SYP72 interacts with the mechanosensitive channel MSL8 to protect pollen from hypoosmotic shock during hydration

Zhou, Xuemei; Zheng, Yifan; Wang, Ling; Li, Haiming; Guo, Yingying; Li, Mengdi; Sun, Mingwei; Zhao, Peng

doi:10.1038/s41467-021-27757-9

Cited by 10 publications

(5 citation statements)

References 30 publications

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“…Complementation of the sweet13; sweet14 mutant phenotype by SWEET13 N76Q and SWEET13 S142N was tested; wild-type SWEET13 served as a positive control. Pollen viability was evaluated by double staining with fluorescent diacetate (FDA; stains viable pollen with intact cell membranes) and propidium iodide (PI; stains pollen with compromised cell membrane) (20). In wild-type Arabidopsis , 97% of pollen grains were FDA positive, but only 8% of sweet13; sweet14 pollen grains were stained (Fig.…”

Section: Resultsmentioning

confidence: 99%

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SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

Isoda¹,

Palmai²,

Yoshinari³

et al. 2022

Preprint

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SWEET sucrose transporters play important roles in the allocation of sucrose in plants. Some SWEETs were shown to also mediate transport of the plant growth regulator gibberellin (GA). The close physiological relationship between sucrose and GA raised the questions of if there is a functional connection, and whether one or both of the substrates are physiologically relevant. To dissect these two activities, molecular dynamics were used to map the binding sites of sucrose and GA in the pore of SWEET13 and predicted binding interactions that might be selective for sucrose or GA. Transport assays confirmed these predictions. In transport assays, the N76Q mutant had 7x higher relative GA3 activity, and the S142N mutant only transported sucrose. The impaired pollen viability and germination in sweet13;14 double mutants were complemented by the sucrose-selective SWEET13S142N but not by the SWEET13N76Q mutant, indicating that sucrose is the physiologically relevant substrate and that GA transport capacity is dispensable in the context of male fertility. Therefore, GA supplementation to counter male sterility may act indirectly via stimulating sucrose supply in male sterile mutants. These findings are also relevant in the context of the role of SWEETs in pathogen susceptibility.

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Section: Resultsmentioning

confidence: 99%

“…Propidium iodide-Fluorescent Diacetates (PI-FDA) assays for pollen viability and in vitro pollen germination assays were performed as described (20, 39). Experimental details are provided in SI Appendix, SI Text .…”

Section: Methodsmentioning

confidence: 99%

SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

Isoda¹,

Palmai²,

Yoshinari³

et al. 2022

Preprint

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show abstract

“…Previously, syntaxin proteins are found to regulate cell tip growth in bacteria, fungi and model plant species Arabidopsis . For examples, in yeast, syntaxin Tlg1p protein mediates polarized growth by mediating delivery of chitin synthase III (Holthuis et al ., 1998); in N. crassa , syntaxin NSYN1/2 proteins are involved in hyphal tip growth by regulating vesicle fusion (Gupta et al ., 2003); in Arabidopsis , syntaxin of plant SYP121, SYP123, SYP132, and AtSYP81 proteins promote root tip growth by mediating vesicle fusion (Hirano et al ., 2023; Wang et al ., 2023), SYP124 is involved in pollen tube tip growth (Silva et al ., 2010), SYP1s and SYP72 plays a key role in pollen development and germination (Li et al ., 2019; Zhou et al ., 2022). To our knowledge, we are the first to identify the role of syntaxin protein in polarized cell growth in important crop plants (i.e.…”

Section: Discussionmentioning

confidence: 99%

PbrSYP71regulates pollen tube growth by maintaining uneven distribution of the ERin Pyrus

Zhang,

Tang,

Lan

et al. 2024

Preprint

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The pollen tube secretion system provides essential substances to support polarized pollen tube growth. The endoplasmic reticulum (ER) is a crucial component of the secretory system, and is predominantly concentrated in the pollen tube subapical region. However, the mechanism underlying the regulation of ER accumulation in pollen tube remains unclear. In this study, we identified a syntaxin protein PbrSYP71 that promoted pollen tube growth by regulating ER aggregation at pollen tube tip in pear. We found that PbrSYP71 anchored ER via the transmembrane domain. Reducing PbrSYP71 expression decreased the accumulation of ER in pollen tube. Moreover, overexpression of PbrSYP71 resulted in bundle-like aggregation of the ER in tobacco leaf cells. Such aggregation state was disappeared when cytoplasmic domain of PbrSYP71 protein was absent or actin cytoskeleton was depolymerized. Additionally, the ER accumulation was decreased when the actin filament was depolymerized in pear pollen tube. We conclude that PbrSYP71 positively regulates ER accumulation in the subapical area of pollen tube by anchoring ER to actin, providing new insights into the underlying mechanisms of ER accumulation during polarized cell growth.

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“…The pollen viability was observed by Alexander staining as described [ 60 ], with slight modifications. The anthers were fixed overnight in Carnoy’s fixative solution (anhydrous ethanol: glacial acetic acid, 3:1).…”

Section: Methodsmentioning

confidence: 99%

“…Aniline blue staining was carried out to explore pollen germination according to previous descriptions [ 60 ], with slight modifications. After 24 h of hand pollination, pistils were fixed overnight in Carnoy’s fixative solution.…”

Section: Methodsmentioning

confidence: 99%

The Histone Variant H3.3 Is Required for Plant Growth and Fertility in Arabidopsis

Long,

Yang,

et al. 2024

IJMS

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Histones are the core components of the eukaryote chromosome, and have been implicated in transcriptional gene regulation. There are three major isoforms of histone H3 in Arabidopsis. Studies have shown that the H3.3 variant is pivotal in modulating nucleosome structure and gene transcription. However, the function of H3.3 during development remains to be further investigated in plants. In this study, we disrupted all three H3.3 genes in Arabidopsis. Two triple mutants, h3.3cr-4 and h3.3cr-5, were created by the CRISPR/Cas9 system. The mutant plants displayed smaller rosettes and decreased fertility. The stunted growth of h3.3cr-4 may result from reduced expression of cell cycle regulators. The shorter stamen filaments, but not the fertile ability of the gametophytes, resulted in reduced fertility of h3.3cr-4. The transcriptome analysis suggested that the reduced filament elongation of h3.3cr-4 was probably caused by the ectopic expression of several JASMONATE-ZIM DOMAIN (JAZ) genes, which are the key repressors of the signaling pathway of the phytohormone jasmonic acid (JA). These observations suggest that the histone variant H3.3 promotes plant growth, including rosette growth and filament elongation.

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SYP72 interacts with the mechanosensitive channel MSL8 to protect pollen from hypoosmotic shock during hydration

Cited by 10 publications

References 30 publications

SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

PbrSYP71regulates pollen tube growth by maintaining uneven distribution of the ERin Pyrus

The Histone Variant H3.3 Is Required for Plant Growth and Fertility in Arabidopsis

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