Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry

Abstract

Nano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) approach was validated based on the technical specification (TS) 19590:2017 of the international organization for standardization (ISO). The TS has been adapted to the hyphenated setup. The quality criteria (QC), e.g., linearity of the calibration, transport efficiency, were investigated. Furthermore, a cross calibration of the particle size was performed with values from dynamic light scattering (DLS) and transmission electron microscopy (TEM). Due to an additional Y-piece, an online-calibration routine was implemented. This approach allows the calibration of the ICP-MS during the dead time of the chromatography run, to reduce the required time and enhance the robustness of the results. The optimized method was tested with different gold nanoparticle (Au-NP) mixtures to investigate the characterization properties of HDC separations for samples with increasing complexity. Additionally, the technique was successfully applied to simultaneously determine both the hydrodynamic radius and the Au-NP content in liposomes. With the established hyphenated setup, it was possible to distinguish between different subpopulations with various NP loads and different hydrodynamic diameters inside the liposome carriers.

Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles

“…103 Rh was used as internal standard. The recovery of this method was within the range of 86% to 120% [61]. The limit of detection (LOD) and limit of quantification (LOQ) were calculated as 3 and 10 times the standard deviation of the blank samples, respectively.…”

The Golden Liposomes: Preparation and Biomedical Applications of Gold-Liposome Nanocomposites

“…For instance, morphological information obtained from SEM and TEM could be further correlated with the optical absorption spectra of the nanocomposite where coloration of liposomes and AuNP and its unique plasmonic optical extinction in the Visible-NIR region could confirm the formation of the AuNP-liposome nanocomposites and its size that correlates with unique wavelengths [122,130,169]. Moreover, correlating the data obtained by FTIR, XPS, and ICP-MS could provide insights on the overall structure, composition, and surface properties of the AuNP-liposome nanocomposites [133,170]. take compared to CTAB-mediated AuNR.…”

Analytical applications of single particle inductively coupled plasma mass spectrometry: a comprehensive and critical review

“…Under less complex conditions, HDC-SP-ICP-MS has been successfully applied to simultaneously determine both the hydrodynamic radius and the content of Au nanoparticles in liposomes used as carriers of Au nanoparticles. 289 It was possible to distinguish between subpopulations of liposomes with different hydrodynamic diameters and various nanoparticle loads. The application of HDC-SP-ICP-MS to the analysis of river and wastewaters under optimized conditions did not allow to detect silver nanoparticles in the samples, although Cucontaining nanoparticles could be identified.…”