Systematic analysis of in vitro photo-cytotoxic activity in extracts from terrestrial plants in Peninsula Malaysia for photodynamic therapy

Ong, Cheng Yi; Ling, Sui Kiong; Ali, Rasadah Mat; Chee, Chin Fei; Samah, Zainon Abu; Ho, Anthony Siong Hock; Teo, Soo Hwang; Lee, Hong Boon

doi:10.1016/j.jphotobiol.2009.06.009

Cited by 61 publications

(38 citation statements)

References 14 publications

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“…Upconversion nanoparticle-mediated PDT induces ThP-1 macrophage apoptosis [25][26][27] have demonstrated that PDT induces both apoptosis and necrosis depending on the drug concentrations as well as the laser irradiation time and intensity. In this study, we evaluated the type of cell death that occurred following UCNPs-Ce6-mediated PDT using three different methods.…”

mentioning

confidence: 99%

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Zhu¹,

Wang²,

Zheng³

et al. 2015

IJN

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Atherosclerosis (AS) is the most vital cardiovascular disease, which poses a great threat to human health. Macrophages play an important role in the progression of AS. Photodynamic therapy (PDT) has emerged as a useful therapeutic modality not only in the treatment of cancer but also in the treatment of AS. The purpose of this study was to determine the molecular mechanisms underlying the activity of PDT, using mesoporous-silica-coated upconversion fluorescent nanoparticles encapsulating chlorin e6 (UCNPs-Ce6) in the induction of apoptosis in THP-1 macrophages. Here, we investigated the ability of UCNPs-Ce6-mediated PDT to induce THP-1 macrophage apoptosis by facilitating the induction of reactive oxygen species (ROS) and regulation of mitochondrial permeability transition pore (MPTP) to depolarize mitochondrial membrane potential (MMP). Both Bax translocation and the release of cytochrome C were examined using immunofluorescence and Western blotting. Our results indicated that the levels of ROS were significantly increased in the PDT group, resulting in both MPTP opening and MMP depolarization, which led to apoptosis. In addition, immunofluorescence and Western blotting revealed that PDT induced both Bax translocation and the release of cytochrome C, as well as upregulation of cleaved caspase-9, cleaved caspase-3, and cleaved poly(ADP-ribose) polymerase. Therefore, we demonstrated that UCNPs-Ce6-mediated PDT induces apoptosis in THP-1 macrophages via ROS bursts. The proapoptotic factor Bax subsequently translocates from the cytosol to the mitochondria, resulting in the MPTP opening and cytochrome C release. This study demonstrated the great potential of UCNPs-Ce6-mediated PDT in the treatment of AS.

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confidence: 99%

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Zhu¹,

Wang²,

Zheng³

et al. 2015

IJN

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“…This could imply that root part of G. atroviridis can be further explored for anticancer potential. Meanwhile, leaves extract of this species inhibit promyelocytic leukemia (HL60) cell line when tested in photodynamic therapy with combination of MTT assay (25). Compound 9 and 10 isolated from G. atroviridis fruit were also not cytotoxic against human T-lymphoblastic leukaemia (CeM-SS) and human B-lymphoblastoid (raji) cell line as well as not toxic in brine shrimp lethality assay (26).…”

Section: Cytotoxic Activitymentioning

confidence: 99%

Garcinia atroviridis – A review on phytochemicals and pharmacological properties

Taher¹,

Hamidon²,

Susanti³

et al. 2016

Marmara Pharmaceutical Journal

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“…In Malaysia, F. deltoidea is sold in the form of dried or powdered leaves, stems, roots or fruits, as an ingredient in bottled drinks or mixed with other beverages such as coffee (Musa, 2006). Among others, F. deltoidea contains flavonoid, coumarin, alkaloid, steroid, triterpene and salicylic acid (Ong et al, 2009). The plant possesses stronger antioxidant activity than Trolox and vitamin C (Oh et al, 2011).…”

Section: Mangosteen: a 'Fruity' Depigmenting Agentmentioning

confidence: 99%

Development of Natural Cosmeceuticals: Harnessing Asia's Biodiversity

Aziz

Hamid

Noor

et al. 2014

Novel Plant Bioresources

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Systematic analysis of in vitro photo-cytotoxic activity in extracts from terrestrial plants in Peninsula Malaysia for photodynamic therapy

Cited by 61 publications

References 14 publications

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Garcinia atroviridis – A review on phytochemicals and pharmacological properties

Development of Natural Cosmeceuticals: Harnessing Asia's Biodiversity

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