Synthetic Thermoreversible Polymers Are Compatible with Osteoinductive Activity of Recombinant Human Bone Morphogenetic Protein 2

Gao, Tiejun; Kousinioris, Niki; Wozney, John M.; Winn, Shelley R.; Uludağ, Hasan

doi:10.1089/107632702760184691

Cited by 27 publications

(22 citation statements)

References 21 publications

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“…The ability of synthetic polymers to induce osteogenic cell differentiation is on the other hand generally lower than that of ceramics, unless growth factors are incorporated and released in a controlled fashion [71].…”

Section: Three-dimensional Scaffoldsmentioning

confidence: 99%

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Bone and cartilage tissue engineering for facial reconstructive surgery

Farhadi

Jaquiéry

Haug

et al. 2006

IEEE Eng. Med. Biol. Mag.

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Section: Three-dimensional Scaffoldsmentioning

confidence: 99%

“…(iii) Specific growth factors could also be incorporated within a polymer scaffold which, by degradation, will release the factor with defined kinetics [75].…”

Section: Growth Factorsmentioning

confidence: 99%

Bone and cartilage tissue engineering for facial reconstructive surgery

Farhadi

Jaquiéry

Haug

et al. 2006

IEEE Eng. Med. Biol. Mag.

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“…PEMA bone cements have been investigated as an alternative to PMMA due to their higher ductility, lower toxicity, and exotherm, 34 water absorptiondesorption characteristics, and interactions with the biological medium studied. 35 PEMA derivates have been studied for the preparation of intervertebral cages 36 and as carriers for the recombinant human bone morphogenetic protein 2 37 and fosfosal. 38 The production of composites based on the in situ polymerization of EMA and VP with several amounts of dispersed W-Tc is presented in this article.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, bioactivity and biocompatibility of nanostructured materials based on the wollastonite‐poly(ethylmethacrylate‐co‐vinylpyrrolidone) system

Rodríguez‐Lorenzo

Carrodeguas

Rodrı́guez

et al. 2008

J Biomedical Materials Res

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Composite materials are very promising biomaterials for hard tissue augmentation. The approach assayed in this work involves the manufacturing of a composite made of a bioactive ceramic, natural wollastonite (W) and a nanostructured copolymer of ethylmethacrylate (EMA) and vinylpyrrolidone (VP) to yield a bioresorbable and biocompatible VP-EMA copolymer. A bulk polymerization was induced thermally at 50 degrees C, using 1 wt % azobis(isobutyronitrile) (AIBN) as free-radical initiator. Structural characterization, compressive strength, flexural strength (FS), degradation, bioactivity, and biocompatibility were evaluated in specimens with a 60/40 VP/EMA ratio and ceramic content in the range 0-60%. A good integration between phases was achieved. Greater compression and FS, in comparison with the pure copolymer specimens was obtained only when the ceramic load got up to 60% of the total weight. The soaking in NaCl solution resulted in the initial swelling of the specimens tested. The maximum swelling was reached after 2-3 h of immersion and it was significantly greater for lower ceramic loads. This result makes the polymer component the main responsible for the interactions with the media. After soaking in SBF, microdomains segregation can be observed in the polymer component that can be related with a dramatic difference in the reactivity of both monomers in free radical polymerization, whereas the formation of an apatite-like layer on the W surfaces can be observed. Biocompatibility in vitro studies showed the absence of cytotoxicity of all formulations. The cells were able to adhere on the polystyrene negative control and on specimens containing 60 wt % wollastonite forming a monolayer and showing a normal morphology. However, a low cellular growth was observed.

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“…If these requirements of carrier are satisfied, predictable bone regeneration is possible with sufficient value to the tissue engineering side. There have been many rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue studies on carriers [14][15][16][17] , but no carriers have satisfied these requirements perfectly.…”

Section: Introductionmentioning

confidence: 99%

rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue

Kim

Yun

Chae

et al. 2008

J Korean Acad Periodontol

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Purpose: The carrier for the delivery of bone morphogenetic proteins(BMPs) should also serve as a scaffold for new bone growth. In addition, predictable bone formation in terms of the volume and shape should be guaranteed. This study evaluated the ectopic bone formation of recombinant human BMP-2(rhBMP-2) using a micro macroporous biphasic calcium phosphate (MBCP: mixture of β-TCP and HA) block as a carrier in a rat subcutaneous assay model. Materials and Methods: Subcutaneous pockets were created on the back of 40 male Sprague-Dawley rats. In the pockets, rhBMP-2/MBCP and MBCP alone were implanted. The blocks were evaluated by histological and histometric parameters after a healing interval of 2 weeks (each 10 rats; MBCP and rhBMP-2/MBCP) or 8 weeks (each 10 rats; MBCP and rhBMP-2/MBCP). Results: The shape and volume of the block was maintained stable over the healing period. No histological bone forming activity was observed in the MBCP alone sites after 2 weeks and there was minimal new bone formation at 8 weeks. In the rhBMP-2/MBCP sites, new bone formation was evident in the macropores of the block. The new bone area at 8 weeks was greater than at 2 weeks. There was a further increase in the quantity of new bone with the more advanced stage of remodeling. Conclusions: A MBCP block could serve as a carrier system for predictable bone tissue engineering using rhBMPs. KEY WORDS: rhBMP-2; biphasic calcium phosphate block; carrier; ectopic bone formation.

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Synthetic Thermoreversible Polymers Are Compatible with Osteoinductive Activity of Recombinant Human Bone Morphogenetic Protein 2

Cited by 27 publications

References 21 publications

Bone and cartilage tissue engineering for facial reconstructive surgery

Bone and cartilage tissue engineering for facial reconstructive surgery

Synthesis, characterization, bioactivity and biocompatibility of nanostructured materials based on the wollastonite‐poly(ethylmethacrylate‐co‐vinylpyrrolidone) system

rhBMP-2 using biphasic calcium phosphate block as a carrier induces new bone formation in a rat subcutaneous tissue

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