Tangential flow filtration: A method to concentrate freshwater algae

Petrus̆evski, B.; Bolier, G.; Breemen, A.N. Van; Alaerts, Guy J.

doi:10.1016/0043-1354(94)00269-d

Cited by 119 publications

(42 citation statements)

References 5 publications

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“…Because particle concentration was too low to generate a sufficient quantity of aggregates for the biological and chemical analysis, each of the field samples was enriched in particulate material: First, particles >0·16 m were concentrated from 20 l to approximately 13 l by tangential flow filtration (TFF). TFF is a gentle method for the concentration of plankton without significant damage to the cells structure or viability (Petruševski et al, 1995). One litre of filtrate (<0·16 m) was stored at 5 C for later resuspension of aggregates.…”

Section: Aggregation Experimentsmentioning

confidence: 99%

Chemical and Biological Composition of Suspended Particles and Aggregates in the Baltic Sea in Summer (1999)

Engel¹,

Meyerhöfer²,

Bröckel³

2002

Estuarine, Coastal and Shelf Science

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Suspended particles and particle aggregates, which formed from concentrated field samples on the roller table, were characterized biologically and chemically along a transect through the Baltic Sea in summer 1999. Phytoplankton composition in field samples was dominated by cyanobacteria, including the filamentous diazotrophic cyanobacteria Aphanizomenon ' baltica', Nodularia spumigena and Anabaena spp. These species formed aggregates together with diatoms, mainly Skeletonema costatum and Chaetoceros spp. and with dinoflagellates, mainly with Dinophysis norvegica. Compared to the Redfield ratio, concentration ratios of particulate organic carbon, nitrogen and phosphorus, [POC]: [PON]: [POP], indicated an enrichment of carbon, especially in aggregates. However, regression analysis indicated a higher production rate of PON relative to POP and POC and significant background concentrations of POC. In field samples the concentration of transparent exopolymer particles (TEP) varied around 200 g Xanthan Equiv. l 1 and comprised a volume fraction of 2-7 ppm and an abundance of about 10 5 TEP ml 1 . TEP were enriched in aggregates as inferred from volume ratios of TEP to conventional particles. It is suggested, that TEP contribute substantially to the background concentration of POC, while the high production rate of PON is attributed to nitrogen fixation of diazotrophic cyanobacteria.

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Section: Aggregation Experimentsmentioning

confidence: 99%

Chemical and Biological Composition of Suspended Particles and Aggregates in the Baltic Sea in Summer (1999)

Engel¹,

Meyerhöfer²,

Bröckel³

2002

Estuarine, Coastal and Shelf Science

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“…Samples for As were collected in acidwashed polyethylene bottles, and quick frozen to preserve As speciation. Suspended particles were concentrated from 8 l using a 500,000 NMWC cross-flow filtration cartridge, and collected on 0.4 m polycarbonate filters (Petruševski et al 1995;Wen et al 1996;Riedel et al 2000).…”

mentioning

confidence: 99%

The interrelationships among trace element cycling, nutrient loading, and system complexity in estuaries: A mesocosm study

Riedel¹,

Sanders

2003

Estuaries

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Biogeochemical interactions between a suite of trace elements and nutrients were examined in a series of experimental mesocosm experiments to understand how multiple stressors affect estuarine environments and how these effects are modified by the complexity of the system used to examine them. Experimental treatment included additions of nutrients and trace elements separately and combined, along with a gradient in experimental system complexity. Eight mesocosm experiments were carried out from 1996 through 1998. Increased nutrients generally decreased dissolved trace element concentrations, in large part through an increase in phytoplankton biomass, but also by increasing the concentration of metals in the particles. Trace element additions increased dissolved nutrients by decreasing phytoplankton biomass. The presence of sediments reduced both dissolved trace element and nutrient concentrations. Other complexity treatments had weaker effects on both dissolved nutrients and trace elements. Many of the observed effects appeared to be seasonal, occurring only in spring, or their magnitude was greater in spring. This may be linked to a change from phosphorus to nitrogen limitation that often occurs in the Patuxent River estuary in the late spring or early summer period.

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“…Using a tangential, turbulent flow of liquid across the membrane prevents clogging of the filter with cells. The efficiency of the process is very dependent on cell morphology and the transmembrane pressure (Petrusevski et al, 1995). A more unconventional approach is magnetic filtration.…”

Section: Harvesting Methodsmentioning

confidence: 99%

“…Conventional filtration is only effective for larger (> 70 m) or filamentous species such as Coelastrum and Spirulina (Brennan & Owende, 2010, Lee et al, 2009. For smaller cells, microfiltration, ultra-filtration and membrane-filtration can be used, though usually only for small volumes (Brennan & Owende, 2010, Petrusevski et al, 1995, Borowitzka, 1997. Fouling (accumulation of material on the surface of the membrane, slowing filtration) is a major problem.…”

Section: Harvesting Methodsmentioning

confidence: 99%

“…Morphological characteristics that influence harvesting include cell motility, size, shape, cell wall elongations such as spines and flagella, colony formation, and the presence of extracellular mucilage layers or capsules (Petrusevski et al, 1995;Jarvis et al, 2009). Larger particles allow for easier separation due to increased surface area and mass.…”

Section: Factors Affecting Separationmentioning

confidence: 99%

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