Systematic modulating carbon metabolism to improve α-farnesene production in Pichia pastoris

Xu, Jianzhong; Chen, Sheng-Ling; Niu, Si-Qi

doi:10.1016/j.fuproc.2023.107757

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Notably, 13,14-Dihydro-15-keto prostaglandin A2, which has high discriminatory power, is a potential biomarker for sepsis, associated with increased morbidity and mortality risk [54]. Similarly, alpha-farnesene, the main alarm pheromone, has potential applications as a protective agent in agriculture [55]. Stercobilin, found in the urine and feces of many mammals, including humans, serves as an indicator of fecal contamination in environmental water [56].…”

Section: Discussionmentioning

confidence: 99%

Regulatory Effects of Ganoderma lucidum, Grifola frondosa, and American ginseng Extract Formulation on Gut Microbiota and Fecal Metabolomics in Mice

Zhang,

Huang,

Zhao

2023

Foods

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The bioactivities of Ganoderma lucidum, Grifola frondosa, and American ginseng have been extensively studied and documented. However, the effects of their complexes on the structural properties of intestinal microbiota and fecal metabolism remain unclear. Therefore, this paper aims to present a preliminary study to shed light on this aspect. In this study, an immunocompromised mouse model was induced using cyclophosphamide, and Ganoderma lucidum, Grifola frondosa, and American ginseng extract formulation (referred to as JGGA) were administered via gavage to investigate their modulatory effects on gut microbiota and fecal metabolism in mice. The effects of JGGA on immune enhancement were explored using serum test kits, hematoxylin–eosin staining, 16SrDNA high-throughput sequencing, and UHPLC-QE-MS metabolomics. The findings revealed potential mechanisms underlying the immune-enhancing effects of JGGA. Specifically, JGGA administration resulted in an improved body weight, thymic index, splenic index, carbon scavenging ability, hypersensitivity, and cellular inflammatory factor expression levels in mice. Further analysis demonstrated that JGGA reduced the abundance of Firmicutes, Proteobacteria, and Actinobacteria, while increasing the abundance of Bacteroidetes. Additionally, JGGA modulated the levels of 30 fecal metabolites. These results suggest that the immune enhancement observed with JGGA may be attributed to the targeted modulation of gut microbiota and fecal metabolism, thus promoting increased immunity in the body.

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Section: Discussionmentioning

confidence: 99%