Targeted Gene Silencing Using RGD-Labeled Chitosan Nanoparticles

Han, Hee Dong; Mangala, Lingegowda S.; Lee, Jeong Won; Shahzad, Mian M.K.; Kim, Hye Sun; Shen, De-yu; Nam, Eun Ji; Mora, Edna; Stone, Rebecca L.; Lü, Chunhua; Lee, Sun Joo; Roh, Ju-Won; Nick, Alpa M.; López-Berestein, Gabriel; Sood, Anil K.

doi:10.1158/1078-0432.ccr-10-0005

Cited by 247 publications

(160 citation statements)

References 49 publications

Supporting

Mentioning

160

Contrasting

Order By: Relevance

“…The Arg-Gly-Asp (RGD) peptides are known for specific binding to a wide number of surface integrins, including α v β 3 , α 3 β 1 , and α 5 β 1 integrins (45)(46)(47). The good selectivity of RGD to cancer cells has been reported by numerous studies for delivering nanoparticles to cancer cells or tumors (48).…”

Section: Significancementioning

confidence: 99%

Targeting cancer cell integrins using gold nanorods in photothermal therapy inhibits migration through affecting cytoskeletal proteins

Ali

Tang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

165

113

View full text Add to dashboard Cite

Metastasis is responsible for most cancer-related deaths, but the current clinical treatments are not effective. Recently, gold nanoparticles (AuNPs) were discovered to inhibit cancer cell migration and prevent metastasis. Rationally designed AuNPs could greatly benefit their antimigration property, but the molecular mechanisms need to be explored. Cytoskeletons are cell structural proteins that closely relate to migration, and surface receptor integrins play critical roles in controlling the organization of cytoskeletons. Herein, we developed a strategy to inhibit cancer cell migration by targeting integrins, using Arg-Gly-Asp (RGD) peptide-functionalized gold nanorods. To enhance the effect, AuNRs were further activated with 808-nm near-infrared (NIR) light to generate heat for photothermal therapy (PPTT), where the temperature was adjusted not to affect the cell viability/proliferation. Our results demonstrate changes in cell morphology, observed as cytoskeleton protrusions-i.e., lamellipodia and filopodia-were reduced after treatment. The Western blot analysis indicates the downstream effectors of integrin were attracted toward the antimigration direction. Proteomics results indicated broad perturbations in four signaling pathways, Rho GTPases, actin, microtubule, and kinases-related pathways, which are the downstream regulators of integrins. Due to the dominant role of integrins in controlling cytoskeleton, focal adhesion, actomyosin contraction, and actin and microtubule assembly have been disrupted by targeting integrins. PPTT further enhanced the remodeling of cytoskeletal proteins and decreased migration. In summary, the ability of targeting AuNRs to cancer cell integrins and the introduction of PPTT stimulated broad regulation on the cytoskeleton, which provides the evidence for a potential medical application for controlling cancer metastasis.gold nanorods | cytoskeleton | integrin | metastasis | plasmonic photothermal therapy

show abstract

Section: Significancementioning

confidence: 99%

Targeting cancer cell integrins using gold nanorods in photothermal therapy inhibits migration through affecting cytoskeletal proteins

Ali

Tang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

165

113

View full text Add to dashboard Cite

show abstract

“…RGD labeled chitosan nanoparticles was fabricated for targeted silencing of multiple growth-promoting genes (Han et al, 2010). The RGD and chitosan were conjugated by thiolation reaction using Nsuccinimidyl 3-(2-pyridyldithio)-propionate.…”

Section: Chitosanmentioning

confidence: 99%

Polymers in Small-Interfering RNA Delivery

Singha

Namgung

Kim

2011

Nucleic Acid Therapeutics

185

112

View full text Add to dashboard Cite

This review will cover the current strategies that are being adopted to efficiently deliver small interfering RNA using nonviral vectors, including the use of polymers such as polyethylenimine, poly(lactic-co-glycolic acid), polypeptides, chitosan, cyclodextrin, dendrimers, and polymers-containing different nanoparticles. The article will provide a brief and concise account of underlying principle of these polymeric vectors and their structural and functional modifications which were intended to serve different purposes to affect efficient therapeutic outcome of small-interfering RNA delivery. The modifications of these polymeric vectors will be discussed with reference to stimuli-responsiveness, target specific delivery, and incorporation of nanoconstructs such as carbon nanotubes, gold nanoparticles, and silica nanoparticles. The emergence of small-interfering RNA as the potential therapeutic agent and its mode of action will also be mentioned in a nutshell.

show abstract

“…A study shows that RGD-CH-NP is a novel and highly selective delivery system for siRNA with the potential for broad applications in human disease. 43 RGD peptide conjugated with chitosan by thiolation reaction was confirmed by proton-nuclear magnetic resonance (H-NMR). Binding of RGD-CH-NP with alpha(5)beta(3) integrin was examined by flow cytometry and fluorescence microscopy.…”

Section: Dovepress Dovepressmentioning

confidence: 98%

Nanomedicine: towards development of patient-friendly drug-delivery systems for oncological applications

Ranganathan¹,

Madanmohan²,

Kesavan³

et al. 2012

IJN

View full text Add to dashboard Cite

The focus on nanotechnology in cancer treatment and diagnosis has intensified due to the serious side effects caused by anticancer agents as a result of their cytotoxic actions on normal cells. This nonspecific action of chemotherapy has awakened a need for formulations capable of definitive targeting with enhanced tumor-killing. Nanooncology, the application of nanobiotechnology to the management of cancer, is currently the most important area of nanomedicine. Currently several nanomaterial-based drug-delivery systems are in vogue and several others are in various stages of development. Tumor-targeted drug-delivery systems are envisioned as magic bullets for cancer therapy and several groups are working globally for development of robust systems.

show abstract

Targeted Gene Silencing Using RGD-Labeled Chitosan Nanoparticles

Cited by 247 publications

References 49 publications

Targeting cancer cell integrins using gold nanorods in photothermal therapy inhibits migration through affecting cytoskeletal proteins

Targeting cancer cell integrins using gold nanorods in photothermal therapy inhibits migration through affecting cytoskeletal proteins

Polymers in Small-Interfering RNA Delivery

Nanomedicine: towards development of patient-friendly drug-delivery systems for oncological applications

Contact Info

Product

Resources

About