Target-Specific Expression of Presynaptic NMDA Receptors in Neocortical Microcircuits

Buchanan, Katherine A.; Blackman, Arne V.; Moreau, Alexandre; Elgar, Dale; Costa, Rui Ponte; Lalanne, Txomin; Jones, Adam A. Tudor; Oyrer, Julia; Sjöström, P. Jesper

doi:10.1016/j.neuron.2012.06.017

Cited by 126 publications

(227 citation statements)

References 51 publications

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“…Elegant work has provided evidence that NMDARs regulate presynaptic plasticity in the visual and somatosensory neocortex, entorhinal cortex, hippocampus, amygdala, cerebellum and spinal cord (Bardoni et al, 2004;Bender et al, 2006;Berretta and Jones, 1996;Brasier and Feldman, 2008;Buchanan et al, 2012;Casado et al, 2000;Casado et al, 2002;Corlew et al, 2007;Duguid and Smart, 2004;Fiszman et al, 2005;Glitsch and Marty, 1999;Humeau et al, 2003;Jourdain et al, 2007;Li and Han, 2007;Li et al, 2008;Liu et al, 1997;Mameli et al, 2005;Rodríguez-Moreno et al, 2011;Rodríguez-Moreno and Paulsen, 2008;Sjöström et al, 2003;Yang et al, 2006). At least in certain developing neurons, the responsible receptors are found in the presynaptic rather than in the postsynaptic neuron.…”

Section: Presynaptic Nmdarsmentioning

confidence: 99%

“…During cortical development, activation of NMDARs in presynaptic neurons facilitates neurotransmitter release and is required for spike-timing-dependent long-term depression (tLTD) (Berretta and Jones, 1996;Brasier and Feldman, 2008;Buchanan et al, 2012;Corlew et al, 2007;Rodríguez-Moreno and Paulsen, 2008;Sjöström et al, 2003). Although dendritic NMDARs might contribute to presynaptic plasticity (Christie and Jahr, 2008;Christie and Jahr, 2009), axonal NMDARs are necessary for tLTD (Rodríguez-Moreno et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Although dendritic NMDARs might contribute to presynaptic plasticity (Christie and Jahr, 2008;Christie and Jahr, 2009), axonal NMDARs are necessary for tLTD (Rodríguez-Moreno et al, 2011). In addition, Ca 2+ transients are observed in presynaptic boutons upon focal uncaging of NMDA or glutamate (Buchanan et al, 2012;McGuinness et al, 2010). However, it is not clear whether the responsible axonal receptors are distributed throughout the axon or whether they are enriched at nerve terminals (Buchanan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, Ca 2+ transients are observed in presynaptic boutons upon focal uncaging of NMDA or glutamate (Buchanan et al, 2012;McGuinness et al, 2010). However, it is not clear whether the responsible axonal receptors are distributed throughout the axon or whether they are enriched at nerve terminals (Buchanan et al, 2012). If they localize to presynaptic terminals, it is unknown which presynaptic structures contain NMDARs.…”

Section: Introductionmentioning

confidence: 99%

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Presynaptic NMDA receptors: dynamics and distribution in developing axons in vitro and in vivo

Gill

Droubi

Giovedì

et al. 2014

Journal of Cell Science

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BSTRACTDuring cortical development, N-methyl-D-aspartate (NMDA) receptors (NMDARs) facilitate presynaptic terminal formation, enhance neurotransmitter release and are required in presynaptic neurons for spike-timing-dependent long-term depression (tLTD). However, the extent to which NMDARs are found within cortical presynaptic terminals has remained controversial, and the subsynaptic localization and dynamics of axonal NMDARs are unknown. Here, using live confocal imaging and biochemical purification of presynaptic membranes, we provide strong evidence that NMDARs localize to presynaptic terminals in vitro and in vivo in a developmentally regulated manner. The NR1 and NR2B subunits (also known as GRIN1 and GRIN2B, respectively) were found within the active zone membrane, where they could respond to synaptic glutamate release. Surprisingly, NR1 also appeared in glutamatergic and GABAergic synaptic vesicles. During synaptogenesis, NR1 was mobile throughout axons -including growth cones and filopodia, structures that are involved in synaptogenesis. Upon synaptogenic contact, NMDA receptors were quickly recruited to terminals by neuroligin-1 signaling. Unlike dendrites, the trafficking and distribution of axonal NR1 were insensitive to activity changes, including NMDA exposure, local glutamate uncaging or action potential blockade. These results support the idea that presynaptic NMDARs play an early role in presynaptic development.

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Section: Presynaptic Nmdarsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Presynaptic NMDA receptors: dynamics and distribution in developing axons in vitro and in vivo

Gill

Droubi

Giovedì

et al. 2014

Journal of Cell Science

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“…4). Because their dendritic arbors are indistinguishable 4 , these two cell types otherwise appear highly similar ( Fig. 1e-f).…”

Section: Competing Financial Interestsmentioning

confidence: 98%

Neuronal morphometry directly from bitmap images

et al. 2014

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Ionotropic glutamate receptors: Which ones, when, and where in the mammalian neocortex

Hadžić

Jack

Wahle

2016

J of Comparative Neurology

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A multitude of 18 iGluR receptor subunits, many of which are diversified by splicing and RNA editing, localize to >20 excitatory and inhibitory neocortical neuron types defined by physiology, morphology, and transcriptome in addition to various types of glial, endothelial, and blood cells. Here we have compiled the published expression of iGluR subunits in the areas and cell types of developing and adult cortex of rat, mouse, carnivore, bovine, monkey, and human as determined with antibody- and mRNA-based techniques. iGluRs are differentially expressed in the cortical areas and in the species, and all have a unique developmental pattern. Differences are quantitative rather than a mere absence/presence of expression. iGluR are too ubiquitously expressed and of limited use as markers for areas or layers. A focus has been the iGluR profile of cortical interneuron types. For instance, GluK1 and GluN3A are enriched in, but not specific for, interneurons; moreover, the interneurons expressing these subunits belong to different types. Adressing the types is still a major hurdle because type-specific markers are lacking, and the frequently used neuropeptide/CaBP signatures are subject to regulation by age and activity and vary as well between species and areas. RNA-seq reveals almost all subunits in the two morphofunctionally characterized interneuron types of adult cortical layer I, suggesting a fairly broad expression at the RNA level. It remains to be determined whether all proteins are synthesized, to which pre- or postsynaptic subdomains in a given neuron type they localize, and whether all are involved in synaptic transmission. J. Comp. Neurol. 525:976-1033, 2017. © 2016 Wiley Periodicals, Inc.

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Target-Specific Expression of Presynaptic NMDA Receptors in Neocortical Microcircuits

Cited by 126 publications

References 51 publications

Presynaptic NMDA receptors: dynamics and distribution in developing axons in vitro and in vivo

Presynaptic NMDA receptors: dynamics and distribution in developing axons in vitro and in vivo

Neuronal morphometry directly from bitmap images

Ionotropic glutamate receptors: Which ones, when, and where in the mammalian neocortex

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