Synthetic bacterial consortia transplantation for the treatment of Gardnerella vaginalis-induced bacterial vaginosis in mice

Li, Yunxia; Zhu, Wei; Jiang, Yan; Lessing, Duncan James; Chu, Weihua

doi:10.1186/s40168-023-01497-y

Cited by 18 publications

(11 citation statements)

References 50 publications

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“…Numerous studies have investigated the association between vaginal microbes and vaginal atrophy. They have used VMT to treat vaginal microecological imbalances [19]. However, there is a lack of re-search on the role of vaginal microbes in improving vaginal atrophy.…”

Section: Discussionmentioning

confidence: 99%

Vaginal microbiota transplantation alleviates vaginal atrophy in ovariectomized mice

Xu,

Zhu,

Zou

et al. 2024

Preprint

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Genitourinary menopausal syndrome (GSM) is a prevalent symptom in menopausal women, affecting over 50% of older women and patients with loss of ovarian function. The role of factors other than estrogen, such as the vaginal microbiota, in the development of GSM has not been fully explored. Therefore, we selected 8-week-old C57 mice with bilateral oophorectomy for experimentation. After four weeks of treatment, we observed that the vaginal epithelium of ovariectomized mice showed signs of atrophy, and the structure and metabolites of vaginal microbiota showed significant differences. Vaginal transplantation of microbiota from ovary-intact mice significantly ameliorated the vaginal atrophy of ovariectomized mice and altered the structure and metabolism of vaginal microbiota. These findings indicate that ovarian activity significantly affects the structure and metabolism of vaginal microbiota. The vaginal microbiota of ovary-intact mice may promote vaginal health by upregulating ER (estrogen receptor) in vaginal epithelial cells in ovariectomized mice, which in turn promotes cell proliferation. Further studies are needed to investigate the interactions between vaginal bacterial microbiota and vaginal health. This finding can help develop new therapeutic strategies and interventions for patients suffering from vaginal atrophy.

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Section: Discussionmentioning

confidence: 99%

Vaginal microbiota transplantation alleviates vaginal atrophy in ovariectomized mice

Xu,

Zhu,

Zou

et al. 2024

Preprint

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“…The overall microbial community composition was more complex in SUI group. Microbial dysbiosis was observed [23,24] (Figure 2B). Based on the proportion of samples with dysbiosis of the vaginal microbiota in two groups (whether the vaginal ora is dominated by Lactobacillus).…”

Section: The Composition and Biomarkers Of The Vaginal Microbiotamentioning

confidence: 99%

Effect of Stress Urinary Incontinence on Vaginal Microbiota Stability

Zhang,

Zhou,

Yao

et al. 2023

Preprint

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Background Stress urinary incontinence and vaginal microbial dysbiosis frequently occur in postpartum women. A better understanding of the relationship between SUI and the composition of vaginal microbiota may help to prevent the emergence of vaginal diseases. However, limited research has been conducted to investigate the potential association between vaginal microbial dysbiosis and stress urinary incontinence. Results The present study revealed a strong correlation between changes in the composition of vaginal microbiota and stress urinary incontinence, with stress urinary incontinence being more likely to lead to dysbiosis of vaginal microbiota. In terms of the vaginal microbiota, both alpha diversity and evenness were significantly higher in the stress urinary incontinence group compared to the group without stress urinary incontinence. Additionally, there were notable differences in the composition of the vaginal microbiota between two groups. Specifically, among stress urinary incontinence patients, the abundance of bacteria associated with bacterial vaginosis, such as Streptococcus, Prevotella, Dialister, and Veillonella, showed an increase, while the abundance of Lactobacillus decreased. Furthermore, the vaginal microbial co-occurrence network of stress urinary incontinence patients displayed higher connectivity, complexity, and clustering. Conclusions As a result, our study emphasizes the significant role of Lactobacillus in maintaining homeostasis within the vaginal microbial community. A correlation between stress urinary incontinence and the vaginal microbiota was observed, indicating an increased risk of vaginal microbial disorders and bacterial vaginosis for individuals with stress urinary incontinence. Consequently, we anticipate that our findings will contribute novel insights into the prevention of bacterial vaginal diseases associated with stress urinary incontinence.

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“…The findings revealed that SBCT effectively mitigated vaginal tissue damage, rebalanced the vaginal microbiota, and decreased the release of pro-inflammatory cytokines like IL-1b and IL-8. Furthermore, SBCT inhibited NF-jB activation and reduced the expression of IL-17, iNOS, and COX-2 in vaginal tissues [87]. Tilapia piscidin 4 (TP4)-an antimicrobial peptide from Nile tilapia-has a broad antibacterial activity, its potential role in modulating macrophage response during BV was not understood [88].…”

Section: Bv Treatment Prognosis: Insights From Cytokine Dynamicsmentioning

confidence: 99%

Inflammatory mediators in bacterial vaginosis: The role of cytokines

Zhang,

2024

APMIS

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BV is a significant concern in women's health with a varying prevalence rate in different cities of China. The condition has been linked to the acquisition of STIs, including HIV and HPV, and can lead to infertility, adverse obstetric outcomes. We conducted a comprehensive literature search in the PubMed. The search was performed from 01/01/2018 to 01/09/2023. The following search terms were used: bacterial vaginosis and cytokine. We also manually searched the reference lists of included studies and relevant reviews to identify additional articles. The presence of Gardnerella spp. can lead to changes in cytokine levels. The immune system of the female reproductive tract consists of various immune cells and molecules that play a vital role in defending against infections. Cytokines, signaling molecules involved in immune cell recruitment and activation, have been identified as potential biomarkers for diagnosing BV and predicting STIs. Current treatments for BV primarily involve antibiotics, but there is a high recurrence rate posttreatment. BV is a complex condition that affects a significant number of women worldwide. The role of cytokines in the onset, progression, and treatment of BV offers promising avenues for future research and potential diagnostic and therapeutic advancements.

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Synthetic bacterial consortia transplantation for the treatment of Gardnerella vaginalis-induced bacterial vaginosis in mice

Cited by 18 publications

References 50 publications

Vaginal microbiota transplantation alleviates vaginal atrophy in ovariectomized mice

Vaginal microbiota transplantation alleviates vaginal atrophy in ovariectomized mice

Effect of Stress Urinary Incontinence on Vaginal Microbiota Stability

Inflammatory mediators in bacterial vaginosis: The role of cytokines

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