Synthetic aminomethylnaphthoquinones inhibit the in vitro replication of bovine herpesvirus 5

Abstract

Bovine herpesvirus type 5 (BoHV-5) is an etiologic agent of meningoencephalitis in cattle. The aim of this study was to evaluate the antiviral potential of a series of synthetic Mannich bases derived from lawsone and to investigate at which stage of the BoHV-5 replicative cycle the compounds might be acting. The most potent and selective inhibitor exhibited CC50 and EC50 values of 1867 μM ± 8.3 and 3.8 μM ± 1.2, respectively (ACV: 989 μM ± 2 and 166 μM ± 2, respectively).

Anti-Herpetic, Anti-Dengue and Antineoplastic Activities of Simple and Heterocycle-Fused Derivatives of Terpenyl-1,4-Naphthoquinone and 1,4-Anthraquinone

“…The antiviral activity of several quinones against herpesviruses in late stages of infection has been reported [29,30,31]. In this study, we have found a different kind of antiviral effect of quinones, showing that NQ 4 exerts anti-HHV-1 activity in early stages, specifically avoiding viral attachment.…”

“…In recent years, antiviral activity has been described for certain quinones and other structurally related molecules with therapeutic potential. NQs and AQs have shown antiviral activities against DNA and RNA viruses by inhibition of viral entry, replication and genome transcription, as well as affecting the function of important enzymes for the viral replicative cycle [27,28,29,30,31,32,33]. Emodin, a 9,10-anthraquinone isolated from the roots of Rheum tanguticum , showed in vitro and in vivo anti-herpetic activity affecting viral replication, i.e., in late stages of infectious cycle [15].…”

Antiviral Activity of Diterpenes From Canistrocarpus Cervicornis against Human Herpesvirus 1 and Bovine Herpesvirus 5

“…An antiviral screening test was performed in Vero and MDBK cells grown in 24 well microplates were inoculated with 200 P.F.U of the HHV-1KOS and BoHV-5RJ42/01 respectively as described previously ( 24) with some modifications (28). After one hour for virus adsorption at 37 °C with a 5% CO 2 atmosphere the inoculum was discarded for addition of the differents concentration of diterpenes 1, 2 and ACV (12.5, 25, 50 and 100 µM).…”

“…Virion attachment assay was performed as described earlier [24] with some modifications [28]. Vero and MDBK cells monolayers grown in 24 well microplates were pre-chilled at 4°C for 1 h. The medium was aspirated and a set of the monolayers were inoculated with 200 P.F.U of HHV-1 KOS or BoHV-5RJ42/01 after pre-treatment with compounds (12.5 and 50 µM) for 1 h at 4ºC.…”

“…The HHV-1 KOS and BoHV-5RJ42/01 internalization assay in Vero or MDBK cells was conducted as described Cheng et al 2004 with some modifications [28]. Vero and MDBK cell were grown in 24-well plates for 24 h and pre-chilled at 4 °C for 1 h. Vero or MDBK cell monolayers were inoculated with 200 PFU of HHV-1 KOS and BoHV-5RJ42/01 respectively and maintained at 4°C for 3 h for virus attachment.…”