Targeted microarray analysis of stationary phase Escherichia coli O157:H7 subjected to disparate nutrient conditions

Allen, Kevin; Lepp, Dion; McKellar, R.C.; Griffiths, Mansel W.

doi:10.1111/j.1365-2672.2010.04843.x

Cited by 13 publications

(12 citation statements)

References 35 publications

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“…This is another argument that confirms the validity of our microarray 363 17 results, as already described in similar works (Allen et al 2010;Klitgaard et al 2010; 364 Vasileva et al 2012). 365…”

Section: Discussion 340supporting

confidence: 80%

“…The range of log 2 fold changes was apparently low, ranging between 357 1.78 and -2.13, but the numbers obtained were in general quite similar to those observed for 358 transcriptomic analyses of iron starvation in other microorganisms (Allen et al 2010; Basler 359 et al 2006;Brickman et al 2011;Klitgaard et al 2010;Madsen et al 2006). However, very 360 interestingly the changes observed in our work were in most cases for genes grouped in 361 operons, and genes belonging to the same operon underwent, as expected, almost identical 362…”

Section: Discussion 340mentioning

confidence: 53%

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Peer Review #1 of "Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 (v0.2)"

2018

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17We applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study 18 the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae 19 to elucidate global changes in the bacterium in a condition similar to what can be found in the 20 host during an infectious episode. We treated the reference strain TIGR4 with the iron 21 chelator deferoxamine mesylate. DNA microarrays revealed changes in the expression of 22 operons involved in multiple biological processes, with a prevalence of genes coding for ion 23 binding proteins. We also studied the changes in protein abundance by 2-DE followed by 24 MALDI-TOF/TOF analysis of total cell extracts and secretome fractions. The main proteomic 25 changes were found in proteins related to the primary and amino sugars metabolism, 26 especially in enzymes with divalent cations as cofactors. Finally, the metabolomic analysis of 27 intracellular metabolites showed altered levels of amino sugars involved in the cell wall 28peptidoglycan metabolism. This work shows the utility of multi-perspective studies that can 29 provide complementary results for the comprehension of how a given condition can influence 30 on global physiological changes in microorganisms. 31 32 3 Introduction 33Bacteria need a plethora of factors for optimal growth, being iron an essential micronutrient. 34Within the human body, the free concentration of this element is approximately 10 -18 M (Yang 35 et al. 2014), which is too low to hold a growth capable of supporting bacterial infections. The 36 low concentrations of free iron within the host are due to the scavenging of this element by 37 different high-affinity proteins (e.g. transferrin, lactoferrin, haemoglobin, etc.) (Froehlich et 38 al. 2009). Therefore, in order to survive in the host, pathogens need to develop especial 39 strategies to uptake the minimum amount that they require of such a nutrient (Nanduri et al. 40 2008), as the direct extraction of this metal cation from host iron-containing proteins or by 41 capturing ferric-binding siderophores from host environments via ABC transporters (Ge & 42 Sun 2014). Moreover, the capacity of bacterial pathogens for iron acquisition represents itself 43 an important virulence determinant (Kanaujia et al. 2015). In addition, pathogens can also 44 modify their energetic metabolism to adapt it to the environmental situation within the host. 45Streptococcus pneumoniae, also known as the pneumococcus, is a Gram-positive 46 microorganism that lives as a commensal in the human respiratory tract and that, under 47 appropriate circumstances, becomes pathogenic, being able to cause high morbidity and 48 mortality (Blasi et al. 2012; Olaya-Abril et al. 2014b). This bacterium is a major cause of 49 mucosal diseases such as otitis media and sinusitis, and is a prevalent pathogen in different 50 invasive diseases including pneumonia, bacteremia, meningitis, and sepsis (O'Brien et al. 512009). Pneumococcal pneumonia, which is the major clinical manife...

