Targeting collagen damage for sustained in situ antimicrobial activities

Mo, Xiaoyun; Zhao, Suwen; Zhao, Jie; Huang, Yongjie; Li, Tao; Zhu, Yong‐Guan; Li, Gang; Li, Yang; Shan, Hong

doi:10.1016/j.jconrel.2023.06.013

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…We next performed histological analysis of the wound tissues using hematoxylin and eosin (H&E staining) and Masson trichrome staining methods to observe inflammatory cell infiltration (H&E staining), [ 67 ] and collagen deposition (Masson staining) in the oral mucosal wounds after 5 days of treatment (Figure 5H ). [ 68 ] Collagen fibers serve as a cellular scaffold in the granulation tissue and promote the formation of new blood vessels and epithelial coverage. The PBS group exhibited fewer collagen fibers and a loose mesh arrangement.…”

Section: Resultsmentioning

confidence: 99%

A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

Wu,

Xu,

et al. 2023

Advanced Science

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Candida albicans (C. albicans), a ubiquitous polymorphic fungus in humans, causes different types of candidiasis, including oral candidiasis (OC) and vulvovaginal candidiasis (VVC), which are physically and mentally concerning and financially costly. Thus, developing alternative antifungals that prevent drug resistance and induce immunity to eliminate Candida biofilms is crucial. Herein, a novel membrane‐targeted aggregation‐induced emission (AIE) photosensitizer (PS), TBTCP‐QY, is developed for highly efficient photodynamic therapy (PDT) of candidiasis. TBTCP‐QY has a high molar absorption coefficient and an excellent ability to generate 1O2 and •OH, entering the interior of biofilms due to its high permeability. Furthermore, TBTCP‐QY can efficiently inhibit biofilm formation by suppressing the expression of genes related to the adhesion (ALS3, EAP1, and HWP1), invasion (SAP1 and SAP2), and drug resistance (MDR1) of C. albicans, which is also advantageous for eliminating potential fungal resistance to treat clinical infectious diseases. TBTCP‐QY‐mediated PDT efficiently targets OC and VVC in vivo in a mouse model, induces immune response, relieves inflammation, and accelerates the healing of mucosal defects to combat infections caused by clinically isolated fluconazole‐resistant strains. Moreover, TBTCP‐QY demonstrates excellent biocompatibility, suggesting its potential applications in the clinical treatment of OC and VVC.

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Section: Resultsmentioning

confidence: 99%

A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

Wu,

Xu,

et al. 2023

Advanced Science

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Action Programmed Nanoantibiotics with pH‐Induced Collapse and Negative‐Charged‐Surface‐Induced Deformation against Antibiotic‐Resistant Bacterial Peritonitis

Zhang,

Liu,

Zhang

et al. 2024

Adv Healthcare Materials

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The incorporation of well‐designed antibiotic nanocarriers, along with an antibiotic adjuvant effect, in combination with various antibiotics, offers an opportunity to combat drug‐resistant strains. However, precise control over morphology and encapsulated payload release can significantly impact their antibacterial efficacy and synergistic effects when used alongside antibiotics. Here, this study focuses on developing lipopeptide‐based nanoantibiotics, which demonstrate an antibiotic adjuvant effect by inducing pH‐induced collapse and negative‐charged‐surface‐induced deformation. This enhances the disruption of the bacterial outer membrane and facilitates drug penetration, effectively boosting the antimicrobial activity against drug‐resistant strains. The modulation regulations of the lipopeptide nanocarriers with modular design are governed by the authors. The nanoantibiotics, made from lipopeptide and ciprofloxacin (Cip), have a drug loading efficiency of over 80%. The combination with Cip results in a significantly low fractional inhibitory concentration index of 0.375 and a remarkable reduction in the minimum inhibitory concentration of Cip against multidrug‐resistant (MDR) Escherichia coli (clinical isolated strains) by up to 32‐fold. The survival rate of MDR E. coli peritonitis treated with nanoantibiotics is significantly higher, reaching over 87%, compared to only 25% for Cip and no survival for the control group. Meanwhile, the nanoantibiotic shows no obvious toxicity to major organs.

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The Guardian of Vision: Intelligent Bacteriophage‐Based Eyedrops for Clinical Multidrug‐Resistant Ocular Surface Infections

Chen,

Wu,

Chen

et al. 2024

Advanced Materials

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Clinical multidrug‐resistant Pseudomonas aeruginosa (MDR‐PA) is the leading cause of refractory bacterial keratitis (BK). However, the reported BK treatment methods lack biosecurity and bioavailability, which usually causes irreversible visual impairment and even blindness. Herein, for BK caused by clinically isolated MDR‐PA infection, armed phages are modularized with the type I photosensitizer (PS) ACR‐DMT, and an intelligent phage eyedrop is developed for combined phagotherapy and photodynamic therapy (PDT). These eyedrops maximize the advantages of bacteriophages and ACR‐DMT, enabling more robust and specific targeting killing of MDR‐PA under low oxygen‐dependence, penetrating and disrupting biofilms, and efficiently preventing biofilm reformation. Altering the biofilm and immune microenvironments alleviates inflammation noninvasively, promotes corneal healing without scar formation, protects ocular tissues, restores visual function, and prevents long‐term discomfort and pain. This strategy exhibits strong scalability, enables at‐home treatment of ocular surface infections with great patient compliance and a favorable prognosis, and has significant potential for clinical application.

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Targeting collagen damage for sustained in situ antimicrobial activities

Cited by 4 publications

References 54 publications

A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

Action Programmed Nanoantibiotics with pH‐Induced Collapse and Negative‐Charged‐Surface‐Induced Deformation against Antibiotic‐Resistant Bacterial Peritonitis

The Guardian of Vision: Intelligent Bacteriophage‐Based Eyedrops for Clinical Multidrug‐Resistant Ocular Surface Infections

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