Systemic lymphoproliferative responses to rotavirus

Totterdell, B.M.; Banatvala, J. E.; Chrystie, I. L.; Ball, G.; Cubitt, W. D.

doi:10.1002/jmv.1890250106

Cited by 26 publications

(20 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…rubella [34]. The high prevalence of rotavirus IgM seen in a previous study [19] was also explained by possible persistence of antibody although this was not apparent in other studies of infants [31] or adults [20].…”

Section: Discussionmentioning

confidence: 79%

“…Studies which have investigated rotaviral infection in older age groups have long suggested that infection rates could be high, especially in case contacts [14,15]. Adult rotaviral infection has been measured using viral antigen in stools [16,17] and through serology using IgM as a marker of recent or current infection [18][19][20][21]. However, these studies did not consider seasonal variation which is normally considerable in most geographical settings except the tropics [22].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serological survey of anti-group A rotavirus IgM in UK adults

Cox

Medley

2003

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYRotaviral associated disease of infants in the UK is seasonal and infection in adults not uncommon but the relationship between these has been little explored. Adult sera collected monthly for one year from routine hospital samples were screened for the presence of anti-group A rotavirus immunoglobulin M class antibodies as a marker of recent infection. Anti-rotavirus IgM was seen in all age groups throughout the year with little obvious seasonal variation in the distribution of antibody levels. IgM concentrations and the proportion seropositive above a threshold both increased with age with high concentrations consistently observed in the elderly. Results suggest either high infection rates of rotavirus in adults, irrespective of seasonal disease incidence in infants, IgM persistence or IgM cross-reactivity. These results support recent evidence of differences between infant and adult rotavirus epidemiology and highlight the need for more extensive surveys to investigate age and time related infection and transmission of rotavirus.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Serological survey of anti-group A rotavirus IgM in UK adults

Cox

Medley

2003

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…In 1988, Totterdell et al [9] were the first to utilize a lymphoproliferative assay to test T‐cell responses to rotavirus, when they observed cellular responses to rotavirus in various groups of adults. Offit et al.…”

Section: Introductionmentioning

confidence: 99%

Rotavirus-specific T-cell responses in young prospectively followed-up children

Mäkelä

Marttila

Simell

et al. 2004

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYRotavirus is a major cause of gastroenteritis in young children. Antibodies seem to protect against rotavirus infection but cell-mediated immune responses are probably also important for protection. We evaluated the development of T-cell responses to rotavirus in follow-up samples from 20 healthy children with an increased genetic risk for type 1 diabetes. Blood samples from 16 healthy adults were also available for the study. T-cell proliferation was analysed at 3-6 month intervals from the age of 3 months to the age of 4-5 years using the Wa strain of human rotavirus and the NCDV strain of bovine rotavirus as antigens. IgG and IgA antibodies to rotavirus were studied from simultaneously drawn plasma samples with EIA method using NCDV as an antigen. A total of 24 infections were revealed by antibody analysis. Sixteen children showed diagnostic increases in both IgG and IgA antibodies to rotavirus, while 5 children showed increases in IgA antibodies only and 3 in IgG only. Antibody rises were accompanied by T-cell responses to rotavirus (SI > 3) in 9 of the 24 cases. T-cell responses to purified or lysed human rotavirus were stronger after a rise in rotavirus antibodies than the responses before infection ( P = 0·017 and 0·027, respectively). There was a correlation between Tcell responses to purified and lysed human rotavirus and NCDV. Strong T-cell responses to rotavirus were transient and the ability to respond usually disappeared in one year, but in all adults T-cell responses to rotavirus were strong implicating that several infections are needed to develop consistent, strong T-cell responsiveness.

show abstract

“…In spite of the fact that the importance of humoral immune response in gastroenteric viral infections of porcine is well recognized (Corthesy and Kraehenbuhl, 1999;Saif et al, 1994;Tô et al, 1998;Van Cott et al, 1994;Yuan et al, 1996), little is known about cell-mediated immunity, particularly in PEDV infections. However, it has been proposed that cellular immune response may play an important role in the protection and recovery from infection, besides the fact that production of antibodies is regulated by cytokines produced by activated T lymphocytes and other mononuclear cells (Corthesy and Kraehenbuhl, 1999;Kraehenbuhl and Neutra, 1992;Saif, 1999;Totterdell et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Lymphoproliferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus

Arriba

Carvajal

Pozo

et al. 2002

Journal of Virological Methods

View full text Add to dashboard Cite

Lymphocyte proliferative responses were evaluated in mucosal (mesenteric lymph nodes) and systemic (spleen and blood) lymphoid tissues of conventional piglets inoculated with the virulent or attenuated isolates of porcine epidemic diarrhoea virus (PEDV) strain CV-777 and challenged 21 days later with the virulent isolate of the same virus. A lymphoproliferative assay was developed in which mononuclear cells isolated from lymphoid tissues at different postinoculation and postchallenge days underwent a secondary in vitro stimulation with semipurified antigen obtained from PEDV-infected cell cultures. Vigorous lymphocyte proliferative responses were detected in the pigs inoculated with the virulent PEDV at postinoculation days 4-21, especially in the mesenteric lymph nodes and the blood; however, in the spleen this response was lower and less regular. The pigs inoculated with the attenuated virus showed a less intense response, the higher lymphocyte proliferation also corresponded to the mononuclear cells from mesenteric lymph nodes. Lymphocyte proliferation responses showed high correlations with protection against homologous challenge with virulent PEDV, and this correlation was higher in the gut associated lymphoid tissues (mesenteric lymph nodes). The cell proliferation response detected in blood mirrored that detected in the mesenteric lymph nodes, and showed also good correlation with protection. The results confirm that T-cell-helper function, assessed by lymphocyte proliferation responses, contributes to establishing a protective immune response against PEDV infections.

show abstract

Systemic lymphoproliferative responses to rotavirus

Cited by 26 publications

References 14 publications

Serological survey of anti-group A rotavirus IgM in UK adults

Serological survey of anti-group A rotavirus IgM in UK adults

Rotavirus-specific T-cell responses in young prospectively followed-up children

Lymphoproliferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus

Contact Info

Product

Resources

About