Targeted delivery of antimicrobial peptide by Cry protein crystal to treat intramacrophage infection

Yang, Zaofeng; Zheng, Jiale; Chan, Chin-Fung; Wong, Iris L. K.; Heater, Bradley S.; Chow, Larry M.C.; Lee, Marianne M.M.; Chan, Michael K.

doi:10.1016/j.biomaterials.2019.119286

Cited by 38 publications

(56 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously reported the advantages of the Cry3Aa platform in the immobilization of enzymes and for the delivery of antimicrobial peptides due to its exceptional stability (Heater et al, 2018(Heater et al, , 2019Nair et al, 2015;Yang et al, 2019). Herein we demonstrated the utilization of the crystal-forming domain of Cry3Aa protein to directly produce 3ADI-Mb protein particles in Bt cells for myoglobin delivery.…”

Section: Discussionmentioning

confidence: 97%

“…One important feature of any drug delivery system, particularly for peptide-or protein-based therapeutics, is the ability of the carriers to confer protection to their cargos. We have previously demonstrated that the framework of Cry3Aa could enhance the stability of its protein cargo (Heater et al, 2018(Heater et al, , 2019Nair et al, 2015;Yang et al, 2019). We therefore hypothesized that the 3ADI-Mb particle should also be able to stabilize its fused Mb partner.…”

Section: Production and Characterization Of 3a-mb And 3adi-mbmentioning

confidence: 99%

“…Given the promising results obtained for 3ADI-GFP-A549 particles, we thus proceeded to modify 3ADI-Mb similarly to generate a 3ADI-Mb-A549 construct with potentially enhanced selectivity toward A549 cells. This construct was further modified for cell internalization since previous studies from our laboratory had shown that the related Cry3Aa protein crystals could not be easily taken up by non-phagocytic cells (Yang et al, 2019). Hence, the known cell penetrating peptide TAT (Frankel and Pabo, 1988;Green and Loewenstein, 1988) was introduced upstream of the targeting peptide A549.1 to generate the 3ADI-Mb-TATA549 construct (Figure S1).…”

Section: Modification Of 3adi-mb With Functional Peptidesmentioning

confidence: 99%

“…We have previously described a protein/peptide delivery platform based on Cry3Aa protein that naturally forms sub-micrometer-sized crystalline inclusions within the bacterium Bacillus thuringiensis (Bt) (Nair et al, 2015;Yang et al, 2019). This Cry platform has shown to considerably improve the stability of its cargo protein in the form of Cry3Aa-cargo fusion protein crystal (Heater et al, 2018(Heater et al, , 2019Nair et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Production and Characterization Of 3a-mb And 3adi-mbmentioning

confidence: 99%

Section: Modification Of 3adi-mb With Functional Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The mechanism of antibacterial action is related to their ability to adjust membrane permeability, which destructs the membrane structure of the pathogen. One particular property that makes AMPs effective towards multidrug‐resistant bacteria strains (Yang et al, 2019) is in its wide‐scale multitargeted action (Joo et al, 2016; Zharkova et al, 2019). Usually, AMPs exhibit a net positive charge with a high ratio of hydrophobic amino acids that allows peptides to selectively bind to negatively charged bacterial membranes (Lei et al, 2019; Zhen et al, 2019) to either disrupt the membrane (Kumar et al, 2018), or to enter the bacterium and inhibit intracellular functions (Yazici et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Next‐generation Antimicrobial Peptides (AMPs) incorporated nanofibre wound dressings

et al. 2020

View full text Add to dashboard Cite

The skin is the most exposed organ and, therefore, vulnerable to injury and wounds (Nguyen & Soulika, 2019). Wound healing is a complex tissue repair process, and failing to manage it could result in the formation of scars (Landén et al., 2016; Takeo et al., 2015). Tissue repair involves the partial tissue regeneration involving restitution of tissue components during the wound healing process (Atkin et al., 2019; Gonzalez et al.., 2016). Wound healing is a dynamic process consisting of four phases: inflammation, proliferation,

show abstract

Intrabacterial Nitroreductase‐Activated Imaging and Persistently Illuminated Photodynamic Therapy for Intracellular Infection Theranostics

Ran,

Cao,

Zheng

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Intracellular bacterial (ICB) infection is one of the most life‐threatening cases of drug resistance, and photodynamic therapy (PDT) is challenged by limited light penetration into tissues and unspecific toxicity to host cells. Herein, theranostic nanoparticles (NPs) are developed to facilitate on‐demand activation of photosensitizers inside ICBs and persistent PDT of deep‐tissue ICB infections. Mechanoluminescent SrAl2O4:Eu2+ and persistence luminescent CaS:Eu2+ are sequentially deposited on mesoporous silica to prepare mSC, followed by encapsulation by bacterial membranes with loaded nitroreductase (NTR)‐activatable methylene blue (nMB) to obtain mSC‐nMB@BM NPs. The fluorescence quenching of nMB with mSC offers few cytotoxicities even under ultrasonication, and bacterial membrane coatings mediate the specific NP internalization into ICB‐infected macrophages. Bacterial pore‐forming toxins trigger nMB release from NPs, and intrabacterial NTR rejuvenates methylene blue probes from nMB for real‐time imaging of ICB infections. Ultrasonication of NPs continuously excites the rejuvenated methylene blue to generate reactive oxidative species and reduce viable ICBs by 3.54 log magnitude folds. NP treatment on ICB‐infected mice exhibits full survival and restores viscera functions without any pathological abnormality. Therefore, this concise NP design demonstrates capabilities of bacterial membrane‐mediated specific internalization, bacterial toxin‐triggered release and intrabacterial NTR‐rejuvenation of fluorescent probes, and persistent internal light‐illumination for ICB theranostics.

show abstract

Targeted delivery of antimicrobial peptide by Cry protein crystal to treat intramacrophage infection

Cited by 38 publications

References 54 publications

Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation

Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation

Next‐generation Antimicrobial Peptides (AMPs) incorporated nanofibre wound dressings

Intrabacterial Nitroreductase‐Activated Imaging and Persistently Illuminated Photodynamic Therapy for Intracellular Infection Theranostics

Contact Info

Product

Resources

About