T Cell Receptor Vβ Repertoire Expression Reflects Gastric Carcinoma Progression

Zhang, X.Y.; Chan, Wai-Yee; Whitney, Bruce M.; Fan, Daiming; Chow, John; Liu, Y.; Ng, Enders K.; Chung, Sai Ho

doi:10.1006/clim.2001.5100

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…Robbins et al reported that tumor cell antigens in tumor tissue in situ or transferred to the blood circulation are likely to activate the body's immune system [23]. Zhang et al also reported that T cell clonal proliferation is detected in patients with rectal cancer; these patients had anti-tumor T lymphocytes [24]. Moreover, in some patients with tumors, monoclonal proliferative T lymphocytes activated by tumor antigen are transferred into PBMCs [25].…”

Section: Trav Cdr3 Familiesmentioning

confidence: 99%

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

Yang²,

Tang³

et al. 2012

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Purpose. To explore the expression of the TRAV gene in peripheral blood mononuclear cells (PBMCs) and in tumor-infiltrating lymphocytes (TILs) in the patients with breast cancer using a DNA melting curve (FQ-PCR) technique for T cell receptor (TCR) alpha chain CDR3 spectratyping. Peripheral blood samples and tissue samples were obtained from thirty breast cancer patients. Total RNA was extracted from PBMCs and tumor tissues and then reverse transcribed into cDNA. FQ-PCR was used to amplify the human TCR alpha chain CDR3 region with the primers to the TRAV and TRAC genes. TCR alpha chain CDR3 spectratyping and partial CDR3 sequencing were used to determine use of TRAV gene product in T cell responses. TCR alpha CDR3 spectratyping showed preferential usage of certain TRAV genes in the PBMCs and TILs of all patients with breast cancer. The frequencies of TRAV1.1, TRAV9, and TRAV29 exceeded 30% in PBMCs and the frequencies of TRAV1.1 and TRAV22 exceeded 30% in TILs. More than three quarters of the patients (23/30) overexpressed the same gene in both PBMCs and TILs; for example, patient-1 highly expressed TRAV9 in the PBMCs and TILs. Patients with positive or negative tumor markers of estrogen receptor (ER), progesterone receptor (PR), pS2, C-erbB-2, nm23, P53, and Ki-67 showed no significant common TRAV gene expression, but some TRAV gene preferential usage frequencies exceeded 20%. For example, five of eight patients positive for ER had high levels of expression of TRAV1.1 and TRAV32. Finally, the amino acid sequence of TCR CDR3 region showed some common motifs in some of the patients. Conclusions. TRAV gene expression was complex and diverse in the patients with breast cancer. The TRAV gene usage may be closely related to the diversity of breast tumor antigens and the differential immune responses observed in individual patients. Research into the immunological mechanism of T cells may provide guidance for individual T cell-directed therapy for breast cancer.

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Section: Trav Cdr3 Familiesmentioning

confidence: 99%

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

Yang²,

Tang³

et al. 2012

neo

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“…Differences in infiltrating TCR repertoires between normal and cancer tissues have been reported in several studies [14,[32][33][34]. In fact, immune responses to tumor antigens have been shown to be significant effect on disease progression in gastrointestinal cancers [35]. TCR of an individual can be accurately profiled using deep sequencing [36].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of profiling the peripheral blood T-cell receptor β-chain repertoire in gastric cancer

Liu¹,

Guo²,

Wu³

et al. 2020

Preprint

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Background: Gastric cancer is the third cause of cancer-related deaths worldwide, and is initially detected and attacked by the immune system through tumor-reactive T cells. The aim of this study was to determine the basic characteristics of the peripheral blood T-cell receptor (TCR) repertoire in patients with gastric cancer. Methods: High throughput sequencing was used to identify hyper-variable rearrangements of complementarity determining region 3 (CDR3) of the TCR β chain to comprehensively profile the TCR repertoire in peripheral blood samples from 6 advanced gastric cancer patients and 3 early gastric cancer controls. Results: The study showed that the TCR repertoire differed substantially between advanced cancer patients and early controls in terms of CDR3 clonotype, diversity and V/J segment usage. Specifically, low diversity reflected a worse immune status and prognosis in advanced gastric cancer. However the diversity of TCR was not significant difference in wild or mutation patients. Conclusion: TCR repertoire analysis served as a useful indicator of disease development and prognosis in gastric cancer and may be utilized to be biomarker for immunotherapy.

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“…According to previous studies [26][27][28], the subgroup of heteroideous T cells probably makes an important function in immune response to tumor cells, they can specifically function aiming to reciprocal antigen. In our study results, the characteristic of GMSP showed that the spectratyping of TRBV CDR3 exhibited a single-peak pattern in the course of anti-tumor, did the single peak represent the monoclonal T lymphocytes?…”

Section: Tablementioning

confidence: 99%

Primary exploration of CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma

Zhou

Luo

et al. 2010

Cancer Epidemiology

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T Cell Receptor Vβ Repertoire Expression Reflects Gastric Carcinoma Progression

Cited by 11 publications

References 18 publications

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

Characteristics of profiling the peripheral blood T-cell receptor β-chain repertoire in gastric cancer

Primary exploration of CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma

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T Cell Receptor Vβ Repertoire Expression Reflects Gastric Carcinoma Progression

Cited by 11 publications

References 18 publications

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

Characteristics of profiling the peripheral blood T-cell receptor β-chain repertoire in gastric cancer

Primary exploration of CDR3 spectratyping and molecular features of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma

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TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping

TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve (FQ-PCR) technique for TCR alpha chain CDR3 spectratyping