Targeted Profiling of Heat Shock Proteome in Radioresistant Breast Cancer Cells

Miao, Weili; Fan, Ming; Huang, Ming Shyan; Li, Jian Jian; Wang, Yinsheng

doi:10.1021/acs.chemrestox.8b00330

Cited by 14 publications

(8 citation statements)

References 44 publications

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“…Thus, radiation therapy might be better delivered to ER + and cyclin D1-positive BCs along with estradiol itself, rather than in combination with anti-estrogenic agents [ 109 ]. Furthermore, HSPB8 expression is markedly elevated in radioresistant BC lines compared to parental MCF-7 and MDA-MB-231 cells, revealing the reprogramming of the heat shock proteome during the development of radioresistance in BC [ 111 ].…”

Section: Role Of Hspb8 In Cancer Development and Progressionmentioning

confidence: 99%

The Role of HSPB8, a Component of the Chaperone-Assisted Selective Autophagy Machinery, in Cancer

Cristofani¹,

Piccolella²,

Crippa³

et al. 2021

Cells

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The cellular response to cancer-induced stress is one of the major aspects regulating cancer development and progression. The Heat Shock Protein B8 (HSPB8) is a small chaperone involved in chaperone-assisted selective autophagy (CASA). CASA promotes the selective degradation of proteins to counteract cell stress such as tumor-induced stress. HSPB8 is also involved in (i) the cell division machinery regulating chromosome segregation and cell cycle arrest in the G0/G1 phase and (ii) inflammation regulating dendritic cell maturation and cytokine production. HSPB8 expression and role are tumor-specific, showing a dual and opposite role. Interestingly, HSPB8 may be involved in the acquisition of chemoresistance to drugs. Despite the fact the mechanisms of HSPB8-mediated CASA activation in tumors need further studies, HSPB8 could represent an important factor in cancer induction and progression and it may be a potential target for anticancer treatment in specific types of cancer. In this review, we will discuss the molecular mechanism underlying HSPB8 roles in normal and cancer conditions. The basic mechanisms involved in anti- and pro-tumoral activities of HSPB8 are deeply discussed together with the pathways that modulate HSPB8 expression, in order to outline molecules with a beneficial effect for cancer cell growth, migration, and death.

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Section: Role Of Hspb8 In Cancer Development and Progressionmentioning

confidence: 99%

The Role of HSPB8, a Component of the Chaperone-Assisted Selective Autophagy Machinery, in Cancer

Cristofani¹,

Piccolella²,

Crippa³

et al. 2021

Cells

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“…Similar activities, responses and subcellular localizations are also attributed to another member of the HSPA subfamily such as HSPA14 (HSP70L1). There are data that suggest the acquisition of radioresistance by human breast cancer cells is accompanied by the enhancement of HSPA14 expression [ 16 ]; however, a causal link between the intratumoral HSPA14 level and tumor radiation response has not been yet established. Together with HSP90, HSC70 binds to and inhibits HSF1, thus preventing the HSF1 activation under normal conditions.…”

Section: Members Of Hsp70 Subfamily: General Characteristics Localization Functioningmentioning

confidence: 99%

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy

Kabakov

Gabai²

2021

Cells

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The high frequency of breast cancer worldwide and the high mortality among women with this malignancy are a serious challenge for modern medicine. A deeper understanding of the mechanisms of carcinogenesis and emergence of metastatic, therapy-resistant breast cancers would help development of novel approaches to better treatment of this disease. The review is dedicated to the role of members of the heat shock protein 70 subfamily (HSP70s or HSPA), mainly inducible HSP70, glucose-regulated protein 78 (GRP78 or HSPA5) and GRP75 (HSPA9 or mortalin), in the development and pathogenesis of breast cancer. Various HSP70-mediated cellular mechanisms and pathways which contribute to the oncogenic transformation of mammary gland epithelium are reviewed, as well as their role in the development of human breast carcinomas with invasive, metastatic traits along with the resistance to host immunity and conventional therapeutics. Additionally, intracellular and cell surface HSP70s are considered as potential targets for therapy or sensitization of breast cancer. We also discuss a clinical implication of Hsp70s and approaches to targeting breast cancer with gene vectors or nanoparticles downregulating HSP70s, natural or synthetic (small molecule) inhibitors of HSP70s, HSP70-binding antibodies, HSP70-derived peptides, and HSP70-based vaccines.

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“…The radioresistant clone (clone 6 or C6) of MCF-7 cells was produced by treating the parental cells with a total of 60 Gy of γ rays and further exposed to an additional term of 30 fractions of γ rays. 17,18 Similarly, MDA-MB-231 cells were exposed to a combined dose of 30 Gy of ionizing radiation to yield the radioresistant C5 clone. 18,19 The details for cell culture conditions are provided in the Supporting Information.…”

Section: Cell Culturementioning

confidence: 99%

“…17,18 Similarly, MDA-MB-231 cells were exposed to a combined dose of 30 Gy of ionizing radiation to yield the radioresistant C5 clone. 18,19 The details for cell culture conditions are provided in the Supporting Information. Approximately 5 × 10 6 cells were harvested by trypsinization, washed twice with cold phosphatebuffered saline (PBS), and lysed.…”

Section: Cell Culturementioning

confidence: 99%

Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells

2021

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Radiotherapy constitutes a major therapeutic modality for early management of breast cancer. Despite the high efficacy in treating breast cancer (BC), radiation resistance and tumor recurrence are major hurdles in breast cancer radiotherapy. Herein, stable isotope labeling by amino acids in cell culture (SILAC) was employed, along with the parallel-reaction monitoring (PRM)-based targeted quantitative proteomic method, to examine the differences in kinase protein expression in MCF-7 and MDA-MB-231 breast cancer cells and their corresponding radioresistant C6 and C5 clones. We quantified the relative protein expression levels of 300 and 281 kinases in C5/MDA-MB-231 and C6/MCF-7 pairs of breast cancer cells, respectively. We also showed that TAF9, which was one of the differentially expressed kinases, enhances radiation resistance in breast cancer cells. Moreover, a correlation analysis of gene expression suggested TAF9’s role in upregulating the expression of genes involved with radioresistance. Overall, our study uncovered a large number of differentially expressed kinases accompanied with the acquisition of radioresistance and revealed a role of TAF9 in promoting radioresistance in breast cancer.

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Targeted Profiling of Heat Shock Proteome in Radioresistant Breast Cancer Cells

Cited by 14 publications

References 44 publications

The Role of HSPB8, a Component of the Chaperone-Assisted Selective Autophagy Machinery, in Cancer

The Role of HSPB8, a Component of the Chaperone-Assisted Selective Autophagy Machinery, in Cancer

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy

Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells

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