TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae

Sivaraman, Sruthi; Dineshkumar, Krishnamoorthy; Kumar, Arvind; Grace, Tony; Sharma, Jyotsana; Antony, Ginny

doi:10.1007/s00294-022-01232-4

Cited by 6 publications

(5 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integration of transcriptome analysis and EBE site prediction 54 has become a widely utilized strategy for identifying S genes targeted by Xanthomonas spp. TALEs 21,[56][57][58][59][60] . Through this approach, we identified the pectin lyase gene GhPL1 as an additional target of Tal7b, quantitively contributing to water-soaked symptom development, synergistically with GhSWEET14a and GhSWEET14b.…”

Section: Discussionmentioning

confidence: 99%

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Shan,

Mormile,

Bauer

et al. 2024

Preprint

View full text Add to dashboard Cite

Xanthomonas spp. employ transcription activator-like effectors (TALEs) to promote pathogenicity by activating host susceptibility (S) genes. Cotton GhSWEET10 is an S gene targeted by a TALE in an early isolate of Xanthomonas citri pv. malvacearum (Xcm), but not by recent field Xcm isolates. To understand the pathogenicity shift in Xcm and its adaptation to cotton, we assembled the whole genome and the TALE repertoire of three recent Xcm Texas field isolates. A newly evolved TALE, Tal7b, activated different GhSWEET genes, GhSWEET14a and GhSWEET14b. Simultaneous activation of GhSWEET14a and GhSWEET14b resulted in pronounced water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identified a pectin lyase as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9-based gene editing supported the function of GhSWEETs as S genes in cotton bacterial blight and the promise of disrupting the TALE-binding site in these genes to control the disease. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes simultaneously to promote pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Shan,

Mormile,

Bauer

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The integration of transcriptome analysis and EBE site prediction 54 has become a widely utilized strategy for identifying S genes targeted by Xanthomonas spp. TALEs 21, [56][57][58][59][60] . Through this approach, we identified the pectin lyase gene GhPL1 as an additional target of Tal7b, quantitively contributing to water-soaked symptom development, synergistically with GhSWEET14a and GhSWEET14b.…”

Section: Discussionmentioning

confidence: 99%

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Mormile,

Bauer,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Xanthomonasspp. employ transcription activator-like effectors (TALEs) to promote pathogenicity by activating host susceptibility (S) genes. CottonGhSWEET10is anSgene targeted by a TALE in an early isolate ofXanthomonas citripv.malvacearum(Xcm), but not by recent fieldXcmisolates. To understand the pathogenicity shift inXcmand its adaptation to cotton, we assembled the whole genome and the TALE repertoire of three recentXcmTexas field isolates. A newly evolved TALE, Tal7b, activated differentGhSWEETgenes,GhSWEET14aandGhSWEET14b. Simultaneous activation ofGhSWEET14aandGhSWEET14bresulted in pronounced water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identified a pectin lyase as an additional Tal7b target, quantitatively contributing toXcmvirulence alongsideGhSWEET14a/b. CRISPR-Cas9-based gene editing supported the function ofGhSWEETsasSgenes in cotton bacterial blight and the promise of disrupting the TALE-binding site in these genes to control the disease. Collectively, our findings elucidate the rapid evolution of TALEs inXanthomonasfield isolates and highlight the virulence mechanism wherein TALEs induce multipleSgenes simultaneously to promote pathogenicity.

show abstract

“…This data article presents the high-quality long-read genome sequence data of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae strain 119 [1] . Xcp -119 was isolated from a diseased pomegranate plant in Solapur, Maharashtra, India, and the whole genome sequencing was done with 100.8 X coverage using the single-molecule real-time long-read PacBio sequencing platform.…”

Section: Data Descriptionmentioning

confidence: 99%

Long-read genome sequence data of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae 119

2023

View full text Add to dashboard Cite

TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae

Cited by 6 publications

References 64 publications

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Long-read genome sequence data of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae 119

Contact Info

Product

Resources

About