Targeting Heat Shock Protein 70 as an antiviral strategy against grass carp reovirus infection

Shan, Li-Peng; Chen, Xiaohui; Ling, Fei; Zhu, Bin; Wang, Gao‐Xue

doi:10.1016/j.virusres.2018.01.005

Cited by 24 publications

(18 citation statements)

References 31 publications

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“…Besides heat stress (Baena et al, 2018; Sales et al, 2018), heat shock proteins (HSP) are being evaluated in apoptosis, oxidative stress, inflammatory diseases, cancer (Ikwegbue et al, 2017), virus infection (Shan et al, 2018), bacterial infection (Kim et al, 2018), as immunomodulators (Edkins et al, 2017; Zininga et al, 2018), therapeutics (Skórzynska-Dziduszko et al, 2018), and diagnostics (Lechner et al, 2018; Tang T. et al, 2018). HSPs have diagnostic role in ischemic injury of cardiomyocytes (Santos et al, 2018), inflammatory process of multiple sclerosis (Lechner et al, 2018), early diagnosis of lung cancer (Tang T. et al, 2018), proteome stress (Liu and Zhang, 2018), bipolar disorder (Cheng et al, 2018), and anti-apoptotic agent (Jang et al, 2018).…”

Section: Heat Shock Proteins (Hsps)mentioning

confidence: 99%

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Dhama

Latheef

Dadar

et al. 2019

Front. Mol. Biosci.

221

172

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Various internal and external factors negatively affect the homeostatic equilibrium of organisms at the molecular to the whole-body level, inducing the so-called state of stress. Stress affects an organism's welfare status and induces energy-consuming mechanisms to combat the subsequent ill effects; thus, the individual may be immunocompromised, making them vulnerable to pathogens. The information presented here has been extensively reviewed, compiled, and analyzed from authenticated published resources available on Medline, PubMed, PubMed Central, Science Direct, and other scientific databases. Stress levels can be monitored by the quantitative and qualitative measurement of biomarkers. Potential markers of stress include thermal stress markers, such as heat shock proteins (HSPs), innate immune markers, such as Acute Phase Proteins (APPs), oxidative stress markers, and chemical secretions in the saliva and urine. In addition, stress biomarkers also play critical roles in the prognosis of stress-related diseases and disorders, and therapy guidance. Moreover, different components have been identified as potent mediators of cardiovascular, central nervous system, hepatic, and nephrological disorders, which can also be employed to evaluate these conditions precisely, but with stringent validation and specificity. Considerable scientific advances have been made in the detection, quantitation, and application of these biomarkers. The present review describes the current progress of identifying biomarkers, their prognostic, and therapeutic values.

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Section: Heat Shock Proteins (Hsps)mentioning

confidence: 99%

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Dhama

Latheef

Dadar

et al. 2019

Front. Mol. Biosci.

221

172

View full text Add to dashboard Cite

show abstract

“…Hsp70 is involved in immune responses and participates in the life of a virus through folding, transporting, positioning, assembling, or degrading activities (Risler et al, 2012). Increasing evidence suggests that Hsp70 plays key roles in viral replication and assembly in several RNA viruses (Nagy et al, 2011; Lahaye et al, 2012; Katoh et al, 2017), such as those of dengue (Taguwa et al, 2015), avian infectious bronchitis (Shan et al, 2018), and influenza A (Manzoor et al, 2014). Alzhanova et al (2014) observed that Hsp70 could bind to the structural proteins of beet yellows virus to form a complex and participate in viral assembly.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Novel Host Protein Interacting With the Structural Protein VP1 of Chinese Sacbrood Virus by Yeast Two-Hybrid Screening

et al. 2019

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Chinese sacbrood virus (CSBV) is the major cause and lead to the collapse of Apis cerana colonies. VP1, the structural protein of CSBV, shows the highest variation in the amino acid sequences among proteins from different CSBV strains as well as exhibits excellent immunogenicity. However, its function with host protein still remains unclear. To clarify its function with host protein, we screened out host cellular proteins that interact with VP1 using the membrane protein yeast two-hybrid system. In addition, we verified interactions between heat shock protein 70 cognate 5 (Hsp70-c5) and VP1 using glutathione S-transferase (GST) pull-down and co-immunoprecipitation assays. VP1 and Hsp70-c5 were colocalized in the cytoplasm and nucleus. Using western blot and real-time polymerase chain reaction (PCR), Hsp70-c5 expression in CSBV-infected larvae was upregulated compared with that in healthy larvae. We observed that when we silenced Hsp70-c5, VP1 expression was significantly downregulated. These results demonstrate that Hsp70-c5 is involved in at least one stage(s) of the viral life cycle.

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“…sense (-), ssRNA families, including Ebola virus from the Filoviridae (García-Dorival et al, 2016), mumps virus (MuV) from the Paramyxoviridae (Katoh et al, 2017), human respiratory syncytial virus (HRSV) from the Pneumoviridae (Munday et al, 2015), and enterovirus A71 (EV-A71) from the Picornaviridae (Dong et al, 2018). The only fish virus studied was grass carp reovirus, a member of the double-stranded (ds) RNA Reoviridae family (Shan et al, 2018).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

VER-155008 induced Hsp70 proteins expression in fish cell cultures while impeding replication of two RNA viruses

et al. 2019

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The heat-shock protein 70 (Hsp70) inhibitor, VER-155008 (VER), was explored as a potential antiviral agent for two RNA viruses important to fish aquaculture, viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV). Studies were done at a temperature of 14 °C, and with cell lines commonly used to propagate these viruses. These were respectively EPC from fathead minnow for VHSV and CHSE-214 from Chinook salmon embryo for IPNV. Additionally, both viruses were studied with the Atlantic salmon heart endothelial cell line ASHe. For both VHSV and IPNV, 25 µM VER impeded replication. This was evidenced by delays in the development of cytopathic effect (CPE) and the expression of viral proteins, N for VHSV and VP2 for IPNV, and by less production of viral RNA and of viral titre. As VER inhibits the activity of Hsp70 family members, these results suggest that VHSV and IPNV utilize one or more Hsp70s in their life cycles. Yet neither virus induced Hsp70. Surprisingly VER alone induced Hsp70, but whether this induction modulated VER's antiviral effects is unknown. Exploring this apparent paradox in the future should improve the usefulness of VER as an antiviral agent.

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Targeting Heat Shock Protein 70 as an antiviral strategy against grass carp reovirus infection

Cited by 24 publications

References 31 publications

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Identification of the Novel Host Protein Interacting With the Structural Protein VP1 of Chinese Sacbrood Virus by Yeast Two-Hybrid Screening

VER-155008 induced Hsp70 proteins expression in fish cell cultures while impeding replication of two RNA viruses

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