Systematic protocol for assessment of the validity of BOLD MRI in a rabbit model of inflammatory arthritis at 1.5 tesla

Chan, Michael W.Y.; Nathanael, George; Kis, Antonella; Amirabadi, Afsaneh; Zhong, Anguo; Rayner, Tammy; Weiss, Ruth; Detzler, Garry; Jong, Roland; Gahunia, Harpal K.; Moineddin, Rahim; Crawley, Adrian P.; Doria, A.

doi:10.1007/s00247-013-2844-5

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…These methods have inherent shortcomings, which are mainly uncertainty and cost. For animal experiments, most investigators have resorted to euthanizing animals to obtain results on local disease status 27,28. Ultrasound‐guided biopsy in patients has been accepted as a minimally invasive and safe approach, which is well tolerated by patients while enabling the collection of high‐quality synovial tissues.…”

Section: Discussionmentioning

confidence: 99%

“…For animal experiments, most investigators have resorted to euthanizing animals to obtain results on local disease status. 27,28 Ultrasound-guided biopsy in patients has been accepted as a minimally invasive and safe approach, which is well tolerated by patients while enabling the collection of high-quality synovial tissues. This study has shown that this insight can also be extrapolated to experimental RA in rabbits, which should allow more preclinical longitudinal studies in the future.…”

Section: Discussionmentioning

confidence: 99%

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Sonography Is Superior to Serum‐Based Biomarkers for Measuring Disease Status in Experimental Rheumatoid Arthritis

Chen

Zheng

Liu

et al. 2016

J of Ultrasound Medicine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sonography Is Superior to Serum‐Based Biomarkers for Measuring Disease Status in Experimental Rheumatoid Arthritis

Chen

Zheng

Liu

et al. 2016

J of Ultrasound Medicine

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“…Development and application of new technologies that can identify and evaluate these pathological biomarkers, namely neoangiogenesis, hyperemia, and hypoxia, may shed new light on research and clinical management of inflammatory arthritis. Magnetic resonance imaging (MRI) has been used to identify increased vascularity related to inflammatory arthritis and assess the hemoglobin oxygenation in arthritis with a rabbit model 6 , 7 . However, the high cost and controlled access can limit the widespread use of MRI for general clinical screening and diagnosis.…”

Section: Introductionmentioning

confidence: 99%

A Functional Study of Human Inflammatory Arthritis Using Photoacoustic Imaging

Cao

et al. 2017

Sci Rep

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By using our dual-modality system enabling simultaneous real-time ultrasound (US) and photoacoustic (PA) imaging of human peripheral joints, we explored the potential contribution of PA imaging modality to rheumatology clinic. By performing PA imaging at a single laser wavelength, the spatially distributed hemoglobin content reflecting the hyperemia in synovial tissue in metacarpophalangeal (MCP) joints of 16 patients were imaged, and compared to the results from 16 healthy controls. In addition, by performing PA imaging at two laser wavelengths, the spatially distributed hemoglobin oxygenation reflecting the hypoxia in inflammatory joints of 10 patients were imaged, and compared to the results from 10 healthy controls. The statistical analyses of the PA imaging results demonstrated significant differences (p < 0.001) in quantified hemoglobin content and oxygenation between the unequivocally arthritic joints and the normal joints. Increased hyperemia and increased hypoxia, two important physiological biomarkers of synovitis reflecting the increased metabolic demand and the relatively inadequate oxygen delivery in affected synovium, can both be objectively and non-invasively evaluated by PA imaging. The proposed dual-modality system has the potential of providing additional diagnostic information over the traditional US imaging approaches and introducing novel imaging biomarkers for diagnosis and treatment evaluation of inflammatory arthritis.

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“…57,58 The proliferative and invasive nature of arthritis synovium has frequently been compared to tumor development, both of them exhibit the apparent paradoxical features of hypoxia and angiogenesis. 59 The space of rabbit's knee joints was narrow, thus the periarticular aspect of the knee rather than the synovial microvascular response was evaluated.…”

mentioning

confidence: 99%

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

Guo¹,

Xu²,

He³

et al. 2023

JPR

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Knee osteoarthritis (KOA) is a chronic inflammatory disease highly associated with intra-articular hypertension, hypoxia and angiogenesis of synovial tissue. Our previous studies showed that acupotomy could treat KOA in a variety of ways, including reducing cartilage deterioration and enhancing biomechanical qualities. However, the mechanism of hypoxia and angiogenesis induced by acupotomy in KOA synovium remains unclear. This study looked for the benign intervention of acupotomy in synovial pathology. Methods: The rabbits were divided into 3 groups, Normal group, KOA group, and KOA + Acupotomy (Apo) group, with 11 rabbits in each group. The KOA rabbit model was established by the modified Videman method with six weeks. The KOA + Apo group performed the intervention. The tendon insertion of vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa were selected as treatment points in rabbits. Rabbits were treated once every 7 days for 3 weeks. We observed the intra-articular pressure and oxygen partial pressure (BOLD MRI). The synovial morphology was monitored by Hematoxylin-Eosin Staining (HE Staining). The expression of hypoxia-inducible transcription factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) was detected using Immunohistochemical (IHC), Western Blot and Enzyme-Linked Immunosorbent Assay (ELISA). Results: Acupotomy reduced intra-articular hypertension and improved the synovial oxygen situation, synovial inflammatory and angiogenesis. HIF-1α, VEGF, IL-1β and TNF-α expression were downregulated by acupotomy. Conclusion: Acupotomy may reduce inflammation and angiogenesis in KOA rabbit by reducing abnormally elevated intra-articular pressure and improving synovial oxygen environment. The above may provide a new theoretical foundation for acupotomy treatment of KOA.

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Systematic protocol for assessment of the validity of BOLD MRI in a rabbit model of inflammatory arthritis at 1.5 tesla

Abstract: The most clinically relevant reading parameters included a lower threshold of r > 0.01 and a positive + negative BOLD response. We propose that diff_on_off is a more clinically relevant summary measure of BOLD MRI, while PT% can be used as an ancillary measure.

Cited by 4 publications

References 48 publications

Sonography Is Superior to Serum‐Based Biomarkers for Measuring Disease Status in Experimental Rheumatoid Arthritis

Sonography Is Superior to Serum‐Based Biomarkers for Measuring Disease Status in Experimental Rheumatoid Arthritis

A Functional Study of Human Inflammatory Arthritis Using Photoacoustic Imaging

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

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