T lymphocyte subpopulations and B lymphocyte cellsin caecum and spleen of chicks infected with Salmonella enteritidis

Asheg, Abdulatif; Levkut, M.; Révajová, Viera; Ševčíková, Zuzana; Kolodzieyski, L.; Pistl, J.

doi:10.1078/0065-1281-00666

Cited by 12 publications

(12 citation statements)

References 9 publications

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“…In addition here young chickens were investigated where the intestinal immune system is immature, which could be a reason that no increase in CD4 + T-cells was found. For CD8 + cells, we found an increase after the Salmonella infection in both chicken lines, but faster in time post infection in line F. An increase in the amount of CD8 + cells was also found in the caeca after a Salmonella infection [1,6], but also decreases in the amount of CD8+ cells in the caeca are reported [18]. In the oviduct of laying hens, the numbers of CD8 + cells were increased after a Salmonella infection [4,25,26].…”

Section: Discussionmentioning

confidence: 52%

“…The number of CD4 + was decreased in line F in response to the Salmonella infection, but not in line S. This is surprising because in most studies an increase in the amount of CD4 + T-cells in the caeca was found after a Salmonella infection [1,6]. Also in mice the number of CD4 + T-cells increased in the gut following Salmonella challenge [2,15].…”

Section: Discussionmentioning

confidence: 89%

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Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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-Besides infection in humans, Salmonella enteritidis can also cause serious illness in young chickens. However, the genetic and immunological parameters important for the disease in chickens are not well characterized. In this study, processes in the chicken intestine in response to a Salmonella infection were investigated in two different chicken lines. One-day-old chickens were orally infected with Salmonella. T-cell subpopulations, phagocytic properties of intestinal mononuclear cells and RNA expression levels of the jejunum were investigated. The two chicken lines differed in the amount of cfu in the liver and growth retardation after the infection. Differences in phagocytic activity of intestinal mononuclear cells were found between control and Salmonella infected chickens. The number of CD4 + T-cells of the intestine decreased after the Salmonella infection in one chicken line, while the number of CD8 + T-cells increased in both chicken lines, but the time post infection of this increase differed between the lines. In one chicken line the expression levels of the genes carboxypeptidase M and similar to ORF2 decreased after the Salmonella infection, which might be related to a decrease in the amount of macrophages. With the microarray, ten genes were found that were regulated in only one of the chicken lines, while we found six genes regulated in response to the infection in both chicken lines. So differences in genetic background of the chickens influence the intestinal host response of the Salmonella infection as observed by phagocytic activity, gene expression and changes in the number of T-cell subpopulations and macrophages.immunology / microarray / Salmonella / T-cell

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 89%

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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“…Segundo Arstila et al (1994), as células T CD4+ estão envolvidas com a ativação de macró-fagos e a modulação da resposta imune, enquanto que célu-las T CD8+ desempenham funções relacionadas com a eliminação do antígeno (Zou et al 2006). Asheg et al (2002) Agradecimentos.-À empresa Novartis Saúde Animal Ltda pela disponibilização do produto e auxílio durante a pesquisa.…”

Section: Resultsunclassified

Uso de probiótico sobre a ativação de células T e controle de Salmonella Minnesota em frangos de corte

et al. 2013

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To evaluate the effect of probiotics on the immune response of broiler chickens challenged with Salmonella Minnesota (SM), 60 chickens were divided into three groups: CN -Birds that were not inoculated with SM (negative control), CP -birds inoculated with SM (positive control) and Probiotic-birds supplemented with probiotic consisting of Lactobacillus acidophilus, L. plantarium, L. rhamnosus, L. bulgaricus, Enterococcus faecium, Streptococcus thermophilus and Bifidobacterium bifidum in diet and inoculated with SM. At 14 days the birds were challenged with SM, and at 7 and 35 days were quantified goblet cells, CD4 + and CD8 + intestinal mucosa of the ileum and cecum. Birds supplemented with probiotics, at 7 days of age showed a significant increase (P≤0.05) of goblet cells in the ileum and CD4 + and CD8 + cells in the cecum. At 35 days there were significant (P≤0.05) of CD8 + cells in birds inoculated on CN and Probiotic. The use of probiotics provided a significant reduction (P≤0.05) of Salmonella sp. counts.

