Tabrizicola aquatica gen. nov. sp. nov., a novel alphaproteobacterium isolated from Qurugöl Lake nearby Tabriz city, Iran

Abstract

A novel Gram-negative, aerobic, non-motile and rod-shaped bacterium was isolated from Qurugöl Lake near Tabriz city. The bacterium grew chemoorganolheterotrophically and chemolithoautotrophically. However, photo-organoheterotrophic, photo-lithoautotrophic and fermentative growth could not be demonstrated. The presence of photosynthesis genes pufL and pufM was not shown and photosynthesis pigments were not formed. Strain RCRI19(T) grew without NaCl and tolerated up to 3 % NaCl. Growth occurred at pH 6-9 (optimum, pH 7) and 15-55 °C (optimum 40-45 °C). Vitamins were not required for growth. The major fatty acids are C18:1 ω7C, 11-methyl C18:1 ω7C, C18:0 3-OH. The predominant respiratory quinone is ubiquinone Q-10. The G+C content of genomic DNA is 65.9 mol%. Analysis of 16S rRNA sequences showed that strain RCRI19(T) has the highest similarities with uncultured environmental sequences followed by members of the genera Rhodobacter (≤95.75 %), Haematobacter (≤95.53 %), Gemmobacter (≤95.17 %) and Falsirhodobacter (94.60 %) in the family Rhodobacteraceae. DNA-DNA relatedness between strain RCRI19(T) and the closest phylogenetically related strain, Rhodobacter blasticus LMG 4305(T), was 20 %. Based on its phenotypic and chemotaxonomic characteristics and considering that it does not form photosynthetic pigments and is unable to grow phototrophically, it is concluded that strain RCRI19(T) cannot be included into the genus Rhodobacter and any of the other related genera. Therefore, we propose to place the new bacterium into a new genus and species for which the name Tabrizicola aquatica gen. nov. and sp. nov. is proposed. The type strain is RCRI19(T) (=BCCM/LMG 25773(T )= JCM 17277(T )= KCTC 23724(T)).

Antibacterial and Antifungal Activity of Novel Freshwater Bacterium Tabrizicola aquatica as a Prominent Natural Antibiotic Available in Qurugol Lake

“…19 Here, the antimicrobial production ability of Tabrizicola aquatic RCRI19 T , a new bacterium isolated from Qurugol Lake located neighboring Tabriz city, Iran, is reported for the first time. 13 The whole genome of Tabrizica aquatica was sequenced. It is available in the National Center for Biotechnology Information (NCBI) with accession number of NZ_PJON00000000.1.…”

“…The bacterium is a gramnegative, non-motile and rod-shaped and can grow chemoorganohetrotrophically and chemo-lithoautotrophically. 13 The present study evaluates the antimicrobial activity of Tabrizicola aquatica RCRI19 T which was isolated from Qurugol Lake neighboring Tabriz city, Iran.…”

Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis

“…In this study, we especially focused on the gut micro-biota of freshwater fishes because, unlike other wildlife, little is known about the gut microbiota of freshwater organisms. As part of an investigation into the microbial diversity of various Korean indigenous freshwater fish, a Tabrizicola-like strain was isolated from the gut of oily bitterling (Acheilognathus koreensis) Tarhriz et al proposed the genus Tabrizicola in the family Rhodobacteraceae [8]. At the time of writing, the genus contains four species, Tabrizicola aquatica RCRI19 T , as the type species, Tabrizicola sediminis DRYC-M-16 T , Tabrizicola alkalilacus DJC T and Tabrizicola fusiformis SY72 T .…”

Halomonas lactosivorans sp. nov., isolated from salt-lake sediment

“…Colony morphology and colour were determined on cells grown on MA medium [41] after incubation at 28 °C for 3 days. Cell morphology of the strain CFH 90008 T was observed by using light microscopy (model BH2, Olympus), scanning electron microscopy (ESEM-TMP) and transmission electron microscopy (JEM 2100).…”