Systemic and mucosal immune responses in red tilapia (Oreochromis sp.) following immersion vaccination with a chitosan polymer-based nanovaccine against Aeromonas veronii

Sukkarun, Pimwarang; Kitiyodom, Sirikorn; Kamble, Manoj Tukaram; Bunnoy, Anurak; Boonanuntanasarn, Surintorn; Yata, Teerapong; Boonrungsiman, Suwimon; Thompson, Kim D.; Rodkhum, Channarong; Pirarat, Nopadon

doi:10.1016/j.fsi.2024.109383

Cited by 2 publications

(1 citation statement)

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“…A turbidimetric method was employed for the quantification of serum lysozyme (LYZ) levels [ 36 ]. In 96-well U-bottom microtiter plates, 15 µL of test serum was mixed with 150 µL of Micrococcus lysodeikticus (0.3 mg/mL in 0.02 M of a sodium acetate buffer, pH 5.5).…”

Section: Methodsmentioning

confidence: 99%

Effect of Moringa oleifera Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae Biotype 2

Kamble,

Gallardo,

Salin

et al. 2024

Animals

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The present study aimed to investigate the effects of Moringa oleifera leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against Streptococcus agalactiae Biotype 2. Four hundred and fifty Nile tilapia (32.61 ± 0.2 g/fish) were randomly allocated into fifteen tanks (30 fish/tank). Different concentrations of MLE at 0%, 0.5%, 1%, 1.5%, and 2% were fed to the Nile tilapia for 30 days, and the growth, feed utilization, hematology, and innate immune response of the Nile tilapia were determined. After the feeding trial, the Nile tilapia were challenged with a S. agalactiae Biotype 2 infection, and the relative percentage of survival (RPS) was determined. Results revealed the presence of quercetin, kaempferol, and p-coumaric acid in the MLE extract, exhibiting stronger antimicrobial activity against S. agalactiae Biotype 2. The diets supplemented with the MLE-0.5 group showed a significantly higher growth, feed utilization, hematology, and innate immune response in the Nile tilapia compared to the control and other MLE groups. Additionally, the MLE-0.5 group exhibited a significantly higher RPS of the Nile tilapia against S. agalactiae Biotype 2. Therefore, MLE-0.5 can be employed as an alternative feed supplement in sustainable Nile tilapia farming to protect against S. agalactiae Biotype 2.

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Section: Methodsmentioning

confidence: 99%