Systematic analysis of modulating activities of native human urinary Tamm-Horsfall protein on calcium oxalate crystallization, growth, aggregation, crystal-cell adhesion and invasion through extracellular matrix

Noonin, Chadanat; Peerapen, Paleerath; Yoodee, Sunisa; Kapincharanon, Chompunoot; Kanlaya, Rattiyaporn; Thongboonkerd, Visith

doi:10.1016/j.cbi.2022.109879

Cited by 23 publications

(12 citation statements)

References 61 publications

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“…How to cite this article: Noonin, C., Peerapen, P., & Thongboonkerd, V. (2022). Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study.…”

Section: S U P P O R T I N G I N F O R M At I O Nmentioning

confidence: 99%

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Noonin

Peerapen

Thongboonkerd

2022

J of Extracellular Bio

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Bacterial overgrowth is common for improperly stored urine. However, its effects on human urinary extracellular vesicles (uEVs) study had not been previously examined nor documented. This study investigated the presence of bacterial EVs (bEVs) contaminated in uEVs samples and their effects on uEVs study. Nanoscale uEVs were isolated from normal human urine immediately after collection (0-h) or after 25 • Cstorage with/without preservative (10 mM NaN 3 ) for up to 24-h. Turbidity, bacterial count and total uEVs proteins abnormally increased in the 8-h and 24-h-stored urine without NaN 3 . NanoLC-ESI-LTQ-Orbitrap MS/MS identified 6-13 bacterial proteins in these contaminated uEVs samples. PCR also detected bacterial DNAs in these contaminated uEVs samples. Besides, uEVs derived from 8-h and 24-h urine without NaN 3 induced macrophage activation (CD11b and phagocytosis) and secretion of cytokines (IFN-α, IL-8, and TGF-β) from macrophages and renal cells (HEK-293, HK-2, and MDCK). All of these effects induced by bacterial contamination were partially/completely prevented by NaN 3 . Interestingly, macrophage activation and cytokine secretion were also induced by bEVs purified from Escherichia coli. This study clearly shows evidence of bEVs contamination and their effects on human uEVs study when the urine samples were inappropriately stored, whereas NaN 3 can partially/completely prevent such effects from the contaminated bEVs.

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Section: S U P P O R T I N G I N F O R M At I O Nmentioning

confidence: 99%

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Noonin

Peerapen

Thongboonkerd

2022

J of Extracellular Bio

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“…The interference effect of urinary THP on the adhesion of other ions on the surface of calcium oxalate crystal has been studied previously; a reduction in the maximum negative potential on the crystal surface was found to promote crystal aggregation. When the concentration of urinary THP increases, the effect of THP on the promotion of crystal aggregation is more obvious; this effect was strengthened as the ionic strength increased [9,10]. Related studies showed that crystallization in the kidney also increased and that the expression levels of THP increased signi cantly [11].…”

Section: Discussionmentioning

confidence: 99%

“…In healthy individuals, a small amount of THP will be discharged into the urine. When kidney injury is caused by various reasons such as obstruction, in ammation, or autoimmune diseases, the content of THP in the urine will increase; the greater the degree of kidney injury, the higher the concentration of THP in the urine [8,9]. THP has dual effects on the growth and aggregation of calcium oxalate crystals; these processes can be affected by many factors [5].…”

Section: Discussionmentioning

confidence: 99%

Predictive value of urinary Tamm-Horsfall protein and 24-hour urine osmolality for recurrence in patients with calcium oxalate stones

Fang¹,

Tang²,

Wang³

et al. 2023

Preprint

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Introduction: To investigate the predictive value of urinary Tamm-Horsfall protein (THP) and 24-h urine osmolality for recurrence in patients with calcium oxalate (CaOx) stones. Methods: The clinical data of 120 patients hospitalized for upper urinary tract stones between January 2020 and January 2022 in our hospital were retrospectively reviewed. Patients with and without stone recurrence at postoperative follow-up were divided into a recurrence group (53 patients) and a non-recurrence group (67 patients); 50 healthy patients during the same period were selected as the control group. Levels of urinary THP, 24-h urine osmolality, and biochemical indices were then compared between the three groups. Receiver operating characteristic (ROC) curves were applied to predict the predictive values of urinary THP and 24-h urine osmolality for stone recurrence. Results: Urinary THP, 24-hour urine osmolality, and biochemical indices in the recurrence group were significantly higher than those in the non-recurrence group and the control group (P < 0.05). 24-h urine osmolality was positively correlated with urinary oxalic acid and calcium excretion. ROC curve analysis showed that the optimal cut-off values for urinary THP and 24-h urine osmolality for the prediction of stone development were ≥27.01mg/L and ≥577.69mOsm/(kg·H2O); furthermore, the combination of these indices significantly improved the accuracy of prediction. Conclusion: Urinary THP and 24-hour urine osmolality are higher in patients with recurrent CaOx stones; the combined detection of these parameters can accurately predict stone recurrence.

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“…Under some particular circumstances, this protein can promote crystal aggregation [ 109 ]. However, several in vitro and in vivo studies have reported that uromodulin serves as a potent inhibitor of calcium stones [ 110 , 111 ]. Therefore, lime powder prevents KSD by increasing urinary concentrations of citrate and uromodulin, both of which are the potent inhibitors of calcium stone formation.…”

Section: Strategies To Prevent Ksdmentioning

confidence: 99%

Kidney Stone Prevention

Peerapen¹,

Thongboonkerd²

2023

Advances in Nutrition

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Systematic analysis of modulating activities of native human urinary Tamm-Horsfall protein on calcium oxalate crystallization, growth, aggregation, crystal-cell adhesion and invasion through extracellular matrix

Cited by 23 publications

References 61 publications

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Predictive value of urinary Tamm-Horsfall protein and 24-hour urine osmolality for recurrence in patients with calcium oxalate stones

Kidney Stone Prevention

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