Systematic, computer-assisted development of high performance liquid chromatography for multi-component analysis

Abstract

Based on the QbD concept, a new HPLC-DAD/ELSD method was established to determine multiple components in the TCM preparation with the cooperation of modeling software Drylab and statistical software JMP.

Key information related to quality by design (QbD) applications in analytical methods development

“…Sun et al [126] combined a computer simulation program and a design of experiments statistical software to develop a multicomponent analytical determination method using HPLC, combining two detectors, a diode-array detector (DAD) and an evaporative light scattering detector (ELSD), connected in series. DoE was applied to in the workflow steps to minimize experimental risk variables and provide an efficient DS method.…”

Metrics for green analytical chemistry

“…As an example, a generic liquid chromatographic method with sample preparation based on extraction will be investigated. 10 Fig. 1 presents the Ishikawa diagram of the factors inuencing the performance of the method.…”

“…Ishikawa diagram showing the factors influencing the analytical performance of a generic LC method. Modified from Sun et al, (2015) 10. …”

Development of an analytical method by defining a design space: a case study of saponin determination for Panax notoginseng extracts

“…2,9,10 Many analytical methods were developed using the design space approach successfully. 11,12 However, the optimization of several HPLC gradients in a method was rarely involved. A possible reason is that the addition of gradients will result in a remarkable increase of analytical parameters.…”