Systemic ß adrenergic stimulation/ sympathetic nerve system stimulation influences intraocular RAS through cAMP in the RPE

Martins, Joana Raquel; Reichhart, Nadine; Kociok, Nobert; Stindl, Julia; Foeckler, Renate; Lachmann, P. J.; Todorov, Vladimir; Castrop, Hayo; Strauß, Olaf

doi:10.1016/j.exer.2019.107828

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…Epinephrine, an endogenous molecule that can be used to treat cardiac arrest and anaphylaxis in a hospital environment, may also influence retinal physiology. Systemic adrenergic stimulation with isoproterenol, a β1-and β2-adrenergic receptors agonist, impact RPE renin expression, with implications on retinal pathophysiology [ 136 ]. The influence of stimulating the adrenergic system in the retina is not only related to changes in the renin–angiotensin system, but the stimulation can also affect RPE ion transport.…”

Section: Drugs and Medicinementioning

confidence: 99%

Retinal Toxicity Induced by Chemical Agents

Araújo

Brito

Pereira-Figueiredo

et al. 2022

IJMS

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Vision is an important sense for humans, and visual impairment/blindness has a huge impact in daily life. The retina is a nervous tissue that is essential for visual processing since it possesses light sensors (photoreceptors) and performs a pre-processing of visual information. Thus, retinal cell dysfunction or degeneration affects visual ability and several general aspects of the day-to-day of a person’s lives. The retina has a blood–retinal barrier, which protects the tissue from a wide range of molecules or microorganisms. However, several agents, coming from systemic pathways, reach the retina and influence its function and survival. Pesticides are still used worldwide for agriculture, contaminating food with substances that could reach the retina. Natural products have also been used for therapeutic purposes and are another group of substances that can get to the retina. Finally, a wide number of medicines administered for different diseases can also affect the retina. The present review aimed to gather recent information about the hazard of these products to the retina, which could be used to encourage the search for more healthy, suitable, or less risky agents.

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Section: Drugs and Medicinementioning

confidence: 99%

Retinal Toxicity Induced by Chemical Agents

Araújo

Brito

Pereira-Figueiredo

et al. 2022

IJMS

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“…9,11 It has been known for some time that adrenergic receptors are present on RPE cells, 35 and recently it has been shown that there are adrenergic nerve endings in close proximity to the basolateral membrane of the mouse RPE and β1 and β2 adrenergic receptors are present on mouse RPE cells. 36 Unlike what has been found in rabbit, 34 bovine, 9 and human RPE, 10 the effect of epinephrine on the mouse RPE short-circuit current was small, but significant, whereas there was no effect on TER. This is similar to what has been found in the rabbit RPE 34 (Table 2).…”

Section: Ouabainmentioning

confidence: 78%

Mechanisms of Ion Transport Across the Mouse Retinal Pigment Epithelium Measured In Vitro

Skarphedinsdottir

Eysteinsson

Árnason

2020

Invest. Ophthalmol. Vis. Sci.

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To examine ion transport across the mouse retinal pigment epithelium (RPE), measured by the short-circuit current (I SC) and transepithelial resistance (TER). METHODS. Sheets of RPE from mice (C57BL6/J) with retina, choroid, and sclera attached were mounted in Ussing chambers (0.031-cm 2 aperture) and Krebs solution. The I SC and TER were recorded with voltage clamps. Receptors implicated in ion transport were blocked or stimulated by ligands applied to both sides. RESULTS. The mean initial I SC was −12.0 ± 3.9 μA/cm 2 (basolateral negative), and mean TER was 67.1 ± 8.0 ohm•cm 2. RPE preparations remained stable for 3 hours, with I SC decreasing by 0.078 ± 0,033 μA/cm 2 /hr. Adenosine triphosphate (100 μM) increased I SC by 2.22 ± 0.41 μA/cm 2 (P = 0.003). Epinephrine (100 μM) increased I SC by 1.14 ± 0.19 μA/cm 2 (P = 0.011). Bumetanide (100 μM) reduced I SC by 1.72 ± 0.73 μA/cm 2 (P = 0.027). Ouabain (1 mM) induced a biphasic response: an I SC increase from −7.9 ± 2.4 to −15.49 ± 2.12 μA/cm 2 and then a decrease to −3.7 ± 2.2 μA/cm 2. Ouabain increased TER by 15.3 ± 4.8 ohm•cm 2. These compounds were added sequentially. Apical [K + ] o at zero mM transiently increased I SC by 3.36 ± 1.06 μA/cm 2. Ba ++ decreased I SC from −10.4 ± 3.1 to −6.6 ± 1.8 μA/cm 2 (P = 0.01). Ba ++ reversed the K +-free response, with I sc decreasing further from −5.65 ± 1.24 to −3.37 ± 0.79 μA/cm 2 (P = 0.029). CONCLUSIONS. The I SC and TER can be recorded from the mouse RPE for 3 hours. Adrenergic and purinergic receptors affect murine RPE ion transport. Sodium-potassium adenosine triphosphatase plays a role in net ion transport across mouse RPE, and Na-K-2Cl cotransporter activity partly accounts for transepithelial ion transport. Mimicking lightinduced changes, low subretinal [K + ] o increases ion transport transiently, dependent on K + channels.

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“…However, neurite outgrowth itself is not necessarily a positive factor, as it can be associated with the loss of axon guidance in the retina [ 79 , 80 ]. In addition to inherited diseases and trauma, retinal degeneration can be caused by cAMP-induced increased activity of the renin-angiotensin system (RAS) due to hypertension or diabetic retinopathy [ 81 ].…”

Section: Targets Of Camp Regulationmentioning

confidence: 99%

Multiple Roles of cAMP in Vertebrate Retina

Erofeeva

Meshalkina

Firsov

2023

Cells

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cAMP is a key regulatory molecule that controls many important processes in the retina, including phototransduction, cell development and death, growth of neural processes, intercellular contacts, retinomotor effects, and so forth. The total content of cAMP changes in the retina in a circadian manner following the natural light cycle, but it also shows local and even divergent changes in faster time scales in response to local and transient changes in the light environment. Changes in cAMP might also manifest or cause various pathological processes in virtually all cellular components of the retina. Here we review the current state of knowledge and understanding of the regulatory mechanisms by which cAMP influences the physiological processes that occur in various retinal cells.

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Systemic ß adrenergic stimulation/ sympathetic nerve system stimulation influences intraocular RAS through cAMP in the RPE

Cited by 6 publications

References 28 publications

Retinal Toxicity Induced by Chemical Agents

Retinal Toxicity Induced by Chemical Agents

Mechanisms of Ion Transport Across the Mouse Retinal Pigment Epithelium Measured In Vitro

Multiple Roles of cAMP in Vertebrate Retina

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