Targeted delivery using peptide-functionalised gold nanoparticles to white adipose tissues of obese rats

Thovhogi, Ntevheleni; Sibuyi, Nicole Remaliah Samantha; Meyer, Mervin; Onani, Martin O.; Madiehe, Abram Madimabe

doi:10.1007/s11051-015-2904-x

Cited by 41 publications

(61 citation statements)

References 36 publications

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“…Similarly, pentapeptide-GNPs (CALNN and others) concentrated in the liver following intravenous injection in rats, again without any signs of toxicity 92 . Other in vivo studies conducted using cyclic-RGD-conjugated nanogold tripods 93 , RGD-gold nanorods 94 , VEGF-receptor binding peptide- 95 and adipose homing peptidefunctionalised GNPs 96 similarly did not reveal any evidence of toxicity or other adverse effects, although it should be pointed out that all these studies are relatively short-term. An in vivo study in mice of unfunctionalised GNPs indicated significant toxicity of nanoparticles in the size range 8-37nm however, interestingly, conjugation of immunogenic peptides to the GNPs reduced toxicity significantly 97 .…”

Section: Discussionmentioning

confidence: 87%

Peptide-functionalized gold nanoparticles: versatile biomaterials for diagnostic and therapeutic applications

2017

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Colloidal gold solutions have been used for centuries in a wide variety of applications including staining glass and in the colouring of ceramics. More recently, gold nanoparticles (GNPs) have been studied extensively due to their interesting size-dependent electronic and optical properties. GNPs can be functionalized easily with biomolecules that contain thiols, amines, or even phosphine moieties. For example, the reaction of thiol-containing peptides with GNPs has been used extensively to prepare novel hybrid materials for biomedical applications. A range of different types of peptides can be used to access biomaterials that are designed to perform a specific role such as cancer cell targeting. In addition, specific peptide sequences that are responsive to external stimuli (e.g. temperature or pH) can be used to stabilise / destabilise the aggregation of colloidal GNPs. Such systems have exciting potential applications in the field of colorimetric sensing (including bio-sensing) and in targeted drug delivery platforms. In this review, we will give an overview of the current methods used for preparing peptide functionalized GNPs, and we will discuss their key properties outlining the 2 various applications of this class of biomaterial. In particular, the potential applications of peptide functionalized GNPs in areas of sensing and targeted drug delivery will be discussed.

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Section: Discussionmentioning

confidence: 87%

Peptide-functionalized gold nanoparticles: versatile biomaterials for diagnostic and therapeutic applications

2017

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“…16 Since this discovery, PHB was used as a vascular marker for obesity. Independent studies confirmed that molecules attached to AHP with amino acid sequence CKGGRAKDC are capable of targeting PHB expressed on the surface of ECs in the WAT of obese mice, 16,84 rats, 85 and monkeys. 86 The role of PHB in the regulation of biological processes leading to the development of diseases implicates PHB as a putative target in drug discovery.…”

Section: Phb and Its Role In Obesitymentioning

confidence: 92%

“…16,77,78,87 Targeted nanotherapy is currently being explored as a potential treatment for obesity in a rodent and non-human primate models of diet-induced obesity, by targeting the vascular system that supports the growth of hypertrophic adipocytes. 17,18,78,85 Disease-associated biomarkers play a crucial role in targeted drug delivery, tissue imaging, and diagnostics. Biomarkers are able to discriminate between a physiological and the pathological state of an organism.…”

Section: Phb and Its Role In Obesitymentioning

confidence: 99%

“…18,84,108 Figure 3 summarizes the initial steps toward developing a successful vascular targeted nanotherapy, in order to assess the feasibility of the strategy for the treatment of obesity as demonstrated by independent studies. [16][17][18]84,85 Targeted nanotherapy can be developed by attaching a targeting peptide and a therapeutic peptide onto the NPs, the NPs serve as drug carriers. The biological effect of the NP-conjugates is validated in vitro and in vivo before they can be considered for clinical use.…”

Section: Potential Application Of Nanotechnology In Obesitymentioning

confidence: 99%

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Vascular targeted nanotherapeutic approach for obesity treatment

Sibuyi¹,

Meyer²,

Onani³

et al. 2018

IJN

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Obesity is a global epidemic that poses a serious health concern due to it being a risk factor for life-threatening chronic diseases, such as type 2 diabetes, cancer, and cardiovascular diseases. Pharmacotherapy remains the mainstay for the management of obesity; however, its usefulness is limited due to poor drug efficacy, non-specificity and toxic side effects. Therefore, novel approaches that could provide insights into obesity and obesity-associated diseases as well as development of novel anti-obesity treatment modalities or improvement on the existing drugs are necessary. While the ideal treatment of obesity should involve early intervention in susceptible individuals, targeted nanotherapy potentially provides a fresh perspective that might be better than the current conventional therapies. Independent studies have shown improved drug efficacy by using prohibitin (PHB)-targeted therapy in obese rodents and non-human primates, thus providing a proof of concept that targeted nanotherapy can be a feasible treatment for obesity. This review presents a brief global survey of obesity, its impact on human health, its current treatment and their limitations, and the role of angiogenesis and PHB in the development of obesity. Finally, the role and potential use of nanotechnology coupled with targeted drug delivery in the treatment of obesity are discussed.

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“…Thovhogi et al functionalized GNPs with a synthetic adipose homing peptide (AHP) for targeted delivery to the WAT of rats with obesity. Compared to rats treated with GNPs alone, those treated with AHP‐GNPs had a much higher concentration of Au in WATs . Although in vivo degradation and toxicity‐related experiments of AHP‐GNPs were not mentioned in this research, it is a promising platform for targeted delivery of antiobesity therapeutics using gold nanoparticles with good loading capacity.…”

Section: Nanotechnology As a Treatment For Obesitymentioning

confidence: 94%

Nanotechnology‐Mediated Drug Delivery for the Treatment of Obesity and Its Related Comorbidities

Tsou

Wang

et al. 2019

Adv Healthcare Materials

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Obesity is a serious health issue affecting humanity on a global scale. Recognized by the American Medical Association as a chronic disease, the incidence of obesity continues to grow at an accelerating rate and obesity has become one of the major threats to human health. Excessive weight gain is tied to metabolic syndrome, which is shown to increase the risk of chronic diseases, such as heart disease and type 2 diabetes, taxing an already overburdened healthcare system and increasing mortality worldwide. Available treatments such as bariatric surgery and pharmacotherapy are often accompanied by adverse side effects and poor patient compliance. Nanotechnology, an emerging technology with a wide range of biomedical applications, has provided an unprecedented opportunity to improve the treatment of many diseases, including obesity. This review provides an introduction to obesity and obesity‐related comorbidities. The most recent developments of nanotechnology‐based drug delivery strategies are highlighted and discussed. Additionally, challenges and consideration for the development of nanoformulations with translational potential are discussed. The overall objective of this review is to enhance the understanding of the design and development of nanomedicine for treatments of obesity and related comorbidities.

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Targeted delivery using peptide-functionalised gold nanoparticles to white adipose tissues of obese rats

Cited by 41 publications

References 36 publications

Peptide-functionalized gold nanoparticles: versatile biomaterials for diagnostic and therapeutic applications

Peptide-functionalized gold nanoparticles: versatile biomaterials for diagnostic and therapeutic applications

Vascular targeted nanotherapeutic approach for obesity treatment

Nanotechnology‐Mediated Drug Delivery for the Treatment of Obesity and Its Related Comorbidities

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