Targeted photodynamic therapy

Solban, Nicolas; Rizvi, Imran; Hasan, Tayyaba

doi:10.1002/lsm.20345

Cited by 143 publications

(103 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that PDT damages the microvasculature and induces a vascular shut-down effect (3,12,30). We examined whether induction of vascular endothelial growth factor (VEGF) expression in the LLC/IL-2 tumors may be involved in the enhanced angiogenesis and tumor recurrence following NPe6-PDT.…”

Section: Overexpression Of Il-2 Induced and Promoted Gadd-45αmentioning

confidence: 99%

High expression of GADD-45α and VEGF induced tumor recurrence via upregulation of IL-2 after photodynamic therapy using NPe6

Ohtani

Usuda²,

Ichinose³

et al. 2008

Int J Oncol

View full text Add to dashboard Cite

Abstract. NPe6 is a novel second-generation photosensitizer used for photodynamic therapy (PDT). PDT using NPe6 and diode laser (664 nm) induces cell death, inflammatory reactions, immunological responses and damage to the microvasculature. In this study, we evaluated the influence of the immunological responses and of enhanced angiogenesis on the anti-tumor effect of NPe6-PDT using cytokine-overexpressing Lewis lung carcinoma (LLC), LLC-IL-2 cells both in vitro and in vivo. We showed by DNA microarray analysis in vitro that IL-2 and GADD-45α (growth arrest and DNA damage 45 alpha) mRNA expressions were induced by 3 h after NPe6-PDT applied at a dose killing 90% of the cells (LD 90 ). IL-2-overexpressing cells (LLC/IL-2 cells) were resistant to the loss of clonogenicity as compared to the parental LLC cells in vitro. Furthermore, in female C57BL/6 mice, NPe6-PDT produced a cure rate of 66.7% in LLC tumors, whereas the cure rate was only 16.6% in LLC/IL-2 tumors, and overexpression of IL-2 caused failure of NPe6-PDT, with tumor recurrence, in vivo. These results suggest that IL-2 expression may play an unfavorable role in attenuation of the antitumor effect of NPe6-PDT. It has been reported that the expression of vascular endothelial growth factor (VEGF), in particular, may cause tumor recurrence after PDT and exert unfavorable effect in relation to attenuate the anti-tumor activity of PDT. Results of immunohistochemical analysis of LLC/IL-2 tumors have revealed that the expressions of GADD-45α and VEGF are induced in these tumors after PDT, and in particular, 12 h after PDT, the expression levels were much higher as compared with those in the LLC tumors. The results of our studies using in vitro and in vivo models suggest that the cell death caused by PDT was inhibited by induction of GADD-45α expression and that tumor recurrence was promoted by the enhancement of VEGF expression mediated by IL-2 upregulation. Therefore, it is speculated that the use of an IL-2 inhibitor may improve the efficacy of NPe6-PDT.

show abstract

Section: Overexpression Of Il-2 Induced and Promoted Gadd-45αmentioning

confidence: 99%

High expression of GADD-45α and VEGF induced tumor recurrence via upregulation of IL-2 after photodynamic therapy using NPe6

Ohtani

Usuda²,

Ichinose³

et al. 2008

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…PDT has the advantage of dual selectivity in that the photosensitizer can be targeted to a destination cell or tissue, and in addition the illumination can be spatially directed to the lesion (7,48). PDT has its origins over a hundred years ago in the discovery of light-mediated killing of microorganisms (35) but since then has been principally developed as a treatment for cancer (8) and age-related macular degeneration (58).…”

mentioning

confidence: 99%

Stable Synthetic Cationic Bacteriochlorins as Selective Antimicrobial Photosensitizers

