Systemic versus tissue-level prolactin signaling in a teleost during a tidal cycle

Seale, André P.; Pavlosky, K. Keano; Celino-Brady, Fritzie T.; Yamaguchi, Yoko; Breves, Jason P.; Lerner, Darren T.

doi:10.1007/s00360-019-01233-9

Cited by 14 publications

(5 citation statements)

References 58 publications

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“…A decrease in prl mRNA levels was shown in black chin tilapia beginning on the first post-experimental day after SW exposure and lasting throughout the SW period, which was restored in FW to baseline control levels [ 62 ]. This is consistent with the observation made in isolated prl cells that fish salinity acclimation history influences osmotic responsiveness [ 63 , 64 , 65 , 66 , 67 ]. Based on the wide range of tissues known to respond to prl across teleosts, it is widely assumed that prl is a conserved regulator of physiological responses to low-salinity environments [ 23 ].…”

Section: Discussionsupporting

confidence: 92%

Effects of Osmotic Stress on the mRNA Expression of prl, prlr, gr, gh, and ghr in the Pituitary and Osmoregulatory Organs of Black Porgy, Acanthopagrus schlegelii

Nagarajan

Aruna

Chang

et al. 2023

IJMS

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In euryhaline teleost black porgy, Acanthopagrus schlegelii, the glucocorticoid receptor (gr), growth hormone receptor (ghr), prolactin (prl)-receptor (prlr), and sodium–potassium ATPase alpha subunit (α-nka) play essential physiological roles in the osmoregulatory organs, including the gill, kidney, and intestine, during osmotic stress. The present study aimed to investigate the impact of pituitary hormones and hormone receptors in the osmoregulatory organs during the transfer from freshwater (FW) to 4 ppt and seawater (SW) and vice versa in black porgy. Quantitative real-time PCR (Q-PCR) was carried out to analyze the transcript levels during salinity and osmoregulatory stress. Increased salinity resulted in decreased transcripts of prl in the pituitary, α-nka and prlr in the gill, and α-nka and prlr in the kidney. Increased salinity caused the increased transcripts of gr in the gill and α-nka in the intestine. Decreased salinity resulted in increased pituitary prl, and increases in α-nka and prlr in the gill, and α-nka, prlr, and ghr in the kidney. Taken together, the present results highlight the involvement of prl, prlr, gh, and ghr in the osmoregulation and osmotic stress in the osmoregulatory organs (gill, intestine, and kidney). Pituitary prl, and gill and intestine prlr are consistently downregulated during the increased salinity stress and vice versa. It is suggested that prl plays a more significant role in osmoregulation than gh in the euryhaline black porgy. Furthermore, the present results highlighted that the gill gr transcript’s role was solely to balance the homeostasis in the black porgy during salinity stress.

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Section: Discussionsupporting

confidence: 92%

Effects of Osmotic Stress on the mRNA Expression of prl, prlr, gr, gh, and ghr in the Pituitary and Osmoregulatory Organs of Black Porgy, Acanthopagrus schlegelii

Nagarajan

Aruna

Chang

et al. 2023

IJMS

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“…The adenohypophyseal hormones Prl and Gh are involved in the physiological adaptation to varying salinities ( 5 , 20 , 49 , 55 , 56 , 58 , 66 , 78 ). In the present study, we observed a decrease in prl mRNA levels in the blackchin tilapia starting from the first post-experimental day after seawater exposure and during the entire seawater period, which back in freshwater raised to baseline control levels.…”