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Section: Discussion 340supporting

confidence: 80%

Section: Discussion 340mentioning

confidence: 53%

Peer Review #1 of "Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 (v0.2)"

2018

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“…Therefore, decreased heat tolerance may occur due to the reduced melting point of unsaturated fatty acids within the cell membrane. Other scientist have also indicated that the stress response of bacteria to sublethal environmental stresses such as changes in temperature, starvation, or high osmolarity may provide cross-protection to a variety of postphysical and postchemical stresses including heat and acid (Allen, Lepp, McKellar, & Griffiths, 2010;Arnold & Kaspar, 1995;Erdoğrul, Erbilir, & Toroğlu, 2006;House et al, 2009;Jeong, Baumler, & Kaspar, 2006;Lange & Hengge-Aronis, 1994;Leenanon & Drake, 2001;Nair & Finkel, 2004).…”

Section: Effect Of Ph and Thermal Stress On The Thermal Tolerance Omentioning

confidence: 99%

Influence of prior pH and thermal stresses on thermal tolerance of foodborne pathogens

Kim

Bushlaibi

Alrefaei

et al. 2019

Food Science & Nutrition

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Improper food processing is one of the major causes of foodborne illness. Accurate prediction of the thermal destruction rate of foodborne pathogens is therefore vital to ensure proper processing and food safety. When bacteria are subjected to pH and thermal stresses during growth, sublethal stresses can occur that may lead to differences in their subsequent tolerance to thermal treatment. As a preliminary study to test this concept, the current study evaluated the effect of prior pH and thermal stresses on thermal tolerance of Salmonella and Staphylococcus using a tryptic soy broth supplemented with yeast extract. Bacteria incubated at three pH values (6.0, 7.4, and 9.0) and four temperatures (15, 25, 35, and 45°C) for 24 hr were subjected to thermal treatments at 55, 60, and 65°C. At the end of each treatment time, bacterial suspensions were surface‐plated on standard method agar for quantification of bacterial survival and further calculation of the thermal death decimal reduction time ( D ‐value) and thermal destruction temperature ( z ‐value). The effect of pH stress alone during the incubation on the thermal tolerance of both bacteria was generally insignificant. An increasing pattern of D ‐value was observed with the increment of thermal stress (incubation temperature). The bacteria incubated at 35°C required the highest z ‐value to reduce the 90% in D ‐values . Staphylococcus mostly displayed higher tolerance to thermal treatment than Salmonella . Although further research is needed to validate the current findings on food matrices, findings in this study clearly affirm that adaptation of bacteria to certain stresses may reduce the effectiveness of preservation procedures applied during later stage of food processing and storage.

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“…Duffitt et al (8) showed that in water and sterile soil, E. coli 0157 developed a more virulent phenotype, including a higher level of expression of virulence genes. E. coli 0157:H7 also undergoes significant but low induction of genes involved in the stress response and virulence during starvation (1).…”

Section: Discussionmentioning

confidence: 99%

Glucose Decreases Virulence Gene Expression of Escherichia coli O157:H7

Delcenserie

LaPointe

Charaslertrangsi

et al. 2012

Journal of Food Protection

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Escherichia coli O157:H7 is responsible for a human toxico-infection that can lead to severe complications such as hemolytic uremic syndrome. Inside the intestine, E. coli O157:H7 forms typical attaching-effacing lesions and produces Shiga toxins. The genes that are responsible for these lesions are located in a pathogenicity island called the locus of enterocyte effacement (LEE). LEE gene expression is influenced by quorum sensing through the luxS system. In this study, the effect of glucose on the expression of several genes from LEE, on the expression of Shiga toxin genes, and on the expression of luxS was assessed with real-time, reverse transcription PCR. All concentrations of glucose (from 0.1 to 1%) were able to down-regulate genes from the LEE operon. A slight down-regulation of genes implicated in Shiga toxin expression was also observed but was significant for low doses of glucose (0.1 to 0.5%) only. A slight but significant increase in luxS expression was observed with 1% glucose. This confirms that in addition to quorum sensing, the presence or absence of nutrients such as glucose has an impact on the down- or upregulation of LEE-encoded virulence genes by the bacterium. The influence of glucose on the virulence of E. coli O157:H7 has received little attention, and these results suggest that glucose can have an important effect on the virulence of E. coli O157:H7.

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Targeted microarray analysis of stationary phase Escherichia coli O157:H7 subjected to disparate nutrient conditions

Cited by 13 publications

References 35 publications

Peer Review #1 of "Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 (v0.2)"

Peer Review #1 of "Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 (v0.2)"

Influence of prior pH and thermal stresses on thermal tolerance of foodborne pathogens

Glucose Decreases Virulence Gene Expression of Escherichia coli O157:H7

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