T‐helper Cell Activity in Intestinal Lamina Propria*

Elson, Charles O.; Weiserbs, D.B.; Ealding, W; Machelski, E.

doi:10.1111/j.1749-6632.1983.tb26872.x

Cited by 13 publications

(5 citation statements)

References 11 publications

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“…In the experiments described here, B cells represented about 10% of the LP cells isolated; similar data have been reported by various authors (ARNAUD-BATTANDIER, 1982;LYSCOM & BRUETON, 1982;GOODACRE et al, 1979). Although IgA is normally the predominant isotype in LP cells (BEFUS et al, 1982) the helper T cell activity found in the LP population did not appear to be restricted to IgA synthesis, confirming the results of ELSON et al (1979) and of ELSON et al (1983) with LP cells of mouse.…”

Section: Discussionsupporting

confidence: 91%

Failure to demonstrate suppressor T cell activity in the lamina propria ofHymenolepis diminuta-infected rats

et al. 1993

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The aim of this study was to examine whether there was an increase of suppressor T cells, relative to helper T cells, in the intestinal lamina propria of Hymenolepis diminuta-infected rats, a condition which might allow the parasite to survive for the life-span of the host. Lamina propria cells were isolated by enzymatic procedure. All lymphocytes were passed over nylon wool columns in order to remove B cells; then the T cells were purified by a panning technique using monoclonal anti-T cell antibodies. Changes in the mitogen-stimulated synthesis of 1gM, IgG and IgA by normal peripheral blood indicator lymphocytes, as measured by sandwich ELISA, was used as an index of help and/or suppression. No significant suppressor T cell activity was observed in the cultures, either from control or infected intestine.

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Section: Discussionsupporting

confidence: 91%

Failure to demonstrate suppressor T cell activity in the lamina propria ofHymenolepis diminuta-infected rats

et al. 1993

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“…The sensitivity of this assay compared with other available techniques for detection of antigen-specific gut mucosal responses appears to be very high. The immunohistochemical detection of antibody-containing cells (ACC) in tissue sections or the measurement of specific antibodies in intestinal washings or in supernatants from cell cultures can only poorly detect antigenspecific responses after a single oral priming immunization with CT, whereas the method presented in this paper registered substantial primary antitoxin SFC responses in the lamina propria, especially when the sensitivity-enhancing vaseline border ring method was used [8,12,13,17]. Furthermore, the lower limit of sensitivity ofthe ACC technique has been reported to be around 100 ACC/mm\ with peak responses of 10,000-20.000 ACC/mm' to oral immunizations with CT [17].…”

Section: Discussionmentioning

confidence: 99%

“…Despite this progress, the study of antigenspecific responses of the local immune system and the cellular mechanisms responsible for mounting such a response has been hampered by the lack of suitable methods for assessing immunoglobulin secretion at the single cell level. To date, most studies on specific antibody responses by the gut mucosal immune system have been performed with immunohistological methods demonstrating antibody-containing cells (ACC) in tissue sections, or ELISA methods determining specific antibodies in intestinal washings or supernatants from in vitro cultured gut lymphocytes [8,12,13,17]. These methods have limitations In that they do not provide any possibility of studying regulatory influences on individual B lymphocytes of the gut.…”

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confidence: 99%

A Sensitive Method for the Detection of Specific Antibody Production in Different Isotypes from Single Lamina Propria Plasma Cells

Lycke

1986

Scand J Immunol

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A sensitive and reproducible method for the detection of specific antibody production (or total immunoglobulin secretion) at the single cell level from isolated lamina propria lymphocytes was developed. The cells were prepared from mouse intestinal mucosa by enzyme extraction with collagenase, and antibody secretion was demonstrated with a solid phase enzyme-linked immunospot (ELISPOT) assay. Oral immunizations with cholera toxin or keyhole limpet haemocyanin to mice gave high numbers of highly antigen-specific spot-forming cells (SFC) among isolated lamina propria lymphocytes. Spots were shown to result from active synthesis of immunoglobulin in vitro. The variation in SFC numbers between individual animals after a given protocol of oral immunizations was found to be 25% and between equal groups analysed on different occasions, 12%. Kinetics of primary as well as secondary immune responses after oral immunizations with cholera toxin were easily monitored. A single dose of cholera toxin gave rise to 230 antitoxin SFC/10(7) isolated lamina propria lymphocytes. Each additional dose stimulated to increasing numbers of specific SFC with roughly 7000 antitoxin SFC/10(7) cells after five immunizations. Monitoring of day-by-day responses after oral booster immunizations demonstrated peak SFC numbers on day 8 after antigen administration. The total number of immunoglobulin-secreting (Ig) cells and the isotype distribution of specific SFC could also be determined. In the peak antitoxin response, 8% of the isolated total Ig-secreting lamina propria cells were active against cholera toxin, and of these 80% were producing IgA. This method has also been successfully used in humans and rabbits to demonstrate specific antibody production by single lamina propria plasma cells.

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“…Several observations in this study support this conclusion. MacDermott et ai (1981), Clancy et al (1984) and Elson et al (1983) provide comparative data on mucosal lymphocytes. Firstly, the pattern of immunoglobulin isotype secretion was found to be similar to that observed in mucosal lymphocyte cultures.…”

Section: Discussionmentioning

confidence: 99%

“…1977;Goodacre et al, 1979;MacDermott et al. 1981;Clancy et a/.. 1984;Elson et a!., 1983^. Co-culture of polyclonally activated T and B cells has been used to examine the regulatory effect of T cells on immunoglobulin secretion (Elson. Heck andStrober.…”

Section: Introductionmentioning

confidence: 99%

STUDY OF LYMPHOCTYE INTERACTION IN HUMAN MESENTERIC LYMPH NODES IN VITRO

Clancy

Chipchase

et al. 1984

Aust J Exp Biol Med

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Summary The immune function of lymphocytes isolated from human mesenteric lymph nodes (MLN) has been examined. Large yields of viable lymphocytes were obtained from MLN without the need for using enzyme or mucolytic agents. In comparison to gut mucosal lymphocytes MLN lymphocytes (MLNL) were technically easier to manipulate and to use in providing a more quantitative analysis of T‐B cell interaction. The results presented indicate that the pattern of immunoglobulin secretion and immunoregulution of lymphocytes isolated from MLN was similar to that in cells isolated from the lamina propria, supporting that MLN constitute an important component of gut‐associated lymphoid tissue (GALT).

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T‐helper Cell Activity in Intestinal Lamina Propria*

Cited by 13 publications

References 11 publications

Failure to demonstrate suppressor T cell activity in the lamina propria ofHymenolepis diminuta-infected rats

Failure to demonstrate suppressor T cell activity in the lamina propria ofHymenolepis diminuta-infected rats

A Sensitive Method for the Detection of Specific Antibody Production in Different Isotypes from Single Lamina Propria Plasma Cells

STUDY OF LYMPHOCTYE INTERACTION IN HUMAN MESENTERIC LYMPH NODES IN VITRO

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