NaCu2(pdc)2(H2O)4(OH)]n (1) (H2pdc = Pyridine‐2,6‐dicarboxylic acid) was synthesized by the solvothermal method, and its structure was investigated by single‐crystal X‐ray diffraction analysis. 1 has zigzag chains, which are converted to a 3D network through hydrogen bond interactions. Also, 1 was synthesized by sonochemical method (1‐Sono). The structures of 1 and 1‐Sono were characterized by FT‐IR, XRD, FE‐SEM and thermogravimetric (TG) techniques. Antibacterial activity was evaluated by agar well diffusion method. Release of copper ions, chelation, and stability were the factors influencing antibacterial property. More effect of compounds against S. aureus strain was observed, and 1‐Sono exhibited better antibacterial activity than 1.