Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

“…Tailoring single-stranded DNA for specic identication based on the reported genetic information of SARS-CoV-2 has been reported in many papers. 58,96,[153][154][155][156] Isothermal rolling circle amplication (RCA) can be used to assist in electrochemical genosensor. RCA amplicons can hybridize with probes that have redox signals for the identication of N and S genes in SARS-CoV-2 (ref.…”

Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

“…In the literature, some works report the use of DNA/RNA for modifications of electrochemical sensors applied for the detection of the SARS-CoV-2 [ 28 , 71 , 72 ]. Table S8 presents a comparison between the data obtained from the proposed genosensor with others presented in the literature for the detection of SARS-CoV-2.…”

“…The most reported sensors are screen-printed electrodes [ 71 , 73 ]. However, gold electrode [ 72 ], flex-printed circuit board (graphene-based) [ 74 ], platinum/titanium interdigitated electrodes on a glass substrate [ 16 ], in addition to one 3D printed sensor obtained using a 3D printing pen [ 28 ] can also be found in the literature. Among these works, the most varied stages of production of biosensors can be observed, reaching times greater than 3 h. Furthermore, LOD values ranging from 33.0 fmol L −1 to 0.1 µmol L −1 are observed.…”

An electrochemical biosensor for SARS-CoV-2 detection via its papain-like cysteine protease and the protease inhibitor screening

“…At present, various approaches for SARS-CoV-2 diagnosis have been proposed, which are mainly focused on the detection of SARS-CoV-2 nucleic acid or antibodies [6] , [7] , [8] , [9] , [10] . As the gold standard for nucleic acid detection, real-time quantitative polymerase chain reaction (RT-qPCR) has extremely high sensitivity and specificity, but it has inevitable limitations such as high equipment requirement, time-consumption, and professional technician requirement [11] , [12] .…”

“…Over the past period, some studies have also been conducted on SARS-CoV-2 detection methods in our laboratory, through nucleic acids [9] , [10] , [40] and spike proteins [26] . Based on these, we would like to avoid the complicated steps of nucleic acid extraction and improve the practical value of the detection method.…”