Tamoxifen effects on the early life stages and reproduction of Japanese medaka (Oryzias latipes)

Sun, Liwei; Zha, Jinmiao; Spear, Philip A.; Wang, Zijian

doi:10.1016/j.etap.2007.01.003

Cited by 58 publications

(38 citation statements)

References 34 publications

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“…To our knowledge, only one paper has reported the significant alteration of genotypic sex ratio in fish exposed to tamoxifen. Sun et al (2007) found a significant increase in the proportion of F1 male O. latipes relative to the controls when breeding pairs medaka were exposed to anti-estrogen tamoxifen for 21 days. They think that the observed change in sex ratio may be caused by differential sensitivity of spermatozoa (i.e.…”

Section: Discussionmentioning

confidence: 97%

“…At a concentration of 5000 ng/L, E3 led to sex reversal of four males, which indicated that high concentrations of E3 can induce feminization of fish. Sex reversal in fish exposed to EDCs has been reported previously and may involve estrogenic chemicals eliciting female characteristics in males or androgenic chemicals producing male traits in females (Iwamatsu et al, 2000;Sun et al, 2007). Sex reversal of male fish is considered a consequence of the transformation from testes to ovaries during early gonadal development under the direct influence of environmental estrogens (Zha et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, males are orange-red and females are white (Edmunds et al, 2000). For Japanese medaka, the male or female fish in about 15 days post-hatch can be distinguished by color (Sun et al, 2007).…”

Section: Exposure and Experimental Designmentioning

confidence: 99%

“…Egg clutches were separated by gently rolling them with a finger. Eggs were disinfected by placing them in a 0.9% solution of hydrogen peroxide for 10 min, and then checked for fertilization using a dissecting microscope (Sun et al, 2007). The embryos were exposed to nominal E3 concentrations of 5, 50, 500 and 5000 ng/L in charcoal-dechlorinated tap water for 15 days.…”

Section: Exposure and Experimental Designmentioning

confidence: 99%

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Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000 ng/L E3 for 15 days, and the hatched fry were exposed continuously to the same concentrations for an additional 15 days. Exposure to 500 and 5000 ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000 ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000 ng/L E3 for further 60 days, the male hepatosomatic index (HSI) was increased at 5000 ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000 ng/L E3, while the male GSI at 5000 ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000 ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5 ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Exposure and Experimental Designmentioning

confidence: 99%

Section: Exposure and Experimental Designmentioning

confidence: 99%

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Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…However, coexposure to TAM could not neutralize the impaired reproductive capacity caused by E2, and complete reproductive failure occurred at the highest concentration (250 g/L). In our previous study (Sun et al, 2007a), exposure to TAM alone affected the reproductive capacity detrimentally at a concentration of only 625 g/L, but no paired medaka ceased spawning. Therefore, these findings demonstrate that combined exposure to E2 and TAM can impair the reproductive function more severely than exposure to each component alone.…”

Section: Discussionmentioning

confidence: 99%

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

2009

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a b s t r a c tIt is supposed that the estrogenic effects of estrogenic chemicals will be balanced by the presence of other endocrine-disrupting chemicals (EDCs) with different properties. However, little convincing research into this issue has been carried out to date. In the present study, the combined effects of EDCs with estrogenic and antiestrogenic activities were assessed using a 21-day reproduction assay with the Japanese medaka (Oryzias latipes) as an in vivo model. Natural estrogen (17␤-estradiol, E2) and the pharmaceutical substances letrozole and tamoxifen, which are both model antiestrogens, were tested as reference compounds individually and in combination. Exposure to E2 (200 ng/L) alone resulted in significant biological changes in paired fish, including impaired reproductive capacity and plasma vitellogenin induction. Upon coexposure with tamoxifen or letrozole (10, 50 and 250 g/L), the effects of estrogen on some biomarkers (e.g., plasma vitellogenin concentration) could be neutralized to some extent, but the impairments in reproductive performance were hardly ameliorated or even became more severe. Since reproductive performance is a more holistic parameter with population-level relevance, the possibility that the effects of estrogenic chemicals can be cancelled out by the presence of antiestrogens in aquatic organisms should be questioned. Our observations also illustrate the importance of considering multiple endpoints at different biological levels of organisms for accurate and adequate assessment of the integrated effects of EDCs, especially in environmentally relevant exposure situations.

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Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

Gharbavi,

Johari,

Tabar

et al. 2023

Applied Organom Chemis

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In this study, we aimed to improve the delivery and efficacy of the well‐known anticancer drug, tamoxifen (TAM), through its encapsulation in bovine serum albumin nanoparticles (BSA NPs) and their hybridization with selenium nanoparticles (Se NPs). The developed nanostructures were characterized by Fourier transform infrared (FT‐IR) spectroscopy, ultraviolet–visible (UV–Vis) spectroscopy, energy‐dispersive X‐ray spectroscopy (EDS) analysis, dynamic light scattering (DLS), and field emission scanning electron microscopy (FESEM) imaging. The impact of nanostructures on 4T‐1 cancer cells was assessed through MTT and flow cytometry assays to measure cytotoxicity and apoptosis rates, respectively. FT‐IR and UV–Vis spectroscopy confirmed the successful encapsulation of TAM within BSA NPs. EDS analysis confirmed the Se integration into BSA‐TAM@Se NPs, signifying successful hybridization. Size and zeta potential measurements revealed the average hydrodynamic size and surface charge of the nanostructures. BSA NPs, BSA@Se NPs, and Se NPs showed the sizes ranging from 153.37 ± 1.02 to 229.63 ± 1.36 nm, with zeta potential values from −10.12 ± 0.26 to −28.45 ± 0.40 mV. Hemocompatibility studies demonstrated minimal hemolytic activity of the nanostructures, indicating their biocompatibility for intravenous administration. Cellular toxicity assays showed higher cytotoxicity of BSA‐TAM@Se NPs compared to other formulations (****p < 0.0001). The increased cell death in 4T‐1 breast cancer cells highlights the enhanced therapeutic potential of BSA‐TAM@Se NPs. Apoptosis analysis revealed a significant increase in apoptosis rate in 4T‐1 cells treated with BSA‐TAM@Se NPs compared to other formulations (****p < 0.0001), emphasizing their ability to induce programmed cell death. These findings support the rational importance of BSA NPs as drug nanocarriers and their hybridization with Se NPs to improve therapeutic effects. Finally, we suggest that the developed BSA‐TAM@Se NPs can be used as a potential therapeutic approach in combination with other tools such as X‐ray irradiation in future studies.

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Tamoxifen effects on the early life stages and reproduction of Japanese medaka (Oryzias latipes)

Cited by 58 publications

References 34 publications

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

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