TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation

Hidalgo, Gabriela dos Santos; Meola, Juliana; Silva, Júlio César Rosa e; Paz, Cláudia Cristina Paro de; Ferriani, Rui Alberto

doi:10.1016/j.fertnstert.2011.06.052

Cited by 28 publications

(28 citation statements)

References 31 publications

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“…By comparing their peptide mass fingerprints to the IPI human database, eleven known proteins were identified and found to be a series of proteins related to proliferation, differentiation, apoptosis and cell survival (34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47). The SSX proteins are thought to act as transcriptional repressors through transcription repression domain (48).…”

Section: Discussionmentioning

confidence: 99%

“…TAGLN (transgelin) is believed to be related to cell differentiation, cell migration, and the formation of podosomes and involved in tissue invasion and matrix remodeling (34,35,39). It has been confirmed that TAGLN is able to promote migration and invasion of CSCs (42). Microtubules are cytoskeleton that play critical roles in cell division.…”

Section: Discussionmentioning

confidence: 99%

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Cancer/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ERα signaling

et al. 2012

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Abstract. Cancer/testis antigen (CTA) SSX2, which is silent in most normal adult tissues and expressed in various malignant tumors, has been identified for decades. Expression of SSX in tumors has been associated with advanced stages and worse patient prognosis. However, little is known about its role in breast cancer. The SSX2 expression plasmid constructed was stably transfected into the breast cancer cell line MCF-7. The influence of SSX2 on MCF-7 cells was assessed using MTT assay, flow cytometry, transwell invasion assay and in vivo tumorigenicity assay. A comparative proteomic approach was performed to identify and clarify the underlying molecular mechanisms. SSX2 expression was more pronounced in ERα-negative breast cancer cells compared with the positive ones. Overexpression of SSX2 induced cell growth and prompted cell invasion. Both ERα and E-cadherin expression were suppressed in the SSX2 overexpressing MCF-7 cells. Eleven known proteins were identified with significant differential expression. Among these, five were decreased, while other six were increased in the SSX2 overexpressing MCF-7 cells. These results suggested SSX2 may enhance invasiveness in MCF-7 cells both in vivo and in vitro. The regulation of ERα signaling by target proteins of SSX2 may explain the metastatic potential of breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cancer/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ERα signaling

et al. 2012

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“…Transgelin is an actin cross-linking protein, which plays a key role in cell differentiation, cell migration cell invasion and matrix remodeling through stabilizing the cytoskeleton [5,6] . Daniel et al [7] reported that Transgelin is upregulated in repopulating mesangial cells and promotes their migratory.…”

Section: Discussionmentioning

confidence: 99%

“…Transgelin contains a C-terminal calponin-like module and an upstream positively charged amino acid region which is required for actin binding [4] . It is by stabilizing the cytoskeleton through binding to actin that Transgelin plays a role in cell differentiation, cell migration, cell invasion and matrix remodeling [5,6] . Transgelin is upregulated in repopulating mesangial cells, and promotes their migratory and proliferative repair response after injury [7] .…”

Section: Introductionmentioning

confidence: 99%

Effect of Knockdown TAGLN on the Migration Capacity of Wuzhishan Pig’s Bone Marrow Mesenchymal Stem Cells

