TAL effector RVD specificities and efficiencies

Streubel, Jana; Blücher, Christina; Landgraf, Angelika; Boch, Jens

doi:10.1038/nbt.2304

Cited by 267 publications

(325 citation statements)

References 11 publications

Supporting

Mentioning

305

Contrasting

Unclassified

Order By: Relevance

“…DOSSIER TECHNIQUE REVUES niers sont souvent préférés aux di-résidus NK et NH en raison d'une plus forte affinité pour leur cible [12,13]. La région de liaison à l'ADN des TALE se termine par la dernière répétition (LR, last repeat) qui n'est composée que de 20 résidus, mais possède toutefois les di-résidus variables qui assurent la spécificité de liaison à un nucléotide particulier.…”

Section: Talen Et Ingénierie Génomiqueunclassified

L’ingénierie des génomes par les TALEN

Dupret

Angrand

2014

Med Sci (Paris)

View full text Add to dashboard Cite

> La modification précise des génomes est un des enjeux majeurs des biotechnologies et de la recherche biomédicale. La découverte des TALE (transcription activator-like effector) et le développement récent des TALEN (transcription activator-like effector nuclease) artificiels offrent la possibilité d'éditer les génomes de nombreux organismes modèles de manière rapide, spécifique et efficace. Les TALEN sont des ciseaux moléculaires qui permettent d'induire différentes modifications génétiques en un site choisi du génome, et il est probable qu'ils deviennent, au cours des prochaines années, un des outils incontournables de la génétique inverse appliquée à la médecine. <

show abstract

Section: Talen Et Ingénierie Génomiqueunclassified

L’ingénierie des génomes par les TALEN

Dupret

Angrand

2014

Med Sci (Paris)

View full text Add to dashboard Cite

show abstract

“…Unlike zinc fingers, which recognize DNA triplets, each TALE repeat recognizes only a single bp, with little to no target site overlap from adjacent domains (Deng et al 2012;Mak et al 2012). The most commonly used RVDs for assembling synthetic TALE arrays are: NI for adenine, HD for cytosine, NG for thymine, and NN or HN for guanine or adenine (Boch et al 2009;Moscou and Bogdanove 2009;Cong et al 2012;Streubel et al 2012). TALE DNA-binding domains can be constructed using a variety of methods, with the most straightforward approach being Golden Gate assembly (Cermak et al 2011).…”

Section: Tale Nucleasesmentioning

confidence: 99%

Genome-Editing Technologies: Principles and Applications

Gaj

Sirk

Shui

et al. 2016

Cold Spring Harb Perspect Biol

297

142

View full text Add to dashboard Cite

Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the engineering advances that facilitated their development and highlight several achievements in genome engineering that were made possible by these tools. We also consider artificial transcription factors, illustrating how this technology can complement targeted nucleases for synthetic biology and gene therapy.

show abstract

“…The naturally occurring TALE repeats comprise tandem arrays with 10 to 30 repeats that bind and recognize extended DNA sequences (25). Each repeat is 33 to 35 amino acids in length, with two adjacent amino acids (termed the repeat-variable di-residue [RVD]) conferring specificity for one of the four DNA base pairs (26)(27)(28)(29)(30). Thus, there is a one-to-one antibiotic resistance cassette from the genome using systems such as Cre-lox and Flp-FRT are unnecessary, in contrast to traditional homologous recombination.…”

Section: Transcription Activator-like Effector Nucleasesmentioning

confidence: 99%