Targeted Expression of Human <b>
                     <i>MYCN</i>
                  </b> Selectively Causes Pancreatic Neuroendocrine Tumors in Transgenic Zebrafish

Yang, Hong; Kutok, Jeffery L.; Lee, Nam Hyuk; Piao, Hui Ying; Fletcher, Christopher D.�M.; Kanki, John P.; Look, A. Thomas

doi:10.1158/0008-5472.can-04-0931

Cited by 81 publications

(44 citation statements)

References 41 publications

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“…MYCN overexpression, under the control of the myoD promoter, resulted in formation of pancreatic neuroendocrine tumors that originated from insulin-producing islet cells (Yang et al, 2004). In another study, conditional overexpression of activated human Ras (kRASG12D), under the control of ubiquitous b-actin promoter, resulted in several tumor types including rhabdomyosarcoma, intestinal hyperplasia and malignant peripheral nerve sheath tumors (Patton et al, 2005).…”

Section: Transgenic Linesmentioning

confidence: 99%

Catch of the day: zebrafish as a human cancer model

2008

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Zebrafish are making big waves in the field of cancer research. The effect has been widespread and continues to gain speed as more and more cancer researchers ride the wave of zebrafish biology. This has been largely due to the development of transgenic and xenograft models of cancer, which recapitulate many aspects of different human cancers including lymphoblastic T-cell leukemia, pancreatic cancer, melanoma and rhabdomyosarcoma. These models are already being utilized by academia and industry to search for genetic and chemical modifiers of cancer with success. The attention has been further stimulated by the amenability of zebrafish to pharmacological testing and the superior imaging properties of fish tissues that allow visualization of cancer progression and angiogenesis in live animals. This review summarizes the current zebrafish models of cancer and discusses their utility in human cancer research and future directions in the field.

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Section: Transgenic Linesmentioning

confidence: 99%

Catch of the day: zebrafish as a human cancer model

2008

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“…A number of chemical carcinogens potent in mammals seemed also to be efficient in induction of zebrafish tumors (3)(4)(5)(6)(7). As in rodent models, transgenic zebrafish that overexpress c-myc and N-myc oncogenes develop T-cell leukemia (8) and neuroendocrine tumors (9), respectively. These data indicate a close similarity between mechanisms of carcinogenesis in mammals and zebrafish.…”

Section: Introductionmentioning

confidence: 99%

Transplantable Tumor Lines Generated in Clonal Zebrafish

Mizgireuv¹,

Revskoy

2006

Cancer Research

113

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Transplantable zebrafish tumors are a novel and very promising model in cancer research. However, further progress in this field has been contained by a lack of true inbred lines in zebrafish. To overcome this problem, we generated two lines of homozygous diploid clonal zebrafish lines (i.e., CB1 and CW1), which allowed us to carry out transplantation of any tissue, including tumors, from one fish to another within a line without rejection of the graft. The primary tumors in CB1 fish were induced by N-nitrosodiethylamine (DEN). The histologic analysis of these tumors revealed different types of hepatocellular carcinomas, hepatoblastomas, hepatoma, cholangiocarcinoma, and pancreatic carcinoma. Four spontaneous acinar cell carcinomas of pancreas were also found in 10-to 18-month-old CB1 fish. Small pieces of tissue or cell suspensions of either DEN-induced or spontaneous tumors were serially transplanted into the peritoneal cavity of syngeneic fish at different stages of development from 5-day-old larvae to adult fish. The development of grossly visible tumors occurred from 2 weeks to 3 months after tumor grafting and grew either as solitary smooth nodules or as an amorphous jelly-like mass infiltrating abdominal organs. The majority of tumors were also successfully transplanted to isogeneic (F1 generation from crossing CB1 Â CW1) fish. At the present time, 19 transplantable zebrafish tumor lines have been generated and maintained for as long as 3 to 25 passages. This model provides a novel tool for studying experimental tumor biology and therapy and will become a cost effective system for high throughput screening of anticancer drugs. (Cancer Res 2006; 66(6): 3120-5)

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“…To capitalize on the powerful developmental and genetic tools available in zebrafish, researchers have created numerous zebrafish tumor models (Langenau et al, 2003Yang et al, 2004;Berghmans et al, 2005;Patton et al, 2005;Haramis et al, 2006;Sabaawy et al, 2006;Le et al, 2007). The ultimate goal of the majority of this work is to identify pathways that modify disease states.…”

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confidence: 99%

Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

et al. 2008

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The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.

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Targeted Expression of Human MYCN Selectively Causes Pancreatic Neuroendocrine Tumors in Transgenic Zebrafish

Cited by 81 publications

References 41 publications

Catch of the day: zebrafish as a human cancer model

Catch of the day: zebrafish as a human cancer model

Transplantable Tumor Lines Generated in Clonal Zebrafish

Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish

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