Yang¹,

Tang²,

Liu³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryMesenchymal stem cells (MSCs) are multipotent adult stem cells, can easily be isolated from the bone marrow and subsequently expand in vitro. Bone marrow MSCs (BMMSCs) appear to be poorly immunogenic, and are the most widely used MSCs in tissue regenerative medicine, and the migration capacity of BMMSCs is the key determinant for the efficiency of their regenerative therapy. The Wuzhishan pig (WZSP) is characterized by its physiological and general biochemical indices very similar to humans, and may be potential organ donors for human being owing to its size. In this study, the BMMSCs from WZSP were used to study their migration capacity which regulated by TAGLN in vitro. The specific short hairpin RNA (shRNA) for TAGLN was designed to knock down TAGLN gene, and the scratch assay and transwell migration assay was employed to estimate effect of knockdown TAGLN on the migration capacity of BMMSCs from WZSP. The results showed that the specific shRNA for knocking down TAGLN efficiently was found for the BMMSCs, and there was a significant effect on the migration capacity of the BMMSCs from WZSP with knockdown TAGLN in vitro. In conclusion, TAGLN was an important factor in maintaining the migration capacity of the BMMSCs, which may be benefit for the BMMSCs from WZSP to be used in regenerative therapy for human being. Keywords: Bone marrow mesenchymal stem cells, Migration capacity, TAGLN, Wuzhishan pig TAGLN Geninin Bloke Edilmesinin Wuzhishan Domuz Kemik İliği Mezenkimal Kök Hücrelerinde Göç Etme Kapasitesi Üzerine Etkisi ÖzetMezenkimal kök hücreleri (MSCs) kemik iliğinden kolayca izole edilebilen ve takibinde in vitro olarak çoğalabilen mültipotent olgun kök hücreleridir. Kemik iliği MSC (BMMSC)'lerinin zayıf immunojenik özellikte olduğu görülmekte olup doku yenileme tedavisinde en sıklıkla kullanılan MSC'lerdir ve BMMSC'lerin göç etme kapasiteleri yenileme tedavisindeki başarının anahtarıdır. Wuzhishan domuzu (WZSP) fizyolojik ve genel biyokimyasal belirtileri açısından insana oldukça benzerdir ve boyutu düşünüldüğünde insana organ nakli bakımından oldukça yüksek potansiyele sahiptir. Bu çalışmada WZSP'den elde edilen BMMSC'lerin TAGLN tarafından düzenlenen göç kapasiteleri in vitro olarak çalışıldı. TAGLN için spesifik kısa hairpin RNA (shRNA) TAGLN genini bloke etmek için dizayn edildi. Scratch metodu ve transwell göç metodu bloke TAGLN genin WZSP'den elde edilen BMMSC'lerin göç kapasiteleri üzerine etkisini araştırmak amacıyla uygulandı. Sonuçlar TAGLN genini bloke etmede kullanılan spesifik shRNA'nın BMMSC için etkili olduğunu ve in vitro ortamda TAGLN blokajının WZSP'den elde edilen BMMSC'lerin göç kapasiteleri üzerine anlamlı bir etkisinin var olduğunu gösterdi. Sonuç olarak TAGLN BMMSC'lerde göç kapasitesini sağlamada önemli bir faktördür. Bu durum; WZSP'den elde edilen BMMSC'lerin insanlarda yenileme tedavilerinde kullanılmasına olanak sağlayabilir.

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“…Among these processes, several specific genes and/or proteins are aberrantly expressed. For example, matrix metalloproteinase-7 [5], cofilin-1 [6], transgelin (TAGLN) [7] and laminin-332 [8] that are involved in the organization of cytoskeletal architecture and thus play pivotal roles in cell migration during normal physiological and pathological processes, are found to be significantly upregulated in the eutopic endometria from women with endometriosis compared with those without endometriosis.…”

Section: Introductionmentioning

confidence: 99%

Expression of DJ-1 and mTOR in Eutopic and Ectopic Endometria of Patients with Endometriosis and Adenomyosis

Guo

Gao

et al. 2015

Gynecol Obstet Invest

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Objective: Endometrial cells may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of DJ-1 and phosphorylated mammalian target of rapamycin (p-mTOR) in ectopic and eutopic endometria of endometriosis and adenomyosis. Methods: Endometrial specimens were obtained from healthy non-menopausal women (n = 17) or patients with ovarian endometriotic cysts (n = 48) or adenomyosis (n = 30) during January 2011 to June 2012. The expressions of DJ-1 and p-mTOR were evaluated by immunohistochemistry and western blotting methods. Results: The expressions of DJ-1 and p-mTOR were significantly higher in the ectopic endometria than those in the eutopic endometria of endometriosis and adenomyosis patients or normal endometria (FDR < 0.05). DJ-1 expression was positively correlated with the p-mTOR expression no matter at endometriosis (r = 0.736, FDR < 0.001) or adenomyosis (r = 0.809, FDR < 0.001). Conclusion: DJ-1 protein may be involved in endometrial cells proliferation, migration and angiogenesis by modulating the PI3K/Akt/p-mTOR signaling pathway, which provides an underlying theoretical target for endometriosis and adenomyosis.

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TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation

Cited by 28 publications

References 31 publications

Cancer/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ERα signaling

Cancer/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ERα signaling

Effect of Knockdown TAGLN on the Migration Capacity of Wuzhishan Pig’s Bone Marrow Mesenchymal Stem Cells

Expression of DJ-1 and mTOR in Eutopic and Ectopic Endometria of Patients with Endometriosis and Adenomyosis

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