Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation

Palumbo, Giuseppe; Caruso, Martina; Crescenzi, Elvira; Tecce, Mario Felice; Roberti, G.; Colasanti, Alberto

doi:10.1016/s1011-1344(96)07335-6

Cited by 95 publications

(82 citation statements)

References 20 publications

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“…Depending on the wavelength and pulse duration, the membrane permeation mechanism varies as does the required dose and achievable optoinjection, transfection efficiency and cell viability. In terms of continuous wave lasers, a blue laser can be used for both stable [3] and transient [4] DNA phototransfection, as well as interfering RNA mediated gene silencing [5], though it is currently less mature and proven than the use of short pulse lasers.…”

Section: Biophotonicsmentioning

confidence: 99%

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Antkowiak

Torres-Mapa

Gunn‐Moore

et al. 2010

Journal of Biophotonics

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We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for the controlled and enhanced optoinjection and phototransfection of mammalian cells with a femtosecond light source. The SLM provides full control over the lateral and axial positioning of the beam with sub‐micron precision. Fast beam translation enables time‐sequenced irradiation, which is shown to enhance the optoinjection efficiency and alleviate the problem of exact beam positioning on the cell membrane. We show that irradiation in three axial positions doubles the number of viably optoinjected cells when compared with a single dose. The presented system also enables untargeted raster scan irradiation which provides a higher throughput transfection of adherent cells at the rate of 1 cell per second. Additionally, fluorescent imaging is used to demonstrate cell selective two‐step gene therapy. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Biophotonicsmentioning

confidence: 99%

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Antkowiak

Torres-Mapa

Gunn‐Moore

et al. 2010

Journal of Biophotonics

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“…Other studies of laser-induced gene delivery were carried out on single cells. [8][9][10][11] Our results, that laser engery is capable of enhancing gene transfer as well in cell suspensions, are promising for future in vivo laser application in the prostate. James et al 14 considered that direct in vivo gene transfer to urological organs is possible by physical methods like electroporation.…”

Section: Discussionmentioning

confidence: 64%

“…8 It was reported that hole upon a cultured cell perforated with a finely focused laser beam was found to repair itself within a short period of time. Palumbo et al 9 showed that gene transfer in eukaryotic cells was possible by dye-assisted laser optoporation. They also noted that the permeability of the cell membrane is modified at the site of the beam impact.…”

Section: Introductionmentioning

confidence: 99%

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Gene delivery into prostate cancer cells by holmium laser application

Sagi

Knoll

Trojan

et al. 2003

Prostate Cancer Prostatic Dis

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New approaches to treat prostate cancer (PCA) are utilizing gene therapy and aim to correct the disease at the genetic level. Getting a gene efficiently into the target cell is the subject of much interest. We used a holmium laser for transfecting rat PCA cells with the reporter gene pEGFP. By FACS analysis and fluorescence microscopy, we could demonstrate that cellular delivery of plasmid DNA was possible with high efficiencies up to 41.3%. Therefore, transfection of PCA cells by holmium laser might offer a promising new gene transfer strategy to PCA with minimal invasiveness.

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“…In this way, it is possible to introduce impermeable substances such as fl uorophores, dyes and nucleic acids (optical transfection) into the cytosol. This procedure has been applied with CW lasers to transfect cells (Palumbo et al , 1996 ;Schneckenburger et al , 2002 ;Paterson et al , 2005 ;Nikolskaya et al , 2006 ;Torres -Mapa et al, 2010 ). CW sources, which rely on heating to increase membrane permeability, offer high post-transfection viability but low transfection effi ciency.…”

Section: Membranes and Membrane-bound Organellesmentioning

confidence: 99%

At the cutting edge: applications and perspectives of laser nanosurgery in cell biology

Ronchi

Terjung

Pepperkok³

2012

BCHM

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Laser-mediated nanosurgery has become popular in the last decade because of the previously unexplored possibility of ablating biological material inside living cells with sub-micrometer precision. A number of publications have shown the potential applications of this technique, ranging from the dissection of sub-cellular structures to surgical ablations of whole cells or tissues in model systems such as Drosophila melanogaster or Danio rerio . In parallel, the recent development of micropatterning techniques has given cell biologists the possibility to shape cells and reproducibly organize the intracellular space. The integration of these two techniques has only recently started yet their combination has proven to be very interesting. The aim of this review is to present recent applications of laser nanosurgery in cell biology and to discuss the possible developments of this approach, particularly in combination with micropattern-mediated endomembrane organization.

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Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation

Cited by 95 publications

References 20 publications

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Gene delivery into prostate cancer cells by holmium laser application

At the cutting edge: applications and perspectives of laser nanosurgery in cell biology

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