Section: Discussionmentioning

confidence: 99%

Effects of seawater and freshwater challenges on the Gh/Igf system in the saline-tolerant blackchin tilapia (Sarotherodon melanotheron)

et al. 2022

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Prolactin (Prl) and growth hormone (Gh) as well as insulin-like growth factor 1 (Igf1) are involved in the physiological adaptation of fish to varying salinities. The Igfs have been also ascribed other physiological roles during development, growth, reproduction and immune regulation. However, the main emphasis in the investigation of osmoregulatory responses has been the endocrine, liver-derived Igf1 route and local regulation within the liver and osmoregulatory organs. Few studies have focused on the impact of salinity alterations on the Gh/Igf-system within the neuroendocrine and immune systems and particularly in a salinity-tolerant species, such as the blackchin tilapia Sarotherodon melanotheron. This species is tolerant to hypersalinity and saline variations, but it is confronted by severe climate changes in the Saloum inverse estuary. Here we investigated bidirectional effects of increased salinity followed by its decrease on the gene regulation of prl, gh, igf1, igf2, Gh receptor and the tumor-necrosis factor a. A mixed population of sexually mature 14-month old blackchin tilapia adapted to freshwater were first exposed to seawater for one week and then to fresh water for another week. Brain, pituitary, head kidney and spleen were excised at 4 h, 1, 2, 3 and 7 days after both exposures and revealed differential expression patterns. This investigation should give us a better understanding of the role of the Gh/Igf system within the neuroendocrine and immune organs and the impact of bidirectional saline challenges on fish osmoregulation in non-osmoregulatory organs, notably the complex orchestration of growth factors and cytokines.

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“…Euryhaline teleost fish such as Mozambique tilapia, Oreochromis mossambicus , possess the capacity to tolerate a wide range of salinities. Mozambique tilapia have evolved in continually changing environments that are characterized by tidal changes in salinity spanning fresh water (FW) and seawater (SW) and are able to modify the function of the cells and tissues that regulate salt and water balance ( Inokuchi et al, 2015 ; Moorman et al, 2014 ; Seale et al, 2019 ). Prolactin (Prl) is responsible for a variety of physiological actions in vertebrates including fish, amphibians, reptiles, and mammals ( Bern and Nicoll, 1968 ).…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent Decline in Salinity Tolerance in a Euryhaline Fish

et al. 2021

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Euryhaline teleost fish are characterized by their ability to tolerate a wide range of environmental salinities by modifying the function of osmoregulatory cells and tissues. In this study, we experimentally addressed the age-related decline in the sensitivity of osmoregulatory transcripts associated with a transfer from fresh water (FW) to seawater (SW) in the euryhaline teleost, Mozambique tilapia, Oreochromis mossambicus. The survival rates of tilapia transferred from FW to SW were inversely related with age, indicating that older fish require a longer acclimation period during a salinity challenge. The relative expression of Na+/K+/2Cl− cotransporter 1a (nkcc1a), which plays an important role in hyposmoregulation, was significantly upregulated in younger fish after SW transfer, indicating a clear effect of age in the sensitivity of branchial ionocytes. Prolactin (Prl), a hyperosmoregulatory hormone in O. mossambicus, is released in direct response to a fall in extracellular osmolality. Prl cells of 4-month-old tilapia were sensitive to hyposmotic stimuli, while those of >24-month-old fish did not respond. Moreover, the responsiveness of branchial ionocytes to Prl was more robust in younger fish. Taken together, multiple aspects of osmotic homeostasis, from osmoreception to hormonal and environmental control of osmoregulation, declined in older fish. This decline appears to undermine the ability of older fish to survive transfer to hyperosmotic environments.

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Systemic versus tissue-level prolactin signaling in a teleost during a tidal cycle

Cited by 14 publications

References 58 publications

Effects of Osmotic Stress on the mRNA Expression of prl, prlr, gr, gh, and ghr in the Pituitary and Osmoregulatory Organs of Black Porgy, Acanthopagrus schlegelii

Effects of Osmotic Stress on the mRNA Expression of prl, prlr, gr, gh, and ghr in the Pituitary and Osmoregulatory Organs of Black Porgy, Acanthopagrus schlegelii

Effects of seawater and freshwater challenges on the Gh/Igf system in the saline-tolerant blackchin tilapia (Sarotherodon melanotheron)

Age-Dependent Decline in Salinity Tolerance in a Euryhaline Fish

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