Tandem mass tag-based proteomics analysis reveals the effects of Guri Gumu-13 pill on drug-induced liver injury

Kang, Songsong; Yang, Bo; Yang, Dawei; Wu, Guodong; Yang, Xuemiao; Wei, Jinhui; Zhao, Guojun; An, Ming; Zhao, Longshan

doi:10.1016/j.jchromb.2022.123353

Cited by 4 publications

(3 citation statements)

References 63 publications

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“…The protective effect of Guri Gumu-13 pill on acetaminophen-induced liver injury was investigated based on proteomics. Total 237 proteins were identified and 58 differentially expressed proteins were regulated and therapeutic effects were related to linoleic acid and retinol metabolism regulation [32]. The effects and underlying mechanism of Tuomin-Zhiti-Decoction treating allergic rhinitis were explored by integrative analysis of proteomics and network pharmacology.…”

Section: Proteomicsmentioning

confidence: 99%

Multi-omics technology for demystifying herbal medicine

Zhang¹,

Qiu²

2023

Adv. Mater. Lett.

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Section: Proteomicsmentioning

confidence: 99%

Multi-omics technology for demystifying herbal medicine

Zhang¹,

Qiu²

2023

Adv. Mater. Lett.

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“…TMT-based proteomics analysis revealed that the Guri Gumu-13 pill exerts therapeutic effects through multipathway regulation in treating drug-induced liver injury (Kang et al, 2022)…”

Section: Introductionmentioning

confidence: 99%

“…Wu showed that quantitative proteomics was used to study the effect of Huangqi decoction on the nuclear factor (NF)‐κB/IL‐6/STAT3 signaling pathway to improve acute cholestatic liver injury (Wu et al, 2020). TMT‐based proteomics analysis revealed that the Guri Gumu‐13 pill exerts therapeutic effects through multipathway regulation in treating drug‐induced liver injury (Kang et al, 2022). Therefore, based on the results of PDPPAS and CAS polysaccharide (CASP) on chicken liver injury induced by CS combined with LPS, this study used TMT proteomics technology combined with MRM verification to analyze the intervention mechanism of CASP high‐dose (CASPH) on chicken liver injury induced by CS combined with LPS, providing a basis for the development of prevention and treatment drugs for chicken liver injury.…”

Section: Introductionmentioning

confidence: 99%

Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach

Li,

Ji,

et al. 2024

Biomedical Chromatography

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The incidence of colibacillosis in poultry is on the rise, significantly affecting the chicken industry. Ceftiofur sodium (CS) is frequently employed to treat this disease, resulting in lipopolysaccharide (LPS) buildup. Processing plays a vital role in traditional Chinese veterinary medicine. The potential intervention in liver injury by polysaccharides from the differently processed products of Angelica sinensis (PDPPAS) induced by combined CS and LPS remains unclear. This study aims to investigate the protective effect of PDPPAS on chicken liver injury caused by CS combined with LPS buildup and further identify the polysaccharides with the highest hepatoprotective activity in chickens. Furthermore, the study elucidates polysaccharides’ intervention mechanism using tandem mass tag (TMT) proteomics and multiple reaction monitoring (MRM) methods. A total of 190 1‐day‐old layer chickens were randomly assigned into 12 groups, of which 14 chickens were in the control group and 16 in other groups, for a 10‐day trial. The screening results showed that charred A. sinensis polysaccharide (CASP) had the most effective and the best hepatoprotective effect at 48 h. TMT proteomics and MRM validation results demonstrated that the intervention mechanism of the CASP high‐dose (CASPH) intervention group was closely related to the protein expressions of FCER2, TBXAS1, CD34, AGXT, GCAT, COX7A2L, and CYP2AC1. Conclusively, the intervention mechanism of CASPH had multitarget, multicenter regulatory features.

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