T-cell responses to allergens

Woodfolk, Judith A.

doi:10.1016/j.jaci.2006.11.008

Cited by 119 publications

(107 citation statements)

References 127 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…In the last decade, new concepts of specific immunotherapy have evolved based on recombinant expression of allergens. Hypoallergenic isoforms or mutants with reduced IgEbinding capacity would enable the use of a specific, safer and more efficient immunotherapy in the future (Woodfolk, 2007;Wagner et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…T-cells also contribute to induce tolerance to specific immunotherapy. During the vaccination process, a typical allergic Th2 response changes to a Thl, and, finally, a suppression of allergen-specific T-cell responses has been observed (Bohle, 2006(Bohle, , 2007Woodfolk, 2007). Evaluation of immunotherapies using peptides is generating great expectations.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Tordesillas¹,

Cuesta‐Herranz

González‐Muñoz

et al. 2009

Molecular Immunology

View full text Add to dashboard Cite

Keywords:Lipid transfer protein T-cell epitope Peptide immunotherapy Plant food allergy Peach Pru p3Lipid transfer proteins (LTPs), particularly peach Pru p 3, are the most relevant plant food allergens in the South of Europe, and, therefore, their allergic properties have been extensively studied. However, neither T-cell epitopes nor their effect on the patients' T-cell response has been investigated in any member of the LTP panallergen family. The objective of the present study was to map the major T-cell epitopes of Pru p 3, as well as to evaluate their induced T-cell response in peach-allergic versus control subjects. Thus, peripheral blood mononuclear cells (PBMCs) from 18 peach-allergic patients and Pru p 3-specific T-cell lines (TCLs) from 9 of them were cultured with Pru p 3 and with a panel of 17 derived peptides (10-mer overlapping in 5 amino acids representing the full sequence of Pru p 3). Proliferation in 5-day assays was carried out via tritiated-thymidine incorporation, while IL4 and IFN7 production was assessed via sandwich enzymelinked immunosorbent tests (ELISA) of TCL culture supernatants. The results were compared to those obtained from 10 non-peach allergic control volunteers. Two consecutive peptides showed the highest activation capacity. About 74% of PBMCs and TCLs recognized them, forming a single T-epitope: Pru p 365-80-Additionally, other specific T-cell epitopes were observed. Pru p 3 2 s-35 was detected by more than 60% of TCLs from peach-allergic patients, and Pru p 345-55 only activated PBMCs from control subjects. Interestingly, TCLs from patients were associated with a Th2-type, whereas control TCLs presented a Thltype cytokine response. The major immunogenic T-cell epitope identified in Pru p 3, Pru p 365-so, is a good candidate to develop new vaccines for hypersensitivity reactions associated with LTP allergens from Rosaceae fruits.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Tordesillas¹,

Cuesta‐Herranz

González‐Muñoz

et al. 2009

Molecular Immunology

View full text Add to dashboard Cite

show abstract

“…In an interesting study with intradermic injection of allergenic peptides without the ability to induce an IgE response, it was possible to observe lymphocyte trafficking from the vascular department (Haselden, 1999). Also, the intervention of T-reg cell in the mechanisms of immunomodulation of SIT are well established either by inducing the population of Th1 cells or reducing the number of Th2 (Woodfolk, 2007;Francis, 2003). Nevertheless, in these cells, it is not clear which is the specificity towards the allergen or what is the eligibility of the method of the induction (systemic or inhaled).…”

Section: Discussionmentioning

confidence: 99%

Specific Immunotherapy and Central Immune System

Pereira¹,

Loureiro²,

Tavares³

et al. 2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

View full text Add to dashboard Cite

“…However, although these approaches reduce the clinical symptoms, they are not curative. T-cells are key regulators of the pathogenesis of allergic disease 3 and it is well-established that allergen-specific immunotherapy (SIT) is the only way to modulate these immune reactions. When successful, therapeutic effects persist for many years without further treatment.…”

Section: Introductionmentioning

confidence: 99%

Seed-based oral vaccines as allergen-specific immunotherapies

Takaiwa

2011

Human Vaccines

View full text Add to dashboard Cite

T-cell responses to allergens

Cited by 119 publications

References 127 publications

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Specific Immunotherapy and Central Immune System

Seed-based oral vaccines as allergen-specific immunotherapies

Contact Info

Product

Resources

About