Tachykinin receptor types: Classification and membrane signalling mechanisms

Guard, Steven; Watson, Steve P.

doi:10.1016/0197-0186(91)90180-l

Cited by 344 publications

(130 citation statements)

References 105 publications

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“…into nuclei of Xenopus laevis oocytes. Neurokinins increased current amplitude (data not shown) with the relative rank-order of potency: NKB>NKA>SP which is consistent with the actions of an NK-3 receptor [2,11,12,28]. Half-maximally effective concentrations of NKB and NKA were 4 nM and 40 nM, respectively.…”

Section: Resultssupporting

confidence: 71%

“…Neurokinins are a family of neuropeptides displaying wide-spread distribution and diverse biological activities including regulation of neurotransmission, inflammation and perception of pain [1][2][3]. Substance P ($P), neurokinin A (NKA) and neurokinin B (NKB) are mammalian neurokinins showing limited selectivity towards, respectively, NK-1, NK-2 and NK-3 receptors.…”

Section: Introductionmentioning

confidence: 99%

“…Pharmacological and biochemical studies, as well as molecular cloning, have shown that all three neurokinin receptors are members of the G-proteincoupled receptor super-family possessing seven putative transmembrane regions [4]. NK-3 receptors have been identified in membranes from guinea pig myenteric plexus and cerebral cortex [5] as well as brain slices of rat, mouse and guinea pig [2,3,6,7]. NK-3 receptors also mediate increased phosphoinositide hydrolysis in guinea pig ileum and neonatal rat spinal cord [2,8].…”

Section: Introductionmentioning

confidence: 99%

“…NK-3 receptors have been identified in membranes from guinea pig myenteric plexus and cerebral cortex [5] as well as brain slices of rat, mouse and guinea pig [2,3,6,7]. NK-3 receptors also mediate increased phosphoinositide hydrolysis in guinea pig ileum and neonatal rat spinal cord [2,8]. In addition, NK-3 receptors depolarize guinea pig myenteric neurons to augment release of acetyleholine [9][10][11], stimulate contraction of the rat portal vein [12] and modulate both serotinergieand ¢holinergic-dependent behavioural responses in mouse and rat [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterisation, expression and localisation of human neurokinin‐3 receptor

Buell¹,

Schulz²,

Arkinstall³

et al. 1992

FEBS Letters

101

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The complete amino acid sequence of the human neurokinin-3 receptor was deduced by DNA sgqumtco analysis of human genomic fragments. Comparison of the predicted primary structure with those for the human neurokinin receptors 1 and 2 shows a highly conserved pattern of seven hydrophobi¢ regions with maximum divergence oceuring at the amino-and carboxy-terrnini, The position of intron-exon junctions are identical to those in other reported neurokinin genes, Using a chimeric genomic.cDNA gone, the human NK-3 receptor was expressed in Xenopus laevts oooytes where it mediates membrane conductance changes in response to its agonist, neurokinin B. More significantly, expression of the 8ene in mammalian cells resulted in detection of receptor binding as well as neurokinin-stimulated calcium mobilization and araehidonie acid release, all displaying the pharmacological characteristics expected of a nearokinin-3 receptor, By using the polymerase chain reaction we have shown that mRNA for the human neurokinin-3 receptor is expressed predominantly in the egntral nervous system,

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular characterisation, expression and localisation of human neurokinin‐3 receptor

Buell¹,

Schulz²,

Arkinstall³

et al. 1992

FEBS Letters

101

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“…Application of tachykinins to the intestine induces various effects including excitation of muscle, increase in blood flow and secretion (Holzer and Holzer-Petsche, 1997). The diverse effects of these peptides are mediated through three receptors called NK1, NK2, and NK3 (Guard and Watson, 1991;Grady et al, 1996).…”

mentioning

confidence: 99%

Interactions of Serotoninergic, Cholinergic, and Tachykinin‐Containing Nerve Elements in the Rabbit Small Intestine

