T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows

Oliveira, B.M.P.M.; Rasteiro, Ana M.; Correia, Alexandra; Pinto, Ana; Meireles, P.; Ferreira, Paula; Vilanova, Manuel; Teixeira, Luzia

doi:10.1038/s41598-019-39938-0

Cited by 7 publications

(13 citation statements)

References 38 publications

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“…Contrastingly, in bovines few studies characterized the cellular populations present in this tissue. In a previous work we showed diverse lymphoid subpopulations present in bovine adipose tissue, namely CD4 + and CD8 + CD3 + non-γδ T cells, γδ-T cells and NK cells, with the majority of T cells presenting an effector memory phenotype 20 . Here, we extended these observations by describing the complexity of myeloid cell populations that can be found in this tissue.…”

Section: Discussionmentioning

confidence: 89%

“…Despite this, cell populations in bovine adipose tissue remain poorly characterized. In a previous work we have shown the presence of CD8 + and CD4 + T cells, γδ T cells, as well as NK cells 20 . In this work, we aimed to characterize myeloid cell populations in bovine adipose tissue.…”

mentioning

confidence: 75%

“…Sample collection. Sample collection was done as previously described 20 . Briefly, samples of peripheral blood, mesenteric adipose tissue (MAT) and subcutaneous adipose tissue (SAT) were collected right after slaughter.…”

Section: Methodsmentioning

confidence: 99%

“…Peripheral blood leukocytes (PBL) were isolated by a previously described methodology 20 Antibodies. To construct a seven-colour panel, antibodies were first labelled with conjugation kits, accordingly to manufacturer instructions, as commercially available antibodies for bovines are conjugated to a very limited number of fluorochromes.…”

Section: Isolation Of Peripheral Blood Leukocytesmentioning

confidence: 99%

“…flow cytometry analysis. Flow cytometry analysis was done as previously described, with slight modifications 20 . Dead cells were excluded from our analysis by including in our panel a fixable viability dye (FVD).…”

Section: Isolation Of Peripheral Blood Leukocytesmentioning

confidence: 99%

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Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Oliveira

Pinto

Correia

et al. 2020

Sci Rep

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immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14 + CD11b + MHc-ii + CD45 + cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-A high CD11b −/+ CD14 − MHcii − CH138A − CD45 + cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138A + CD11b + phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A + CD11b − MHc-ii − phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.www.nature.com/scientificreports www.nature.com/scientificreports/ colour-flow cytometry revealed the presence of CD14 + cells, CD172a + cells, CD11c + cells and CD163 + cells in the stromal vascular fraction (SVF) of subcutaneous and omental adipose tissue 23,24 . Recently, Aylward et al. 25 reported the presence of CD172a + CD11b + cells, CD11c + MHC-II + cells, and CD11b + CD11c + cells in mesenteric adipose tissue. However, these markers are not exclusive to macrophages but common to other myeloid cell populations or can be even expressed in non-hematopoietic cells. As an example, studies in humans and mice have shown that CD14 can be expressed in non-myeloid cells 26 such as preadipocytes 27 and epithelial cells 28 . Also, CD172a is expressed by granulocytes such as eosinophils 29 and mast cells 30 beside its expression in monocytes/ macrophages. Since mast cells 31,32 and eosinophils 3,33 have already been described in adipose tissue, single markers analysis limits the interpretation of the data. Therefore, to characterize the different myeloid cell populations in bovine adipose tissue we used a multicolour flow cytometry panel associated with cell sorting and histochemistry/immunohistochemistry analysis. Our strategy allowed identification and isolation of macrophages, mast cells and neutrophils with macrophages being observed at different proportions in mesenteric adipose tissue (MAT) and subcutaneous adipose tissue (SAT). We also showed that CD45 negative cells represent more than 40% of all the cells in the adipose tissue SVF of MAT. A non-leukocyte populat...

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Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Isolation Of Peripheral Blood Leukocytesmentioning

confidence: 99%

Section: Isolation Of Peripheral Blood Leukocytesmentioning

confidence: 99%

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Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Oliveira

Pinto

Correia

et al. 2020

Sci Rep

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Review: Following the smoke signals: inflammatory signaling in metabolic homeostasis and homeorhesis in dairy cattle

Bradford

Swartz

2020

Animal

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Inflammatory cascades are a critical component of the immune response to infection or tissue damage, involving an array of signals, including water-soluble metabolites, lipid mediators and several classes of proteins. Early investigation of these signaling pathways focused largely on immune cells and acute disease models. However, more recent findings have highlighted critical roles of both immune cells and inflammatory mediators on tissue remodeling and metabolic homeostasis in healthy animals. In dairy cattle, inflammatory signals in various tissues and in circulation change rapidly and dramatically, starting just prior to and at the onset of lactation. Furthermore, several observations in healthy cows point to homeostatic control of inflammatory tone, which we define as a regulatory process to balance immune tolerance with activation to keep downstream effects under control. Recent evidence suggests that peripartum inflammatory changes influence whole-body nutrient flux of dairy cows over the course of days and months. Inflammatory mediators can suppress appetite, even at levels that do not induce acute responses (e.g. fever), thereby decreasing nutrient availability. On the other hand, inhibition of inflammatory signaling with non-steroidal anti-inflammatory drug (NSAID) treatment suppresses hepatic gluconeogenesis, leading to hypoglycemia in some cases. Over the long term, though, peripartum NSAID treatment substantially increases peak and whole-lactation milk synthesis by multiparous cows. Inflammatory regulation of nutrient flux may provide a homeorhetic mechanism to aid cows in adapting to rapid changes in metabolic demand at the onset of lactation, but excessive systemic inflammation has negative effects on metabolic homeostasis through inhibition of appetite and promotion of immune cell activity. Thus, in this review, we provide perspectives on the overlapping regulation of immune responses and metabolism by inflammatory mediators, which may provide a mechanistic underpinning for links between infectious and metabolic diseases in transition dairy cows. Moreover, we point to novel approaches to the management of this challenging phase of the production cycle.

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Cytokine production by bovine adipose tissue stromal vascular fraction cells upon Neospora caninum stimulation

Oliveira,

Sidónio,

Correia

et al. 2024

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In bovines few studies addressed the contribution of adipose tissue to the host immune response to infection. Here we evaluated the in vitro response of bovine adipose tissue stromal vascular fraction (SVF) cells to the protozoan parasite Neospora caninum, using live and freeze-killed tachyzoites. Live N. caninum induced the production of IL-6, IL-1β and IL-10 by SVF cells isolated from subcutaneous adipose tissue (SAT), while in mesenteric adipose tissue (MAT) SVF cell cultures only IL-1β and IL-10 production was increased, showing slight distinct responses between adipose tissue depots. Whereas a clear IL-8 increase was detected in peripheral blood leucocytes (PBL) culture supernatants in response to live N. caninum, no such increase was observed in SAT or MAT SVF cell cultures. Nevertheless, in response to LPS, increased IL-8 levels were detected in all cell cultures. IL-10 levels were always increased in response to stimulation (live, freeze-killed N. caninum and LPS). Overall, our results show that bovine adipose tissue SVF cells produce cytokines in response to N. caninum and can therefore be putative contributors to the host immune response against this parasite.

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T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows

Cited by 7 publications

References 38 publications

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Review: Following the smoke signals: inflammatory signaling in metabolic homeostasis and homeorhesis in dairy cattle

Cytokine production by bovine adipose tissue stromal vascular fraction cells upon Neospora caninum stimulation

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