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“…No presente estudo foi observado que aves desafiadas com SE e ST apresentaram maior contagem de células CD4+ no íleo e ceco das aves aos 14 e 20 dias, quando comparado aos demais sorovares e o controle negativo, o que também foi observado em outros estudos onde foi evidenciado maior expressão destas células após infecção oral com SE (Sasai et al 2000, Asheg et al 2002 e com ST (Berndt & Methner 2001, Beal et al 2004). Em contrapartida, Hemert et al (2007) mostraram diminuição na contagem destas células no jejuno ao primeiro e ao quinto dia pós-inoculação oral de 0,2 ml de 10 5 UFC/ml de Salmonella Enteritidis quando comparado com o controle negativo.…”

Section: Discussionunclassified

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

et al. 2015

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RESUMO.-Objetivou-se com o presente estudo comparar o efeito de diferentes sorovares de Salmonella na resposta imune local da mucosa do intestino de frangos de corte. Aos sete dias de idade, as aves foram desafiadas com os sorovares S. Enteritidis, S. Typhimurium, S. Senftenberg, S. Mbandaka e S. Minnesota. Foi observado que todos os sorovares testados foram capazes de colonizar o intestino das aves sendo possível o isolamento de Salmonella em suabes de cloaca, 48 h após inoculação. De maneira geral, as aves do grupo controle negativo, que não foram desafiados apresentaram quantidade significativamente menor de células imunológicas na mucosa intestinal do que as aves desafiadas. Porém, verificou--se que os sorovares de Salmonella, utilizados neste estudo, apresentaram diferentes efeitos sobre a dinâmica celular da mucosa do íleo e ceco e afetaram de modo diferente o ganho de peso e ganho médio diário das aves demonstrando distintos graus de patogenicidade. Os sorovares Enteritidis e Typhimurium apresentaram um efeito mais intenso tanto no desempenho quanto na mobilização de células imunoló-gicas na mucosa intestinal de frangos de corte. The study was designed to compare the effect of different Salmonella serovars in immune response across the count of CD8+ cells, CD4+ cells, goblet cells and macrophages in the gut mucosa of broilers. During the experimental inoculation at 7 day-old were used Salmonella enterica subspecies enterica sorovars Enteritidis, Typhimurium, Senftenberg, Mbandaka and Minnesota. It was observed that all serovars tested were capable of contaminating the poultry being possible counts of Salmonella in cloacal swabs, 48 h after inoculation and into the crop and cecum, at 14 and 20 day-old. Serovars tested had different effects on broiler performance assessed at 20 days. In the mucosa of the ileum and cecum of broilers, it was observed that some of the serotypes increased CD8 + cells, CD4 + cells, goblet cells and macrophages compared to the negative control group both at 14 and at 20 day-old. S. Enteritidis and S. Typhimurium are the serovars that showed the more intense effect in live performance and in the immune system of birds showing pathogenic characteristic; generally the broilers of the negative control showed significantly less immune cells on the intestinal mucosa than broilers inoculated experimentally. However, it was found that the Salmonella serovars used in this study had different effects on the cellular dynamics of the mucosa of the ileum and cecum and differently affect weight gain and average daily gain of poultry showing different levels of pathogenicity. Avaliação da resposta imunológica da mucosa intestinal

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T lymphocyte subpopulations and B lymphocyte cellsin caecum and spleen of chicks infected with Salmonella enteritidis

Cited by 12 publications

References 9 publications

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Uso de probiótico sobre a ativação de células T e controle de Salmonella Minnesota em frangos de corte

Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella

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