Denes

Wilhelm

NÉMeth

et al. 2009

The Anatomical Record

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This report presents novel results on the effects of serotonin (5-HT) on longitudinal muscle contractions in the rabbit ileum and the interactions of serotonin with some neuronal elements of the myenteric plexus. We showed previously that serotonin-triggered contractions involved two mechanisms in the rabbit ileum: neuronal excitation (via 5-HT 2 receptors in the neurons) and direct muscular stimulation (via 5-HT 4 receptors in the muscle). Here, we focus on the neuronal 5-HT 2 receptor pathway and report further pharmacological and immunocytochemical data clarifying the details of the mechanisms. We observed that antagonists for neurokinin (NK1 and NK2) receptors partially blocked the serotonin response, but NK3 receptor antagonists had no effect. Pretreatment by atropine (ATR) eliminated the NK1 receptor antagonist resistant contractions. In contrast, the NK1 antagonist did not depress the ATR-resistant contraction when ATR was added first. 5-HT 2 receptor agonist-induced contractions were partially suppressed by ATR, hexamethonium, and NK1 or NK2 receptor antagonists. In conclusion, serotonin acting through 5-HT 2 receptors could stimulate interneurons and excitatory motor neurons. Immunocytochemical staining revealed an extensive tachykinin-immunoreactive (IR) network in the myenteric plexus. Approximately 52% of all myenteric neurons were labeled. 5-HT-IR fibers could be detected around both choline acetyltransferase-and tachykinin-IR cells, suggesting functional relationships between them. Consistent with our pharmacological observations, we found that immunopositive nerve elements for 5-HT 2A receptor and double-labeled immunostaining revealed a remarkable overlap between tachykinin-IR neurons and 5-HT 2A -IR elements. Anat Rec, 292:1548Rec, 292: -1558Rec, 292: , 2009. Key words: ileum; 5-HT; tachykinins; 5-HT 2 receptors; rabbit One of the principal regulators of digestion in mammals is the enteric nervous system. Enteric neurons coordinate a variety of functions, such as the secretion of digestive enzymes, control of absorption, and the regulation of motility. Intestinal smooth muscle contractility is organized by polarized reflex pathways through both excitatory and inhibitory neurons (Goyal and Hirano, 1996;Kunze and Furness, 1999;Olsson and Holmgren,

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Localisation of NK1 receptor immunoreactivity to neurons and interstitial cells of the guinea-pig gastrointestinal tract

et al. 1996

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Tachykinins, including substance P, neurokinin A, and neuropeptides K and gama, are expressed widely in the peripheral nervous system where they affect smooth muscle contraction, exocrine gland secretion, vascular permeability, and neurotransmission. Substance P, the preferred ligand for the NK1 receptor, is found in high concentrations in the enteric nervous system. In the present study, the localisation and distribution of the NK1 receptor was studied throughout the gastrointestinal tract of the guinea-pig by using a polyclonal antiserum raised against the C-terminal 15 amino acids of the NK1 receptor. Co-localisation with other neuronal markers was examined in the ileum. Nerve cell bodies reactive for the NK1 receptor were found in the myenteric plexus of all regions and the submucous plexus of the small and large intestines. In the small intestine, the interstitial cells of Cajal were also immunoreactive. Immunoreactivity was largely confined to cell surfaces. Almost all immunoreactive myenteric nerve cells had Dogiel type I morphology, and most of these were immunoreactive for nitric oxide synthase, a transmitter of inhibitory neurons to the muscle and of descending interneurons. Neuropeptide Y-containing secretomotor neurons in the submucous and myenteric plexuses also exhibited NK1 receptor immunoreactivity. NK1 receptors were present on a minority of tachykinin immunoreactive neurons of submucous ganglia. The results suggest that receptors on the longitudinal muscle might not be conventional NK1 receptors, that excitation of the circular muscle of the ileum is indirect, perhaps via the interstitial cells of Cajal, and that enteric inhibitory neurons may be excited via NK1 receptors.

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Tachykinin receptor types: Classification and membrane signalling mechanisms

Cited by 344 publications

References 105 publications

Molecular characterisation, expression and localisation of human neurokinin‐3 receptor

Molecular characterisation, expression and localisation of human neurokinin‐3 receptor

Interactions of Serotoninergic, Cholinergic, and Tachykinin‐Containing Nerve Elements in the Rabbit Small Intestine

Localisation of NK1 receptor immunoreactivity to neurons and interstitial cells of the guinea-pig gastrointestinal tract

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