Huang

Mróz

et al. 2010

Antimicrob Agents Chemother

120

112

View full text Add to dashboard Cite

Photodynamic inactivation is a rapidly developing antimicrobial treatment that employs a nontoxic photoactivatable dye or photosensitizer in combination with harmless visible light to generate reactive oxygen species that are toxic to cells. Tetrapyrroles (e.g., porphyrins, chlorins, bacteriochlorins) are a class of photosensitizers that exhibit promising characteristics to serve as broad-spectrum antimicrobials. In order to bind to and efficiently penetrate into all classes of microbial cells, tetrapyrroles should have structures that contain (i) one or more cationic charge(s) or (ii) a basic group. In this report, we investigate the use of new stable synthetic bacteriochlorins that have a strong absorption band in the range 720 to 740 nm, which is in the near-infrared spectral region. Four bacteriochlorins with 2, 4, or 6 quaternized ammonium groups or 2 basic amine groups were compared for light-mediated killing against a Gram-positive bacterium (Staphylococcus aureus), a Gramnegative bacterium (Escherichia coli), and a dimorphic fungal yeast (Candida albicans). Selectivity was assessed by determining phototoxicity against human HeLa cancer cells under the same conditions. All four compounds were highly active (6 logs of killing at 1 M or less) against S. aureus and showed selectivity for bacteria over human cells. Increasing the cationic charge increased activity against E. coli. Only the compound with basic groups was highly active against C. albicans. Supporting photochemical and theoretical characterization studies indicate that (i) the four bacteriochlorins have comparable photophysical features in homogeneous solution and (ii) the anticipated redox characteristics do not correlate with cell-killing ability. These results support the interpretation that the disparate biological activities observed stem from cellular binding and localization effects rather than intrinsic electronic properties. These findings further establish cationic bacteriochlorins as extremely active and selective near-infrared activated antimicrobial photosensitizers, and the results provide fundamental information on structure-activity relationships for antimicrobial photosensitizers.

show abstract

“…В экспериментах H. Solhan et al тестировали эффективность моноклональных анти-EGFR антител, свя-занных с хлорином e6 при различных условиях [48].…”

Section: возможности проведения интраоперационной фотодинамической теunclassified

Intraoperative Photodynamic Therapy for Metastatic Peritoneal Tumors

Suleymanov¹,

Хомяков²,

Серова³

et al. 2016

Issled. prakt. med. (Print)

View full text Add to dashboard Cite

РЕЗЮМЕНастоящий обзор посвящен проблеме циторедуктивного лечения при злокачественной опухолевой патологии органов брюшной полости. Актуальность проблемы обусловлена как ростом заболеваемости раком данной локализации в России и в большинстве развитых стран мира, так и высокой частотой выявления заболева-ния на поздних стадиях. Описаны методы специфического лечения канцероматоза брюшины, основанные на возможности воздействия на очаги микродиссеминации после выполненной хирургической максималь-ной циторедукции. К данным методикам дополнительного интраоперационного специфического противоопу-холевого воздействия относятся интраоперационная лучевая терапия, гипертермическая внутрибрюшная химиотерапия, интраоперационная фотодинамическая терапия, каждая из которых отличается уровнем сложности исполнения, механизмами воздействия на опухоль и здоровые ткани больного, эффективностью. В обзоре наиболее подробно описаны преимущества, перспективы и возможности применения интраопера-ционной фотодинамической терапии (ИОФДТ) при перитонеальном канцероматозе, приведены результаты ряда отечественных и зарубежных клинических исследований, продемонстрировано успешное применение ИОФДТ в клинической онкологии, позволяющее существенно снизить риск развития вторичного опухолевого поражения брюшины. Фотодинамическая терапия -метод, обладающий высокой эффективностью и практи-чески не имеющий побочных эффектов и осложнений, основанный на способности фотосенсибилизаторов селективно накапливаться и удерживаться в высокопролиферативных тканях. Преимуществами данного вида лечения больных перитонеальным канцероматозом являются селективность воздействия на скрытые очаги микродиссеминации, а также визуально определяющуюся опухолевую ткань, высокая эффективность исполь-зования у больных злокачественными новообразованиями органов брюшной полости и малого таза в соче-тании с циторедуктивным лечением, минимальное влияние на здоровые органы и ткани пациента, хорошая переносимость процедуры.КЛЮЧЕВЫЕ СЛОВА: интраоперационная фотодинамическая терапия, канцероматоз брюшины, колоректальный рак, рак желудка, рак яичников

show abstract

Targeted photodynamic therapy

Cited by 143 publications

References 61 publications

High expression of GADD-45α and VEGF induced tumor recurrence via upregulation of IL-2 after photodynamic therapy using NPe6

High expression of GADD-45α and VEGF induced tumor recurrence via upregulation of IL-2 after photodynamic therapy using NPe6

Stable Synthetic Cationic Bacteriochlorins as Selective Antimicrobial Photosensitizers

Intraoperative Photodynamic Therapy for Metastatic Peritoneal Tumors

Contact Info

Product

Resources

About