Synthetic PEG Hydrogels as Extracellular Matrix Mimics for Tissue Engineering Applications

Papavasiliou, Georgia; Sokic, Sonja; Turturro, Michael V.

doi:10.5772/31695

Cited by 17 publications

(12 citation statements)

References 76 publications

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“…Few studies investigate both the effects of 3D scaffolds and cyclic tensile strain on hMSC differentiation without osteogenic supplements. In the few studies that do not use osteogenic media, most use scaffolds or coatings comprised of collagen, which is a major component in bone and hence provides a biological factor that induces its own cell response [ 57 , 58 ].…”

Section: The Effect Of Cyclic Tensile Strain On Mscsmentioning

confidence: 99%

Strain and Vibration in Mesenchymal Stem Cells

McClarren

Olabisi

2018

International Journal of Biomaterials

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Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into any mesenchymal tissue, including bone, cartilage, muscle, and fat. MSC differentiation can be influenced by a variety of stimuli, including environmental and mechanical stimulation, scaffold physical properties, or applied loads. Numerous studies have evaluated the effects of vibration or cyclic tensile strain on MSCs towards developing a mechanically based method of differentiation, but there is no consensus between studies and each investigation uses different culture conditions, which also influence MSC fate. Here we present an overview of the response of MSCs to vibration and cyclic tension, focusing on the effect of various culture conditions and strain or vibration parameters. Our review reveals that scaffold type (e.g., natural versus synthetic; 2D versus 3D) can influence cell response to vibration and strain to the same degree as loading parameters. Hence, in the efforts to use mechanical loading as a reliable method to differentiate cells, scaffold selection is as important as method of loading.

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Section: The Effect Of Cyclic Tensile Strain On Mscsmentioning

confidence: 99%

Strain and Vibration in Mesenchymal Stem Cells

McClarren

Olabisi

2018

International Journal of Biomaterials

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“…Oocyte quality may be improved by longer maturation periods (> 7 days), or modulation of the hydrogel (e.g. alginate, fibrin) (Papavasiliou et al, 2012; Shikanov et al, 2011b) or transplant environment. Heterotopic sites offer advantages regarding ease of access, yet may lack the natural cues present at orthotopic sites.…”

Section: Discussionmentioning

confidence: 99%

“…This result may suggest that oocyte quality can be improved to facilitate progression to a blastocyst stage for embryo transfer. Oocyte quality may be improved by longer maturation periods (>7 days), or modulation of the hydrogel (e.g., alginate and fibrin; Papavasiliou, Sokic, & Turturro, 2012; or transplant environment. Heterotopic sites offer advantages regarding ease of access, yet may lack the natural cues present at orthotopic sites.…”

Section: Discussionmentioning

confidence: 99%

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility preservation as an important consideration. Among fertility preservation strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only clinical option available to cancer survivors with cryopreserved tissue. However, follicle atresia after transplantation and risk of reintroducing malignant cells have prevented this procedure from becoming widely adopted in clinics. Herein, we investigated the encapsulation of ovarian follicles in alginate hydrogels that isolate the graft from the host, yet allows for maturation after transplantation at a heterotopic (i.e., subcutaneous) site, a process we termed in vivo follicle maturation. Survival of multiple follicle populations was confirmed via histology, with the notable development of the antral follicles. Collected oocytes (63%) exhibited polar body extrusion and were fertilized by intracytoplasmic sperm injection and standard in vitro fertilization procedures. Successfully fertilized oocytes developed to the pronucleus (14%), two-cell (36%), and four-cell (7%) stages. Furthermore, ovarian follicles cotransplanted with metastatic breast cancer cells within the hydrogels allowed for retrieval of the follicles, and no mice developed tumors after removal of the implant, confirming that the hydrogel prevented seeding of disease within the host. Collectively, these findings demonstrate a viable option for safe use of potentially cancer-laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection. Ultimately, this technology may provide novel options to preserve fertility for young female patients with cancer.

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“…x 10 -6 (cm 2 s -1 ) by Lawrence et al [30] and Xu et al [31]. It is well-known that PEG does not allow protein adsorption [32,33], and PDGF-BB has a low affinity for fibrin within the porous structure [34,35]. Based on this information, we assumed that PDGF-BB did not interact with the scaffold.…”

Section: Diffusion Modelingmentioning

confidence: 94%

Biomaterials with persistent growth factor gradients in vivo accelerate vascularized tissue formation

Akar¹,

Jiang²,

Somo³

et al. 2015

Biomaterials

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Gradients of soluble factors play an important role in many biological processes, including blood vessel assembly. Gradients can be studied in detail in vitro, but methods that enable the study of spatially distributed soluble factors and multi-cellular processes in vivo are limited. Here, we report on a method for the generation of persistent in vivo gradients of growth factors in a three-dimensional (3D) biomaterial system. Fibrin loaded porous poly (ethylene glycol) (PEG) scaffolds were generated using a particulate leaching method. Platelet derived growth factor BB (PDGF-BB) was encapsulated into poly (lactic-co-glycolic acid) (PLGA) microspheres which were placed distal to the tissue-material interface. PLGA provides sustained release of PDGF-BB and its diffusion through the porous structure results in gradient formation. Gradients within the scaffold were confirmed in vivo using near-infrared fluorescence imaging and gradients were present for more than 3 weeks. The diffusion of PDGF-BB was modeled and verified with in vivo imaging findings. The depth of tissue invasion and density of blood vessels formed in response to the biomaterial increased with magnitude of the gradient. This biomaterial system allows for generation of sustained growth factor gradients for the study of tissue response to gradients in vivo.

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Synthetic PEG Hydrogels as Extracellular Matrix Mimics for Tissue Engineering Applications

Cited by 17 publications

References 76 publications

Strain and Vibration in Mesenchymal Stem Cells

Strain and Vibration in Mesenchymal Stem Cells

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Biomaterials with persistent growth factor gradients in vivo accelerate vascularized tissue formation

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