T Lymphocyte Adhesion to Human Synovial Fibroblasts. Role of Cytokines and the Interaction Between Intercellular Adhesion Molecule 1 and CD11a/CD18

Krzesicki, Raymond F.; Fleming, William E.; Winterrowd, Greg E.; Hatfield, Cheryl A.; Sanders, Martin; Chin, Jia En

doi:10.1002/art.1780341007

Cited by 40 publications

(24 citation statements)

References 53 publications

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“…In vitro studies revealed that human T cells have the ability to adhere to synoviocytes as well as to dermal fibroblasts via CD2/CD58 (LFA-3) and LFA-1 (CD11a/CD18)/CD54 (ICAM-1) interactions (12,13,49). Furthermore, the cellular adhesion between T cells and synoviocytes induces the production of IL-1␤ by synovial cells in part through LFA-1-ICAM-1 interaction (17).…”

Section: Discussionmentioning

confidence: 99%

Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines

Rezzonico¹,

Burger²,

Dayer³

1998

Journal of Biological Chemistry

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In many inflammatory diseases where tissue remodeling occurs, T cells are in close contact with mesenchymal cells. We investigated the effect of direct cell-cell contact between peripheral blood T lymphocytes or HUT-78 lymphoma cells and dermal fibroblasts or synoviocytes on the deposition of the major extracellular matrix components: types I and III collagen. Incubation of dermal fibroblasts and synoviocytes with plasma membrane preparations from resting T cells slightly increased the production of collagen I but did not significantly affect that of collagen III. Conversely, direct contact with either plasma membranes or fixed phytohemagglutinin/phorbol myristate acetate-activated T cells markedly inhibited the synthesis of types I and III collagen by 60-70% in untreated dermal fibroblasts and synoviocytes and by 85% in transforming growth factor ␤-stimulated fibroblasts. This decrease of collagen synthesis was abrogated when fixed T cells were separated physically from fibroblasts, demonstrating that direct contact between the two cell types was necessary. This inhibition was associated with a marked decrease in steady-state levels of pro-␣1(I) and pro-␣1(III) collagen mRNAs. T cell contact decreased the transcription rate but did not significantly alter the stability of the ␣1(I) and ␣1(III) transcripts. Finally, using neutralizing antibodies or cytokine inhibitors we provide evidence that this inhibition of extracellular matrix production mediated by T cell contact was partially due to additive effects of T cell membrane-associated interferon ␥, tumor necrosis factor ␣, and interleukin-1␣.

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Section: Discussionmentioning

confidence: 99%

Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines

Rezzonico¹,

Burger²,

Dayer³

1998

Journal of Biological Chemistry

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“…The integrin CD11a/CD18 (LFA-1) clusters at the contact point between T cell and FLS (8) and T cell adherence to FLS is inhibited by blocking Ab to LFA-1 (9). FLS also express significant MHC class II in vivo (10), which can be reinduced in vitro (11,12).…”

Section: Interactions Of T Cells With Fibroblast-like Synoviocytesmentioning

confidence: 99%

Interactions of T Cells with Fibroblast-Like Synoviocytes: Role of the B7 Family Costimulatory Ligand B7-H3

Tran

Thacker

Louie

et al. 2008

The Journal of Immunology

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Fibroblast-like synoviocytes (FLS) and T cells can activate each other in vitro, and in vivo interactions between these cells may be important in rheumatoid arthritis (RA), yet FLS lack significant expression of CD28 ligands. We sought to identify molecules homologous to CD28 ligands that are strongly expressed by FLS, and documented strong B7-H3 expression on FLS and by fibroblasts of other tissues, which was unaffected by a variety of cytokines. Western blot analysis of FLS lysates showed predominant expression of the larger, four Ig-like domain isoform of B7-H3. Immunohistological sections of RA synovial tissue showed strong staining for B7-H3 on FLS. Cells expressing B7-H3 were distinct from but in close proximity to cells that expressed CD45, CD20, and CD3. Confocal microscopy of FLS and T cell cocultures showed localization of B7-H3 in the region of the T cell-FLS contact point, but distinct from the localization of T cell CD11a/CD18 (LFA-1) and FLS CD54 (ICAM-1). Reduction of B7-H3 expression on FLS by RNA interference affected interactions of FLS with resting T cells or cytokine-activated T cells. Resting T cells showed increased production of TNF-α, IFN-γ, and IL-2, whereas cytokine-activated T cells showed reduced cytokine production relative to control. However, cytokine production by T cells activated through their TCR was not notably altered by knock down of B7-H3. These observations suggest that B7-H3 may be important for the interactions between FLS and T cells in RA, as well as other diseases, and the outcome of such interactions depends on the activation state of the T cell.

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“…Although other authors reported the induction of periapical lesion by allowing pulp exposure of rat molars 7,8) , studies on laboratory animals using m-CT over time are very few 5) . In conventional R_mCT, the x-ray tube and sensor are fixed therefore the image is difficult to aim since the tail and head would contact the tube while rotating in vivo, which is essential in sample preparation.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Experimental Periapical Inflammatory Lesions in Mice

Osuga

Matsuda

Shoumura

et al. 2013

J. Hard Tissue Biology.

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Although studies on the formation of apical periodontitis have somehow been carried out but detailed cellular dynamics remain unclear. We recently established an experiment that could easily be performed using ddY mice. First, under general anesthesia using isoflurane inhalation, the coronal portion of the maxillary first molar was penetrated using a round bur and drill with water irrigation causing pulp exposure until the root apex. Micro computed tomography (R_mCT) was taken over time during observation. Four weeks later, R_mCT confirmed the presence of a radiolucent image at the apex of the tooth, which was then removed for histological examination. The results showed that granulation tissue with fibrosis had gradually formed at the periphery of the abscess. The present method confirmed the effectiveness of the experimental mode to exmine the formation of chronic inflammatory lesions at the root apex.

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T Lymphocyte Adhesion to Human Synovial Fibroblasts. Role of Cytokines and the Interaction Between Intercellular Adhesion Molecule 1 and CD11a/CD18

Cited by 40 publications

References 53 publications

Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines

Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines

Interactions of T Cells with Fibroblast-Like Synoviocytes: Role of the B7 Family Costimulatory Ligand B7-H3

Establishment of Experimental Periapical Inflammatory Lesions in Mice

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