Systemic modulation of peripheral eosinophilia (air pouch model) in Schistosoma mansoni infection

Pacheco, RG

doi:10.1590/s0074-02761997000800022

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…Air pouches have been used to great effect in analyzing inflammatory responses induced by a wide range of materials, including bacteria and protozoan parasites. A similar technique has also been used to assess inflammatory responses induced by eggs from the helminth Schistosoma mansoni (28). The work described above would appear to be the first published report of the use of the air pouch technique to study the inflammatory response induced by a skin-invasive stage of a helminth infection.…”

Section: Discussionmentioning

confidence: 99%

Trapping and Immobilization ofNippostrongylus brasiliensisLarvae at the Site of Inoculation in Primary Infections of Interleukin-5 Transgenic Mice

1999

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Interleukin-5 (IL-5) transgenic mice are highly resistant to primary infections with the intestinal nematode Nippostrongylus brasiliensis; few parasites are found in the intestines of infected animals, and egg production is minimal. While adult worms may be damaged in the intestine, larval migration, development, and viability may also be impaired in other tissues. This study addresses the migration of N. brasiliensis larvae through the skin and lungs and associated cellular responses in primary infections of IL-5 transgenic mice. Although some larvae may have been trapped and killed in the lungs of IL-5 transgenic mice, most apparently failed to reach this site. Two or more hours after infection of IL-5 transgenic mice, eosinophils were a major component of the cellular infiltrate at the subcutaneous site of injection, and localized eosinophil degranulation was extensive. Seventy-five to ninety-five percent of the larvae injected into subcutaneous air pouches in IL-5 transgenic mice were retained there for at least 24 h. In contrast, in nontransgenic mice, less than 20% of larvae could be recovered from the skin 2 or more h postinjection, and eosinophil activity was modest at all times. The data strongly suggest that eosinophils can restrict the movement of N. brasiliensis larvae in the first few hours of a primary infection and that this has profound effects on later stages of parasite development. Preexisting eosinophilia, due either to allergy or to infection with tissue-invasive helminth species, may therefore confer some degree of immediate and nonspecific resistance in primary infections with parasitic worms.

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Section: Discussionmentioning

confidence: 99%

Trapping and Immobilization ofNippostrongylus brasiliensisLarvae at the Site of Inoculation in Primary Infections of Interleukin-5 Transgenic Mice

1999

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“…Air pouches have been used with superb results in the analysis of inflammatory responses induced by a wide range of materials, including bacteria (Castro et al, 1991;Clark and Weinhold, 1979;Tessier et al, 1998), protozoa (Matte and Olivier, 2002;Pacheco and Lenzi, 1997) and other parasites (Daly et al, 1999;Bower et al, 2008), but this model had not been previously tested for T. crassiceps infection.…”

Section: Introductionmentioning

confidence: 99%

A mouse air pouch model for evaluating the immune response to Taenia crassiceps infection

Gaspar

Sakai

Gaspari

2014

Experimental Parasitology

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The experimental system of Taenia crassiceps cysticerci infection in BALB/c mice is considered to be the most representative model of cysticercosis. In our work, mice were sacrificed 7 and 30days after infection, and pouch fluid was collected to determine the number of accumulated cells and the concentrations of IFNγ, IL-2, IL-4, IL-6, IL-10 and nitric oxide. The injection of 50 nonbudding cysticerci into normal mouse dorsal air pouches induced a high level of IFNγ and nitric oxide production relative to the parasite load. The air pouch provides a convenient cavity that allows studying the cellular immunological aspects of the T. crassiceps parasite. The nonbudding cysticerci recovered from the air pouches contained cells that can reconstitute complete cysts in the peritoneal cavity of mice. In conclusion, these results demonstrate that the air pouch model is an alternative tool for the evaluation of the immune characteristics of T. crassiceps infection.

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“…It is well established that an important step in the extravasation of eosinophils is their adhesion to the vascular endothelium, which is mediated by selectins and chemokines (Lampinen et al 2004). However, the acute eosinophil cellular response against eggs and its contribution to granuloma formation is not well studied (Pacheco & Lenzi 1997). Furthermore, there are no reports in the literature showing alteration in the plasma concentration of chemokines in acutely infected patients.…”

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confidence: 99%

Seric chemokines and chemokine receptors in eosinophils during acute human schistosomiasis mansoni

Silveira-Lemos

Teixeira-Carvalho²,

Martins‐Filho³

et al. 2010

Mem. Inst. Oswaldo Cruz

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The recruitment of circulating eosinophils by chemokines and chemokine receptors plays an important role in the inflammation process in acute human schistosomiasis. Our main focus has been on the plasma chemokines (CXCL8/CCL2/CCL3/CCL24) and chemokine receptors (CCR2/CCR3/CCR5/CXCR1/CXCR2/CXCR3/CXCR4) expressed by circulating eosinophils from acute Schistosoma mansoni infected patients (ACT). Our studies compared ACT patients and healthy individuals as a control group. Our major findings demonstrated a plethora of chemokine secretion with significantly increased secretion of all chemokines analysed in the ACT group. Although no differences were detected for beta-chemokine receptors (CCR2, CCR3 and CCR5) or alpha-chemokine receptors (CXCR3 and CXCR4), a significantly lower frequency of CXCR1+ and CXCR2+ eosinophils in the ACT group was observed. The association between chemokines and their chemokine receptors revealed that acutely infected schistosome patients displaying decreased plasma levels of CCL24 are the same patients who presented enhanced secretion of CCL3, as well as increased expression of both the CCR5 and CXCR3 chemokine receptors. These findings suggest that CCL24 may influence the kinetics of chemokines and their receptors and eosinophils recruitment during human acute schistosomiasis mansoni

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Systemic modulation of peripheral eosinophilia (air pouch model) in Schistosoma mansoni infection

Abstract: Schistosoma mansoni infection induces in their hosts

Cited by 3 publications

References 36 publications

Trapping and Immobilization ofNippostrongylus brasiliensisLarvae at the Site of Inoculation in Primary Infections of Interleukin-5 Transgenic Mice

Trapping and Immobilization ofNippostrongylus brasiliensisLarvae at the Site of Inoculation in Primary Infections of Interleukin-5 Transgenic Mice

A mouse air pouch model for evaluating the immune response to Taenia crassiceps infection

Seric chemokines and chemokine receptors in eosinophils during acute human schistosomiasis mansoni

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