Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface

Abstract

Herein, we introduce the fabrication of polymer micropattern arrays on a chemically inert poly(dimethylsiloxane) (PDMS) surface and employ them for the selective adhesion of cells. To fabricate the micropattern arrays, a mercapto-ester-based photocurable adhesive was coated onto a mercaptosilane-coated PDMS surface and photopolymerized using a photomask to obtain patterned arrays at the microscale level. Robust polymer patterns, 380 microm in diameter, were successfully fabricated onto a PDMS surface, and cells were selectively targeted toward the patterned regions. Next, the performance of the cell adhesion was observed by anchoring cell adhesive linker, an RGD oligopeptide, on the surface of the mercapto-ester-based adhesive-cured layer. The successful anchoring of the RGD linker was confirmed through various surface characterizations such as water contact angle measurement, XPS analysis, FT-IR analysis, and AFM measurement. The micropatterning of a photocurable adhesive onto a PDMS surface can provide high structural rigidity, a highly-adhesive surface, and a physical pathway for selective cell adhesion, while the incorporated polymer micropattern arrays inside a PDMS microfluidic device can serve as a microfluidic platform for disease diagnoses and high-throughput drug screening.

A review on techniques to fabricate silicon oxide arrays for biomolecules patterning

“…UV photolithography is commonly used to fabricate groove and rise by exposing the photo resist over the substrate to UV through a mask to transfer its pattern on the substrate (followed by etching or uniform metal coatings) [23][24][25]. Commercial UV masks are available, but it is a tedious procedure to positioning the masks.…”

“…Tang et al [25] and Onoe and Takeuchi [28] have studied the cell interaction on ordered SiO 2 patterns. The whole cells were cultured from suitable media and then the whole cells are allowed for adherence on the micro patterns with suitable conditions and incubation time.…”

“…There are many methods to fabricate well controlled nanostructures, such as oxidation [7][8][9][10][11][12][13][14][15][16][17][18][19], laser [20][21][22], ultraviolet [23][24][25], etching [20,22], and imprint [26,27]. We are working on fabrication of silicon oxide using AFM anodic oxidation and a copper wire assisted homemade setup.…”

Contactless Laser-Assisted Patterning of Surfaces for Bio-Adhesive Microarrays

“…Even though certain progress have been made on the manufacturing of 3D structures and performing cell culture therein, most of the in vitro bioassays are still 2D due to the wealth of 2D analytical techniques available [2][3][4][5]. In order to gain meaningful insights in the processes of cell proliferation, differentiation as well as cellsurface interactions, a variety of 2D multimaterial engineered surfaces have been developed, so that suppressing non-specific adsorption in certain regions, cell orientation and growth can be studied [6][7][8][9]. In such approaches, cell adhesion is controlled by incorporating certain moieties that favour biocompatibility while minimizing adsorption of other biomolecules present in the cell growth media [10][11][12][13].…”

Device for oxide dots fabrication with copper wire as cathode probe

“…A self assembled uniform monolayer was formed on the surface by silanization process to increase the electrostatic interaction for anchoring biomolecules and no markable changes was found due to the uniform monolayer in SEM image (Fig. S2) (Jun et al 2006;Kumaran et al 2011;Tang et al 2010). SEM and AFM images (Fig.…”