Systematic Toxicological Analysis Revealing a Rare Case of Captan Ingestion

Gottzein, Anne Kathrin; Mußhoff, Frank; Madea, Burkhard

doi:10.1111/1556-4029.12154

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…In the pretreatment step of biological samples, the previous studies have used mainly LLE or direct injection after protein precipitation . The direct injected samples after protein precipitation are mainly used for LC analysis and they usually show high matrix effect and significant signal suppression .…”

Section: Resultsmentioning

confidence: 99%

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A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: Application for therapeutic drug monitoring

Νικολάου

Papoutsis

Spiliopoulou

et al. 2014

J of Separation Science

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A simple gas chromatographic method with mass spectrometry detection was developed and validated for the determination of lacosamide in human plasma. Lacosamide and the internal standard, levetiracetam-d6, were extracted from 200 μL plasma, by a solid-phase extraction through HF Bond Elut C18 columns, and derivatized using N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide with 1% tert-butyldimethylsilylchloride in acetonitrile. The limit of quantification was found to be 0.20 μg/mL and the assay was linear up to 20.0 μg/mL with correlation coefficient ≥0.994. The intra- and interday precision values were <4.1% in terms of relative standard deviation (%) and the values of intra- and interday accuracy were found to be within -7.2 and 5.3% in terms of relative error (%). Absolute recovery of the method for lacosamide was determined at three concentration levels and ranged from 92.5 to 97.6%. The developed method uses small volumes of plasma and proved to be simple, rapid, and sensitive for the determination of lacosamide in plasma. This method can be used in routine every day analysis of plasma samples obtained from patients who follow respective antiepileptic treatment and for the investigation of clinical and forensic cases where lacosamide is involved.

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Section: Resultsmentioning

confidence: 99%

“…All published methods for the determination of lacosamide in biological samples use LC . To our knowledge, there is no validated method in the literature for the determination of lacosamide using GC–MS.…”

Section: Introductionmentioning

confidence: 99%

A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: Application for therapeutic drug monitoring

Νικολάου

Papoutsis

Spiliopoulou

et al. 2014

J of Separation Science

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“…Table 1 lists examples of recently published STA strategies. These usually involve various stages of sample preparation followed by a variety of drug screening and quantification methods 9,26–28 …”

Section: Outline Of Sta Strategies In Forensic Toxicologymentioning

confidence: 99%

Overview of systematic toxicological analysis strategies and their coverage of substances in forensic toxicology

Rygaard

Pan

Nielsen

et al. 2023

Analytical Science Advances

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Systematic toxicological analysis (STA) is the process of using an adequate analytical methodology to detect and identify as many potentially toxicologically relevant compounds as possible in biological samples. STA is an important part of everyday routine work within forensic toxicology, and several methods for STA have frequently been published and reviewed independently. However, the many drugs and other substances involved, as well as the constant emergence of new ones, may pose a major challenge in STA, which often demands a strategy involving multiple analytical methods in parallel. Such strategies have been published and evaluated less frequently despite their relevance in forensic toxicology. This mini‐review briefly summarizes commonly applied methods for STA in forensic toxicology, including gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–MS (LC–MS) methods, and highlights some of their potential pitfalls. Second, it provides an overview of previously reported strategies to conduct STA, including a presentation of the STA strategy applied in the authors’ laboratory. This involves broad drug screening by LC–high‐resolution MS, supported by targeted screening and quantification using LC–tandem MS, headspace (HS)‐GC–MS, HS‐GC–flame ionization detector and other complementary methods. The STA strategy aims to cover as many potentially relevant drugs as possible and seeks to reduce potential pitfalls arising in forensic casework. The review underlines that not every substance can be identified in all circumstances even with a comprehensive STA strategy.

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“…This SPME was successfully applied to detect thrombin in human plasma [19]. When SPME was used to screen for VOCs, the results suggested a possible cause of death from a rare case of captan ingestion [90]. With convenient sample preparation [91], SPME could be a valuable tool for the early diagnosis of cardiovascular, oncologic and neurodegenerative illnesses.…”

Section: Spme Applications For Metabolite Analysismentioning

confidence: 99%

Solid-phase microextraction technology for in vitro and in vivo metabolite analysis

Zhang

Zhou

Chen

et al. 2016

TrAC Trends in Analytical Chemistry

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Analysis of endogenous metabolites in biological samples may lead to the identification of biomarkers in metabolomics studies. To achieve accurate sample analysis, a combined method of continuous quick sampling and extraction is required for online compound detection. Solid-phase microextraction (SPME) integrates sampling, extraction and concentration into a single solvent-free step for chemical analysis. SPME has a number of advantages, including simplicity, high sensitivity and a relatively non-invasive nature. In this article, we reviewed SPME technology in in vitro and in vivo analyses of metabolites after the ingestion of herbal medicines, foods and pharmaceutical agents. The metabolites of microorganisms in dietary supplements and in the gastrointestinal tract will also be examined. As a promising technology in biomedical and pharmaceutical research, SPME and its future applications will depend on advances in analytical technologies and material science.

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Systematic Toxicological Analysis Revealing a Rare Case of Captan Ingestion

Cited by 11 publications

References 16 publications

A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: Application for therapeutic drug monitoring

A fully validated method for the determination of lacosamide in human plasma using gas chromatography with mass spectrometry: Application for therapeutic drug monitoring

Overview of systematic toxicological analysis strategies and their coverage of substances in forensic toxicology

Solid-phase microextraction technology for in vitro and in vivo metabolite analysis

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