Targeted Metabolomic and Transcript Level Analysis Reveals Quality Characteristic of Chinese Wild Grapes (Vitis davidii Foex)

Abstract

Native to China, spine grapes (Vitis davidii Foex) are an important wild grape species. Here, the quality characteristics of one white and three red spine grape clones were evaluated via targeted metabolomic and transcription level analysis. Xiangzhenzhu (XZZ) had the highest soluble sugar and organic acid content. Malvidin-3-acetyl-glucoside and cyanidin-3-glucoside were the characteristic anthocyanins in spine grapes, and significant differences in anthocyanin composition between different clones were detected. Anthocyanins were not detected in Baiyu (BY) grapes. The transcript levels of VdGST, VdF3′H, VdOMT, VdLDOX, and VdUFGT were significantly related to the anthocyanin biosynthesis and proportions. A total of 27 kinds of glycosidically bound volatiles (including alcohols, monoterpenes, esters, aldehydes, ketones, and phenolic acid) were identified in spine grapes, with Gaoshan #4 (G4) and BY grapes having the highest concentrations. The VdGT expression levels were closely related to glycosidically bound volatile concentrations. These results increase our understanding of the quality of wild spine grapes and further promote the development and use of wild grape resources.

Anthocyanin and Phenolic Acids Contents Influence the Color Stability and Antioxidant Capacity of Wine Treated With Mannoprotein

“…The determination of anthocyanin profiles in wine samples was according to our previous reports (23,24). Briefly, the anthocyanin profiles were analyzed by a high performance liquid chromatography (HPLC) system (Shimadzu Co., Ltd., Kyoto, Japan) fitted with a C 18 column (250 × 4.6 mm; Shimadzu Co., Ltd.).…”

Effects of leaf removal on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in Cabernet Sauvignon (Vitis vinifera L.) grapes

“…4,5 The biosynthesis and accumulation of anthocyanins in red grapes are strongly influenced by genotypes, viticultural practices, and environmental factors such as sunlight exposure, temperature, UV irradiation, and water deficit. 3,[6][7][8][9] It is well known that light plays an important role in forming anthocyanins: shading can decrease the anthocyanin content with lower expressions of regulatory and structural genes, 3,7,10 while moderate exposure of grape berries to solar radiation will change hydroxylation patterns. 9,11 Leaf removal is a management practice adopted with the aim of improving cluster microclimates, to increase the exposure of grapes to sunlight and to reduce the incidence of disease and infection.…”

Effects of methyl jasmonate on the monoterpenes of Muscat Hamburg grapes and wine

“…The VviActin gene was used as a reference control. Gene expression levels were determined with the 2 −ΔΔCt method, with the first control Muscat Hamburg sample serving as the reference 27 …”